Revista Mexicana de I ngeniería Q uímica CONTENIDO Interest of cellular di ff erentiation in the production of vincristine and vinblastine in suspension cultures of Catharanthus roseus (L.) G Don.

Plant cell culture technology, particularly suspension cultures, appears as a convenient tool to industrially produce molecules such as the anticancer molecules vincristine and vinblastine. Cell di ff erentiation is needed for their in vivo synthesis and thus in vitro cultures have usually been considered as limited producing platforms. Several studies have recently detected vincristine and vinblastine in early di ff erentiated calluses and also in suspension cultures. Nevertheless, the degree of cell di ff erentiation has not been addressed, particularly in suspension cultures that could be used as a large-scale producing platform. Therefore, the e ff ect of culture conditions on the production of vincristine and vinblastine, taking into account cytodi ff erentiation within cell aggregates, has been analyzed for the first time. Culture conditions such as light exposure and plant growth regulator regimes have been shown to a ff ect cell di ff erentiation. Moreover, cell di ff erentiation was observed to be closely related to vincristine and vinblastine titers. Results provide important clues into the comprehension of in vitro culture performance for metabolites production requiring in vivo cell di ff erentiation. They demonstrated the utility of taking into account cell di ff erentiation for the further development of novel advanced processes of di ff erentiated cell suspension cultures for producing valuable molecules, including biological medicines such as vincristine and vinblastine.