Cloning and molecular characterization of an ovarian-derived (brain-like) P450 aromatase cDNA and development of a competitive RT-PCR assay to quantify its expression in the fathead minnow (Pimephales promelas)

[1]  Yann Guiguen,et al.  Involvement of estrogens in the process of sex differentiation in two fish species: The rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus) , 1999, Molecular reproduction and development.

[2]  S. Abe,et al.  Suppression of P450 aromatase gene expression in sex-reversed males produced by rearing genetically female larvae at a high water temperature during a period of sex differentiation in the Japanese flounder (Paralichthys olivaceus). , 1999, Journal of molecular endocrinology.

[3]  D. Boerboom,et al.  Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching1. , 1999, Endocrinology.

[4]  W. Boon,et al.  Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[5]  D. Boerboom,et al.  Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts in equine granulosa cells during human chorionic gonadotropin-induced ovulation: a novel model for the study of aromatase promoter switching. , 1999, Endocrinology.

[6]  P. Oefner,et al.  Modeling and analysis of competitive RT-PCR. , 1998, Nucleic acids research.

[7]  G. Callard,et al.  Printed in U.S.A. Copyright © 1998 by The Endocrine Society Identification of Multiple CYP19 Genes Encoding , 2022 .

[8]  D. Gelinas,et al.  Isolation of a goldfish brain cytochrome P450 aromatase cDNA: mRNA expression during the seasonal cycle and after steroid treatment 1 The nucleotide sequence reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number U18974. 1 , 1998, Molecular and Cellular Endocrinology.

[9]  竹森 洋.  Polyadenylation-mediated translational regulation of maternal P450 (11β) mRNA in frog oocytes , 1998 .

[10]  J. Sumpter,et al.  Endocrine disruption in wildlife: a critical review of the evidence. , 1998, Critical reviews in toxicology.

[11]  M. Okamoto,et al.  Polyadenylation-Mediated Translational Regulation of Maternal P450(llβ) mRNA in Frog Oocytes , 1997 .

[12]  J. Trant,et al.  Expression of cytochrome P450 aromatase in the channel catfish, Ictalurus punctatus , 1997, The Journal of Steroid Biochemistry and Molecular Biology.

[13]  Tohru Kobayashi,et al.  Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis. , 1997, Journal of molecular endocrinology.

[14]  S. Bulun,et al.  Expression of the CYP19 (aromatase) gene: an unusual case of alternative promoter usage , 1997, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[15]  B. White.  PCR cloning protocols : from molecular cloning to genetic engineering , 1997 .

[16]  K. Roux,et al.  One-step optimization using touchdown and stepdown PCR. , 1997, Methods in molecular biology.

[17]  J. Vanselow,et al.  Tissue-specific expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative first exons. , 1997, Endocrinology.

[18]  Minoru Tanaka,et al.  Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P‐450 aromatase , 1996, Molecular reproduction and development.

[19]  M. Wickens,et al.  Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[20]  C. Choong,et al.  An evaluation of competitor type and size for use in the determination of mRNA by competitive PCR. , 1995, PCR methods and applications.

[21]  R. Devlin,et al.  Brief treatment with an aromatase inhibitor during sex differentiation causes chromosomally female salmon to develop as normal, functional males , 1994 .

[22]  J. Trant.  Isolation and characterization of the cDNA encoding the channel catfish (Ictalurus punctatus) form of cytochrome P450arom. , 1994, General and comparative endocrinology.

[23]  Y Nagahama,et al.  Endocrine regulation of gametogenesis in fish. , 1994, The International journal of developmental biology.

[24]  D. Gelinas,et al.  Immunocytochemical and Biochemical Evidence for Aromatase in Neurons of the Retina, Optic Tectum and Retinotectal Pathways in Goldfish , 1993, Journal of neuroendocrinology.

[25]  B. Rouse,et al.  Limitations and modifications of quantitative polymerase chain reaction. Application to measurement of multiple mRNAs present in small amounts of sample RNA. , 1993, Journal of immunological methods.

[26]  M. Zenilman,et al.  A rapid and versatile method to synthesize internal standards for competitive PCR. , 1993, Nucleic acids research.

[27]  Minoru Tanaka,et al.  Cloning and sequence analysis of the cDNA encoding P-450 aromatase (P450arom) from a rainbow trout (Oncorhynchus mykiss) ovary; relationship between the amount of P450arom mRNA and the production of oestradiol-17β in the ovary , 1992 .

[28]  C. Mendelson,et al.  Structure-function relationships of human aromatase cytochrome P-450 using molecular modeling and site-directed mutagenesis. , 1991, The Journal of biological chemistry.

[29]  J. Richards,et al.  Aromatase cytochrome P450 in rat ovarian granulosa cells before and after luteinization: adenosine 3',5'-monophosphate-dependent and independent regulation. Cloning and sequencing of rat aromatase cDNA and 5' genomic DNA. , 1990, Molecular endocrinology.

[30]  J. Wilson,et al.  The expression of a functional cDNA encoding the chicken cytochrome P-450arom (aromatase) that catalyzes the formation of estrogen from androgen. , 1988, The Journal of biological chemistry.

[31]  G. Callard,et al.  Changes in brain aromatase and 5 alpha-reductase activities correlate significantly with seasonal reproductive cycles in goldfish (Carassius auratus). , 1988, Endocrinology.

[32]  T. Mohandas,et al.  Human aromatase: cDNA cloning, Southern blot analysis, and assignment of the gene to chromosome 15. , 1988, DNA.

[33]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[34]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[35]  M. Kozak.  Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes , 1986, Cell.