Repair of X-ray-induced DNA damage in rat cerebellar neurons and brain tumor cells.

The in vivo DNA repair kinetics of rat cerebellar neurons and intracerebral 9L tumor cells were simultaneously assessed in the same rat after a single 5000-rad dose of X rays using an alkaline sucrose gradient sedimentation technique in slow reorienting zonal rotors. An X-ray dose of 5000 rad produced the same amount of DNA damage in both 9L tumor cells and cerebellar neurons. However, at every repair time studied up to 24 hr, the tumor cell DNA was always repaired to a greater extent than the neuronal DNA. Restoration of the chromosomal DNA structure to its unirradiated state in intracerebral 9L tumor cells and in cerebellar neurons required 24 and 38 hr, respectively. Thus, nondividing neurons appear to repair their radiation-induced DNA damage to the same extent as the dividing tumor cells, but they do so at a slower rate.

[1]  B. Rydberg,et al.  Radiation-induced DNA strand breaks and their rejoining in crypt and villous cells of the small intestine of the mouse. , 1975, Radiation research.

[2]  J. Leith,et al.  Cellular radiosensitivity of a rat brain tumor , 1975, Cancer.

[3]  K. Wheeler,et al.  Characterization of large mammalina DNA species sedimented in a reorienting zonal rotor. , 1975, Analytical biochemistry.

[4]  S. Okada.  Repair Studies at the Molecular, Chromosomal, and Cellular Levels: a Review of Current Work in Japan1 , 1975 .

[5]  J. Lett,et al.  On the possibility that DNA repair is related to age in non-dividing cells. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[6]  B. Zimm.  Anomalies in sedimentation: IV. Decrease in sedimentation coefficients of chains at high fields , 1974 .

[7]  J. Lett,et al.  A marker for mammalian DNA sedimentation. , 1974, Radiation research.

[8]  M. Rajewsky,et al.  Persistence of O6-ethylguanine in rat-brain DNA: correlation with nervous system-specific carcinogenesis by ethylnitrosourea. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[9]  T. Ono,et al.  Estimation in vivo of DNA strand breaks and their rejoining in thymus and liver of mouse. , 1974, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[10]  I. Damjanov,et al.  Patterns of damage and repair of liver DNA induced by carcinogenic methylating agents in vivo. , 1973, Cancer research.

[11]  C. Wilson,et al.  Development of an animal brain tumor model and its response to therapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. , 1973, Cancer research.

[12]  J. Lett,et al.  In vivo restitution of the DNA structure in gamma irradiated rabbit retinas. , 1973, Radiation research.

[13]  P. Karran,et al.  Is the ability to repair damage to DNA related to the proliferative capacity of a cell? The rejoining of X-ray-produced strand breaks. , 1973, Biochimica et biophysica acta.

[14]  J. Lett,et al.  Formation and rejoining of DNA strand breaks in irradiated neurons: in vivo. , 1972, Radiation research.

[15]  T. Makinodan,et al.  Age-Associated Changes in the DNA of Mouse Tissue , 1971, Science.

[16]  A. Schiller,et al.  Morphological studies of rat brain tumors induced by N-nitrosomethylurea. , 1971, Journal of neurosurgery.

[17]  H. Curtis,et al.  Chromosome aberrations in liver cells of dogs of different ages. , 1966, Journal of gerontology.