Detection of Herpes Simplex Virus and Human Papilloma Virus in Ophthalmic Pterygium

Purpose. To evaluate the presence of herpes simplex virus (HSV) and human papilloma virus (HPV) in pterygia and phenotypically normal conjunctiva and the possible relation between viral presence and clinical information. Methods. Fifty pterygia and respective conjunctival specimens were obtained. A personal and family history was recorded for each patient. HSV and HPV detection and typing were accomplished by polymerase chain reaction amplification of viral sequences. Results were statistically analyzed. Results. HSV (type 1) was detected in 11 (22%), HPV (type 18) in 12 (24%), and both HSV-1 and HPV-18 in 3 (6%) of pterygia. No conjunctival specimen displayed HSV, whereas HPV was detected in four (8%). Postoperative recurrence and history of conjunctivitis were significantly more common in patients with simultaneous detection of HSV and HPV. Conclusion. The fact that HSV was not detected in conjunctival specimens implies a more specific correlation with pterygium, as compared with HPV. The detection of potentially oncogenic viruses, such as HSV and HPV, supports the concept that pterygium can be considered a neoplastic condition. The correlation of postoperative recurrence and a history of conjunctivitis with the simultaneous detection of HPV and HSV, implies a possible viral cooperation affecting the clinical profile of pterygium.

[1]  B. Rogers,et al.  Detection of herpes simplex virus using the polymerase chain reaction followed by endonuclease cleavage. , 1991, The American journal of pathology.

[2]  T. D. Duane,et al.  Duane's Clinical Ophthalmology , 1993 .

[3]  A. Nesburn,et al.  Latent herpes simplex virus from trigeminal ganglia of rabbits with recurrent eye infection. , 1972, Nature: New biology.

[4]  J. Rosborough,et al.  In vivo reactivation of herpes simplex virus in rabbit trigeminal ganglia: electrode model , 1981, Infection and immunity.

[5]  H. zur Hausen,et al.  Papillomavirus infections--a major cause of human cancers. , 1996, Biochimica et biophysica acta.

[6]  S. Love,et al.  Autopsy findings in two cases of neonatal herpes simplex virus infection: detection of virus by immunohistochemistry, in situ hybridization and the polymerase chain reaction , 1994, Histopathology.

[7]  J. Malter,et al.  Human papillomavirus type 18 in conjunctival intraepithelial neoplasia. , 1990, American journal of ophthalmology.

[8]  D. Galloway,et al.  Alterations in the cellular phenotype induced by herpes simplex viruses , 1990, Journal of medical virology.

[9]  F. Doran,et al.  Human papillomavirus in pterygium. , 1994, The Central African journal of medicine.

[10]  G. R. Taylor,et al.  PCR: a practical approach. , 1991 .

[11]  N. Maitland,et al.  Human herpes simplex-1 and papillomavirus type 16 homologous DNA sequences in normal, potentially malignant and malignant oral mucosa. , 1993, European journal of cancer. Part B, Oral oncology.

[12]  P. Nucci,et al.  Unexpected histological findings in pterygium. , 1993, Optometry and Vision Science.

[13]  W. Martin,et al.  DNA of human papillomavirus type 16 in dysplastic and malignant lesions of the conjunctiva and cornea. , 1989, The New England journal of medicine.

[14]  M. Orhan,et al.  Tear functions in patients with pterygium. , 1998, Acta ophthalmologica Scandinavica.

[15]  G. Sourvinos,et al.  Microsatellite instability and loss of heterozygosity in human pterygia , 1997, The British journal of ophthalmology.

[16]  J H HILGERS,et al.  Pterygium: its incidence, heredity and etiology. , 1960, American journal of ophthalmology.

[17]  A. Wyllie,et al.  HPV in full thickness cervical biopsies: High prevalence in CIN 2 and CIN 3 detected by a sensitive PCR method , 1991, The Journal of pathology.

[18]  C. Jones,et al.  Characterization of primary human fibroblasts transformed by human papilloma virus type 16 and herpes simplex virus type 2 DNA sequences. , 1992, The Journal of general virology.

[19]  D. Spandidos,et al.  Evaluation of loss of heterozygosity and microsatellite instability in human pterygium: clinical correlations , 1998, The British journal of ophthalmology.

[20]  H. E. Kaufman,et al.  Herpes simplex keratitis. , 1983, Ophthalmology.

[21]  P. Finger,et al.  Scleral hyperplasia induced by heat. , 1986, American journal of ophthalmology.

[22]  P. Thygeson,et al.  Ocular viral diseases. , 1959, The Medical clinics of North America.

[23]  R. Wallace.  Is this a practical approach? , 2001, Journal of the American College of Surgeons.

[24]  G. Snow,et al.  The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. , 1990, The Journal of general virology.

[25]  W. Mitchell,et al.  Persistence of Herpes Simplex Virus Type 1 DNA in Chronic Conjunctival and Eyelid Lesions of Mice , 1998, Journal of Virology.

[26]  M. Coroneo.  Pterygium as an early indicator of ultraviolet insolation: a hypothesis. , 1993, The British journal of ophthalmology.

[27]  O D Pinkerton,et al.  Immunologic basis for the pathogenesis of pterygium. , 1984, American journal of ophthalmology.

[28]  K. Bean,et al.  Detection of HSV nucleic acid sequences in the cornea during acute and latent ocular disease. , 1988, Experimental eye research.

[29]  T. Hill,et al.  AN ALTERNATIVE THEORY OF HERPES-SIMPLEX RECURRENCE AND A POSSIBLE ROLE FOR PROSTAGLANDINS , 1976, The Lancet.

[30]  M. Boon,et al.  Detection of the BC 24 transforming fragment of the herpes simplex virus type 2 (HSV‐2) DNA in cervical carcinoma tissue by polymerase chain reaction (PCR) , 1994, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[31]  N. Park,et al.  In vitro and animal studies of the role of viruses in oral carcinogenesis. , 1992, European journal of cancer. Part B, Oral oncology.

[32]  P. McDonnell,et al.  Human papillomavirus DNA in a recurrent squamous carcinoma of the eyelid. , 1989, Archives of ophthalmology.