The effect of whey protein hydrolyzates on the lactic acid fermentation

SummaryThe batch fermentation of whey permeate to lactic acid was improved markedly by the addition of enzymehydrolyzed whey protein. Acid concentrations greater than 90 g/l were achieved at a productivity of 4.3 g/l per h and a 98% substrate use. Cell mass concentration reached 6 g/l. The acid productivity achieved is somewhat higher than that typical for fermentation of whole whey. The process economics, based on in-house hydrolyzate preparation, look promising. Presented in this paper are the experimental results showing the effects of hydrolyzate concentration on acid and cell mass production.

[1]  W. Sandine,et al.  Identification of a Growth Stimulant for Lactobacillus sanfrancisco , 1981, Applied and environmental microbiology.

[2]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[3]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[4]  E. Snell,et al.  Peptides and bacterial growth. VIII. The nature of strepogenin. , 1960, The Journal of biological chemistry.

[5]  H. Kihara.  PEPTIDES AND BACTERIAL GROWTH , 2001 .

[6]  J. M. Prescott,et al.  Peptides and bacterial growth. IV. Histidine peptides as growth factors for Lactobacillus delbrueckii 9649. , 1953, The Journal of biological chemistry.

[7]  P. Gerhardt,et al.  Continuous Process for Ammonium-Lactate Fermentation of Deproteinized Whey , 1979 .

[8]  R. B. Merrifield,et al.  Specificity of Peptides , 1958, Science.

[9]  M. Cheryan,et al.  Lactic acid from acid whey permeate in a membrane recycle bioreactor , 1986 .

[10]  J. M. Prescott,et al.  Peptides and bacterial growth. V. Serine peptides and growth of Lactobacillus delbrueckii 9649. , 1953, The Journal of biological chemistry.

[11]  J. Spies.  [76] Colorimetric procedures for amino acids , 1957 .

[12]  M. Desmazeaud.  L'état des connaissances en matière de nutrition des bactéries lactiques , 1983 .

[13]  E. Snell,et al.  Peptides and bacterial growth. VI. The nutritional requirements of Lactobacillus delbrueckii. , 1954, Journal of bacteriology.

[14]  H. Blanch,et al.  Continuous production of lactic acid from glucose and lactose in a cell-recycle reactor , 1985 .

[15]  Merrifield Rb.  Competitive inhibition of a strepogenin-active peptide by related peptides. , 1958 .