Characterisation of monoclonal antibodies to epizootic hemorrhagic disease virus of deer (EHDV) and bluetongue virus by immunisation of mice with EHDV recombinant VP7 antigen.

Immunisation of mice with recombinant VP7 antigen of epizootic hemorrhagic disease virus of deer (EHDV) induced serum antibody responses to EHDV. However, from the 19 monoclonal antibodies (Mab) produced from these mice, 15 were specific for EHDV and four for bluetongue virus (BTV). No Mabs were identified with the specificity for an epitope of VP7 shared by both EHDV and BTV in spite of the fact that they share a large portion of homology in VP7 amino acids composition. These Mabs were divided into five groups based on their specificity and interaction with each other. Group II Mabs, consisting of 13 Mabs, recognises a potential serogroup specific, linear epitope of EHDV VP7 antigen. One of the Mabs to BTV (Group V) was identified as BTV VP7 specific with the possibility of being the serogroup specific and recognizes a potential conformational epitope. Two Mabs from these VP7 specific groups were further analysed and found to be useful in a competitive enzyme-linked immunosorbent assay (C - ELISA) for detection of specific antibodies against EHDV and BTV in bovine sera.

[1]  J. Pearson,et al.  Evaluation of a Competitive ELISA for Detection of Antibodies to Epizootic Hemorrhagic Disease Virus of Deer , 1997, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[2]  M. Chan,et al.  Immunoblot Assay as a Confirmatory Test of Competitive ELISA for Serodiagnosis of Bluetongue , 1996, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[3]  J. Burroughs,et al.  Monoclonal antibody based competitive ELISA for the detection of antibodies against epizootic haemorrhagic disease of deer virus. , 1996, Journal of virological methods.

[4]  E. Zhou,et al.  Evidence That Two Distinct Populations of Rabbit Anti‐Idiotypic Antibodies Are Induced by Three Monoclonal Antibodies Specific for Bluetongue Virus Core Protein VP7 , 1996, Microbiology and immunology.

[5]  G. Dulac,et al.  Evaluation of a Commercial Competitive ELISA Test Kit for the Detection of Group-Specific Antibodies to Bluetongue Virus , 1993, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[6]  P. Wright,et al.  Development and evaluation of an enzyme-linked immunosorbent assay for detection of bovine antibodies to epizootic hemorrhagic disease of deer viruses. , 1992, Canadian journal of veterinary research = Revue canadienne de recherche veterinaire.

[7]  H. Iwata,et al.  Characterization of the genes encoding two of the major capsid proteins of epizootic haemorrhagic disease virus indicates a close genetic relationship to bluetongue virus. , 1992, The Journal of general virology.

[8]  J. White,et al.  A monoclonal antibody blocking ELISA detects antibodies specific for epizootic haemorrhagic disease virus. , 1991, Veterinary microbiology.

[9]  P. Roy,et al.  Synthesis and characterization of chimeric particles between epizootic hemorrhagic disease virus and bluetongue virus: functional domains are conserved on the VP3 protein , 1991, Journal of virology.

[10]  H. Iwata,et al.  Complete nucleotide sequence of segment 5 of epizootic haemorrhagic disease virus; the outer capsid protein VP5 is homologous to the VP5 protein of bluetongue virus. , 1991, Virus research.

[11]  A. Afshar,et al.  Comparison of competitive ELISA, indirect ELISA and standard AGID tests for detecting blue-tongue virus antibodies in cattle and sheep , 1989, Veterinary Record.

[12]  P. Wright,et al.  Comparison of competitive and indirect enzyme-linked immunosorbent assays for detection of bluetongue virus antibodies in serum and whole blood , 1987, Journal of clinical microbiology.

[13]  J. Appleton,et al.  Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. , 1983, Virology.

[14]  B. Erasmus,et al.  Identification of the serotype-specific and group-specific antigens of bluetongue virus. , 1981, The Onderstepoort journal of veterinary research.

[15]  E. Zhou,et al.  Anti-Idiotype to Bluetongue Virus VP7 Antigen: Potential Diagnostic Reagent and Vaccine , 1997 .

[16]  B. M. Gorman.  Bluetongue viruses. , 1990, Current topics in microbiology and immunology.

[17]  I M Parsonson,et al.  Pathology and pathogenesis of bluetongue infections. , 1990, Current topics in microbiology and immunology.