Complete cDNA sequence, expression, alternative splicing, and genomic organization of the mouse Nfat5 gene

Members of the NFAT (nuclear factors of activated T cells) gene family have been investigated in numerous organisms, including man and mouse. All NFATs may be synthesized in several isoforms differing in amino or carboxy termini due to 5′ and 3′ alternative splicing of the corresponding mRNA. Recently, we mapped the murine Nfat5 gene to chro- mosome 8D. In the present paper we describe for the first time the complete sequence and primary structure of murine Nfat5, two new spliced isoforms, and the expression of murine Nfat5 in embryonic and adult mouse tissues.

[1]  E. Schwinger,et al.  Genomic organization of the human NFAT5 gene: exon-intron structure of the 14-kb transcript and CpG-island analysis of the promoter region , 2001, Cytogenetic and Genome Research.

[2]  E. Schwinger,et al.  Quantitative PCR analysis of different splice forms of NFAT5 revealed specific gene expression in fetal and adult brain. , 2000, Brain research. Molecular brain research.

[3]  S. Ho,et al.  The NFAT-Related Protein NFATL1 (TonEBP/NFAT5) Is Induced Upon T Cell Activation in a Calcineurin-Dependent Manner1 , 2000, The Journal of Immunology.

[4]  M. Mattei,et al.  Assignment of transcription factor NFAT5 to human chromosome 16q22.1, murine chromosome 8D and porcine chromosome 6p1.4 and comparison of the polyglutamine domains , 2000, Cytogenetic and Genome Research.

[5]  K. Arai,et al.  NFATz: a novel rel similarity domain containing protein. , 2000, Biochemical and biophysical research communications.

[6]  A. Rao,et al.  NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Crabtree.  Contingent genetic regulatory events in T lymphocyte activation. , 1989, Science.

[8]  N. Copeland,et al.  NF-AT5: the NF-AT family of transcription factors expands in a new direction. , 1999, Cold Spring Harbor symposia on quantitative biology.

[9]  M. Suyama,et al.  Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. , 1998, DNA research : an international journal for rapid publication of reports on genes and genomes.

[10]  H. Mocharla,et al.  Coupled reverse transcription-polymerase chain reaction (RT-PCR) as a sensitive and rapid method for isozyme genotyping. , 1990, Gene.