Supercritical fluid fractionation of fish oil esters using incremental pressure programming and a temperature gradient

Incremental pressure programming was demonstrated to be an effective technique for increasing the yield of 90% pure allcis-5,8,11,14,17-ethyl eicosapentaenoate (EPA) in the fractionation of urea-crystallized fish oil ethyl esters using supercritical fluid CO2. The fractionations, which also produced high purity allcis-4,7,10,13,16,19-ethyl docosahexaenoate (DHA), were performed using a column temperature gradient. In initial experiments, the maximum temperature of the superimposed gradient was 80°C, and processing pressures ranged from 1900–2200 psi. By reduction of processing pressures, comparable yields of EPA were obtained from fractionations in which the maximum temperatures in the gradient were 70°C and 60°C.

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