DETERMINATION OF OXYGEN EQUILIBRIA WITH A VERSATILE NEW TONOMETER.

Abstract A spectrophotometric method is deseribed for determining oxygen equilibria of hemoglobin in concentrations ranging from 0.005 to 10%. A new tonometer and rotating assembly were devised to carry out these measurements. Equations are given for calculating the results in the various concentration ranges and for detecting impurities of methemoglobin. It is also shown how methemoglobin formation can be kept to a minimum in work with very dilute hemoglobin solutions.