The sewing laboratory is a space used by students almost every semester throughout the fashion course degree. It is common for students to staying in the laboratory for several hours nonstop, which justifies the importance of carrying out a study on this environment in order to identify issues. Therefore, the objective of this paper is to perform a macroergonomic analysis in the sewing laboratory of the bachelor fashion design program at the UDESC - Santa Catarina State University (Florianopolis, Brazil), in order to identify the Ergonomic Demand Items through the Work Macroergonomics Analysis (AMT) taking into consideration the Human Factors involved in the activities practiced in this workspace. The methodological procedures involved the Work Macroergonomics Analysis method, proposed by Fogliatto and Guimaraes (1998). As a result, the macroergonomic approach was considered efficient in the identification of ergonomic demands, which help in the evaluation and propositions of suggestions for improvement the sewing laboratory, increasing the efficiency and effectiveness of the practices performed there, as well as health and wellbeing of the students, technicians and teachers who use this workspace.