The influence of growth regulators concentrations on in vitro micropropagation of Ribes rubrum species.

In vitro cultures have found in a short time many practical applications, including genetic improvement of crop species, the multiplication of valuable genotypes, free virus propagation of existent vegetal stocks and conservation of genetic resources. In the present study we aimed to undertake a basic and simple protocol for in vitro micropropagation of Ribes rubrum considering the influence of various concentrations of growth regulators. There were used six variants (M1 ÷ M6) of half-strength MS medium supplemented with growth regulators, as follows: M1 (BA 0.2 mg/l and IBA 0.01 mg/), M2 (BA 0.4 mg/l, IBA 0.02 mg/l and GA3 0.1 mg/l), M3 (BA 0.4 mg/l, IBA 0.02 mg/ and GA3 0.2 mg/l), M4 (BA 0.15 mg/l, IBA 0.015 mg/l and GA3 0.15 mg/l), M5 (BA 0.5 mg/l) and M6 (BA 1 mg/l). The best medium for obtaining micro shoots was half-strength M3 medium. Therefore, the highest rate of in vitro shoot proliferation and elongation was obtained by using combination BA/IBA/GA3 as growth regulators.

[1]  F. Paprštein,et al.  In vitro establishment and proliferation of red currant cultivars , 2012 .

[2]  N. Khalid,et al.  Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan , 2011 .

[3]  W. Litwińczuk,et al.  Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid , 2009 .

[4]  Ansar Ali,et al.  Effect of different media and growth regulators on in vitro shoot proliferation of olive cultivar 'Moraiolo'. , 2009 .

[5]  Byoung Ryong Jeong,et al.  Influence of plant growth regulators on axillary shoot multiplication and iron source on growth of Scrophularia takesimensis Nakai - a rare endemic medicinal plant , 2008 .

[6]  M. Khouja,et al.  Effect of growth regulators and explant origin on in vitro propagation of Ceratonia siliqua L. via cuttings , 2008 .

[7]  D. Ružić,et al.  Micropropagation as Means of Rapid Multiplication of Newly Developed Blackberry and Black Currant Cultivars , 2007 .

[8]  C. Finn,et al.  Improving In Vitro Plant Regeneration from Leaf and Petiole Explants of `Marion' Blackberry , 2004 .

[9]  S. Millam,et al.  An assessment of the in-vitro multiplication rates of fourteen black currant cultivars , 1989 .

[10]  J. Zatykó.  Polyembryony induced in vitro in fertilized ovules of red currant cultivars. , 1980 .

[11]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .