Clinical aspects of egg bioactive peptide research: a review

Extensive in vitro and, to a more limited extent, animal research have demonstrated the health potential of a wide set of egg protein hydrolysates; however, well-designed human intervention studies are scarce. Health claim assessment, however, sets high standards for characterisation of the bioactive peptides, investigation of the mode-of-action and convincing human intervention studies. In this review, we discuss the state-of-the-art and knowledge gaps related to egg-derived peptide bioavailability and bioactivity. In addition, we will formulate recommendations for appropriate design of future human intervention trials with egg-derived peptides.

[1]  P. Strappe,et al.  Functional peptides derived from rice bran proteins , 2019, Critical reviews in food science and nutrition.

[2]  K. Majumder,et al.  Food-Derived Bioactive Peptides in Human Health: Challenges and Opportunities , 2018, Nutrients.

[3]  M. Kiely,et al.  Effect of an egg ovalbumin-derived protein hydrolysate on blood pressure and cardiovascular risk in adults with a mildly elevated blood pressure: a randomized placebo-controlled crossover trial , 2018, European Journal of Nutrition.

[4]  Jianping Wu,et al.  Egg and Soy-Derived Peptides and Hydrolysates: A Review of Their Physiological Actions against Diabetes and Obesity , 2018, Nutrients.

[5]  D. Ahn,et al.  Antioxidant, angiotensin-converting enzyme inhibitory activity and other functional properties of egg white proteins and their derived peptides - A review. , 2018, Poultry science.

[6]  Jianping Wu,et al.  Egg White Ovotransferrin Shows Osteogenic Activity in Osteoblast Cells. , 2018, Journal of Agricultural and Food Chemistry.

[7]  T. Matsui,et al.  Current knowledge of intestinal absorption of bioactive peptides. , 2017, Food & function.

[8]  J. Van Camp,et al.  Egg-derived bioactive peptides with ACE-inhibitory properties: a literature update. , 2017, Food & function.

[9]  Chibuike C. Udenigwe,et al.  Revisiting the mechanisms of ACE inhibitory peptides from food proteins , 2017 .

[10]  B. Hernández-Ledesma,et al.  Chemopreventive role of food-derived proteins and peptides: A review , 2017, Critical reviews in food science and nutrition.

[11]  Xiaodong Xie,et al.  Effect of statins on blood pressure: Analysis on adverse events released by FDA , 2017, Clinical and experimental hypertension.

[12]  R. Mensink,et al.  Effects of NWT-03, an egg-protein hydrolysate, on blood pressure in normotensive, high-normotensive and mild-hypertensive men and women: a dose-finding study. , 2017, British Journal of Nutrition.

[13]  H. van Loveren,et al.  Scientific and technical guidance for the preparation and presentation of a health claim application (Revision 2) , 2017, EFSA journal. European Food Safety Authority.

[14]  Sunil Kumar,et al.  Antihypertensive peptides of animal origin: A review , 2016, Critical reviews in food science and nutrition.

[15]  M. Kiely,et al.  Guidance for the design and implementation of human dietary intervention studies for health claim submissions , 2016 .

[16]  Q. Shen,et al.  Recent Research in Antihypertensive Activity of Food Protein-derived Hydrolyzates and Peptides , 2016, Critical reviews in food science and nutrition.

[17]  R. López-Fandiño,et al.  Pepsin Egg White Hydrolysate Ameliorates Obesity-Related Oxidative Stress, Inflammation and Steatosis in Zucker Fatty Rats , 2016, PloS one.

[18]  A. Zambrowicz,et al.  Pro-Cognitive Properties of the Immunomodulatory Polypeptide Complex, Yolkin, from Chicken Egg Yolk and Colostrum-Derived Substances: Analyses Based on Animal Model of Age-Related Cognitive Deficits , 2016, Archivum Immunologiae et Therapiae Experimentalis.

[19]  Jianping Wu,et al.  Bioactive peptides on endothelial function , 2016 .

[20]  Zhipeng Yu,et al.  Digestion and absorption of an egg white ACE-inhibitory peptide in human intestinal Caco-2 cell monolayers , 2016, International journal of food sciences and nutrition.

[21]  N. Howell,et al.  Antioxidant and ACE Inhibitory Bioactive Peptides Purified from Egg Yolk Proteins , 2015, International journal of molecular sciences.

[22]  Jingbo Liu,et al.  Transport of Antihypertensive Peptide RVPSL, Ovotransferrin 328-332, in Human Intestinal Caco-2 Cell Monolayers. , 2015, Journal of agricultural and food chemistry.

[23]  J. Buttriss.  Nutrition and health claims in practice , 2015 .

[24]  N. Deutz,et al.  Enhanced Lacto-Tri-Peptide Bio-Availability by Co-Ingestion of Macronutrients , 2015, PloS one.

[25]  Sunil Kumar,et al.  Bioactive peptides from egg: a review , 2015 .

[26]  S. Davidge,et al.  Egg-derived ACE-inhibitory peptides IQW and LKP reduce blood pressure in spontaneously hypertensive rats , 2015 .

[27]  D. I. Givens,et al.  Casein-Derived Lactotripeptides Reduce Systolic and Diastolic Blood Pressure in a Meta-Analysis of Randomised Clinical Trials , 2015, Nutrients.

[28]  Farooq Anwar,et al.  Recent advances in food biopeptides: production, biological functionalities and therapeutic applications. , 2015, Biotechnology advances.

[29]  S. Davidge,et al.  Beneficial Effects of Simulated Gastro-Intestinal Digests of Fried Egg and Its Fractions on Blood Pressure, Plasma Lipids and Oxidative Stress in Spontaneously Hypertensive Rats , 2014, PloS one.

[30]  A. Zambrowicz,et al.  Multifunctional peptides derived from an egg yolk protein hydrolysate: isolation and characterization , 2014, Amino Acids.

[31]  T. Matsuo,et al.  Effect of egg white and its hydrolysate on stearoyl-CoA desaturase index and fat accumulation in rat tissues , 2014, International journal of food sciences and nutrition.

[32]  Isidra Recio,et al.  Peptidomics for discovery, bioavailability and monitoring of dairy bioactive peptides , 2014 .

[33]  T. Matsuo,et al.  Increased muscular triglyceride content and hyperglycemia in Goto-Kakizaki rat are decreased by egg white hydrolysate , 2014, International journal of food sciences and nutrition.

[34]  Yujie Su,et al.  Interactions between tea polyphenol and two kinds of typical egg white proteins—ovalbumin and lysozyme: Effect on the gastrointestinal digestion of both proteins in vitro , 2014 .

[35]  P. Ferranti,et al.  Protein digestomics: Integrated platforms to study food-protein digestion and derived functional and active peptides , 2013 .

[36]  D. I. Givens,et al.  The impact of milk proteins and peptides on blood pressure and vascular function: a review of evidence from human intervention studies , 2013, Nutrition Research Reviews.

[37]  C. K. Yao,et al.  Design of Clinical Trials Evaluating Dietary Interventions in Patients With Functional Gastrointestinal Disorders , 2013, The American Journal of Gastroenterology.

[38]  Jianping Wu,et al.  Transport of IRW, an ovotransferrin-derived antihypertensive peptide, in human intestinal epithelial Caco-2 cells. , 2013, Journal of agricultural and food chemistry.

[39]  C. Borghi,et al.  Do the Lactotripeptides Isoleucine–Proline–Proline and Valine–Proline–Proline Reduce Systolic Blood Pressure in European Subjects? A Meta-Analysis of Randomized Controlled Trials , 2013, American journal of hypertension.

[40]  E. Strocchi,et al.  Predictors of the Short-Term Effect of Isoleucine–Proline–Proline/Valine–Proline–Proline Lactotripeptides from Casein on Office and Ambulatory Blood Pressure in Subjects with Pharmacologically Untreated High-Normal Blood Pressure or First-Degree Hypertension , 2012, Clinical and experimental hypertension.

[41]  W. Kraemer,et al.  Acute effects of ingestion of a novel whey-derived extract on vascular endothelial function in overweight, middle-aged men and women , 2012, British Journal of Nutrition.

[42]  Efsa Panel on Dietetic Products.  Scientific Opinion on the substantiation of health claims related to isoleucine‐proline‐proline (IPP) and valine‐proline‐proline (VPP) and maintenance of normal blood pressure (ID 661, 1831, 1832, 2891, further assessment) pursuant to Article 13(1) of Regulation (EC) No 1924/2006 , 2012 .

[43]  Fei Wang,et al.  Characterization of ACE-inhibitory peptide associated with antioxidant and anticoagulation properties. , 2011, Journal of food science.

[44]  E. Strocchi,et al.  Lactotripeptides effect on office and 24-h ambulatory blood pressure, blood pressure stress response, pulse wave velocity and cardiac output in patients with high-normal blood pressure or first-degree hypertension: a randomized double-blind clinical trial , 2011, Hypertension Research.

[45]  X. Yue,et al.  Optimization of Hydrolysis Pretreatment Conditions with Two Kinds of Enzymes for Enhanced Proteolysis in Egg Yolk Solution Using Response Surface Methodology , 2011 .

[46]  L. Laghi,et al.  Blood pressure lowering effect of lactotripeptides assumed as functional foods: a meta-analysis of current available clinical trials , 2011, Journal of Human Hypertension.

[47]  K. Leem,et al.  Protective effect of egg yolk peptide on bone metabolism , 2011, Menopause.

[48]  C. Melegari,et al.  Hemodynamic effects of lactotripeptides from casein hydrolysate in Mediterranean normotensive subjects and patients with high-normal blood pressure: a randomized, double-blind, crossover clinical trial. , 2010, Journal of medicinal food.

[49]  R. Marchelli,et al.  Scientific Opinion on the substantiation of health claims related to bonito protein peptide and maintenance of normal blood pressure (ID 1716) pursuant to Article 13(1) of Regulation (EC) No 1924/2006 , 2010 .

[50]  Jianping Wu,et al.  A new approach for identification of novel antihypertensive peptides from egg proteins by QSAR and bioinformatics , 2010 .

[51]  W. Kraemer,et al.  Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial , 2009, Nutrition journal.

[52]  R. Draijer,et al.  Lactotripeptides do not lower ambulatory blood pressure in untreated whites: results from 2 controlled multicenter crossover studies. , 2009, The American journal of clinical nutrition.

[53]  Jianping Wu,et al.  Angiotensin I converting enzyme inhibitory peptides from simulated in vitro gastrointestinal digestion of cooked eggs. , 2009, Journal of agricultural and food chemistry.

[54]  M. A. Lasunción,et al.  Transepithelial transport across Caco-2 cell monolayers of antihypertensive egg-derived peptides. PepT1-mediated flux of Tyr-Pro-Ile. , 2008, Molecular nutrition & food research.

[55]  R. López-Fandiño,et al.  Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats. , 2008, Food chemistry.

[56]  R. López-Fandiño,et al.  Angiotensin-converting enzyme activity in plasma and tissues of spontaneously hypertensive rats after the short- and long-term intake of hydrolysed egg white. , 2007, Molecular nutrition & food research.

[57]  Chien-Ning Huang,et al.  Bovine casein hydrolysate (c12 Peptide) reduces blood pressure in prehypertensive subjects. , 2007, American journal of hypertension.

[58]  R. López-Fandiño,et al.  Long-term intake of egg white hydrolysate attenuates the development of hypertension in spontaneously hypertensive rats. , 2006, Life sciences.

[59]  R. López-Fandiño,et al.  Effect of simulated gastrointestinal digestion on the antihypertensive properties of ACE-inhibitory peptides derived from ovalbumin. , 2006, Journal of agricultural and food chemistry.

[60]  R. López-Fandiño,et al.  Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats , 2005, British Journal of Nutrition.

[61]  Kevin A Cockell,et al.  Effects of antinutritional factors on protein digestibility and amino acid availability in foods. , 2005, Journal of AOAC International.

[62]  R. López-Fandiño,et al.  Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. , 2004, Journal of food protection.

[63]  Kenji Watanabe,et al.  Egg ovomucin attenuates hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells , 2002, Lipids.

[64]  S. Arai,et al.  Transepithelial Transport of Oligopeptides in the Human Intestinal Cell, Caco-2 , 1997, Peptides.