Surface presentation of protein epitopes using bacteriophage expression systems.

Vast libraries of filamentous phage expression vectors that display foreign (poly)peptides on the virion surface can be screened by affinity-purifying those phage whose displayed foreign peptide binds to an antibody or another binding protein. Present libraries display only short random peptides, but work is presently underway to create libraries displaying antibodies with a great diversity of binding specificities.

[1]  J. Scott,et al.  Searching for peptide ligands with an epitope library. , 1990, Science.

[2]  G. P. Smith,et al.  Antibody-selectable filamentous fd phage vectors: affinity purification of target genes. , 1988, Gene.

[3]  J. Devlin,et al.  Random peptide libraries: a source of specific protein binding molecules. , 1990, Science.

[4]  G. P. Smith,et al.  Filamentous phages as cloning vectors. , 1988, Biotechnology.

[5]  B. Seed,et al.  Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[6]  G. Winter,et al.  Phage antibodies: filamentous phage displaying antibody variable domains , 1990, Nature.

[7]  C. Barbas,et al.  Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D R Burton,et al.  Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. , 1989, Science.

[9]  R. Barrett,et al.  Peptides on phage: a vast library of peptides for identifying ligands. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[10]  M. Hofnung,et al.  Versatility of a vector for expressing foreign polypeptides at the surface of gram-negative bacteria. , 1988, Gene.

[11]  G. P. Smith,et al.  Filamentous phage assembly: morphogenetically defective mutants that do not kill the host. , 1988, Virology.

[12]  D. Mead,et al.  Chimeric single-stranded DNA phage-plasmid cloning vectors. , 1988, Biotechnology.

[13]  G. P. Smith,et al.  Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. , 1985, Science.

[14]  J. Wells,et al.  Hormone phage: An enrichment method for variant proteins with altered binding properties , 1990, Proteins.

[15]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[16]  J. Tommassen,et al.  Outer-membrane PhoE protein of Escherichia coli K-12 as an exposure vector: possibilities and limitations. , 1990, Gene.

[17]  G. Cesareni.  Phage-plasmid hybrid vectors. , 1988, Biotechnology.

[18]  R. Perham,et al.  Multiple display of foreign peptides on a filamentous bacteriophage. Peptides from Plasmodium falciparum circumsporozoite protein as antigens. , 1991, Journal of molecular biology.

[19]  M. Little,et al.  A surface expression vector for antibody screening. , 1991, Gene.