Application of a Pyruvate-Producing Escherichia coli Strain LAFCPCPt-accBC-aceE: A Case Study for d-Lactate Production

Pyruvate, a potential precursor of various chemicals, is one of the fundamental chemicals produced by the fermentation process. We previously reported a pyruvate-producing Escherichia coli strain LAFCPCPt-accBC-aceE (PYR) that has the potential to be applied to the industrial production of pyruvate. In this study, the availability of the PYR strain for the production of pyruvate-derivative chemicals was evaluated using a d-lactate-producing strain (LAC) based on the PYR strain. The LAC strain expresses a d-lactate dehydrogenase-encoding gene from Lactobacillus bulgaricus under the control of a T7 expression system. The d-lactate productivity of the LAC strain was further improved by limiting aeration and changing the induction period for the expression of d-lactate dehydrogenase-encoding gene expression. Under combined conditions, the LAC strain produced d-lactate at 21.7 ± 1.4 g·L−1, which was compatible with the pyruvate production by the PYR strain (26.1 ± 0.9 g·L−1). These results suggest that we have succeeded in the effective conversion of pyruvate to d-lactate in the LAC strain, demonstrating the wide versatility of the parental PYR strain as basal strain for various chemicals production.

[1]  Jian Chen,et al.  Enhanced Pyruvate Production in Candida glabrata by Engineering ATP Futile Cycle System. , 2019, ACS synthetic biology.

[2]  Tao Chen,et al.  Evolutionary engineering of Escherichia coli for improved anaerobic growth in minimal medium accelerated lactate production , 2019, Applied Microbiology and Biotechnology.

[3]  Jae Sung Cho,et al.  A comprehensive metabolic map for production of bio-based chemicals , 2019, Nature Catalysis.

[4]  T. Tan,et al.  Cofactor engineering for more efficient production of chemicals and biofuels. , 2017, Biotechnology advances.

[5]  Hironaga Akita,et al.  Production of d-lactate using a pyruvate-producing Escherichia coli strain , 2017, Bioscience, biotechnology, and biochemistry.

[6]  M. Eiteman,et al.  Recent Progress in the Microbial Production of Pyruvic Acid , 2017 .

[7]  H. Shimizu,et al.  13C-metabolic flux analysis for mevalonate-producing strain of Escherichia coli. , 2017, Journal of bioscience and bioengineering.

[8]  Tamotsu Hoshino,et al.  Pyruvate production using engineered Escherichia coli , 2016, AMB Express.

[9]  Taku Nishimura,et al.  Efficient production and secretion of pyruvate from Halomonas sp. KM-1 under aerobic conditions , 2016, AMB Express.

[10]  T. Hanai,et al.  Metabolic flux redirection from a central metabolic pathway toward a synthetic pathway using a metabolic toggle switch. , 2014, Metabolic engineering.

[11]  H. Shimizu,et al.  A Vector Library for Silencing Central Carbon Metabolism Genes with Antisense RNAs in Escherichia coli , 2013, Applied and Environmental Microbiology.

[12]  N. Nakashima,et al.  Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline‐inducible vectors , 2013, Letters in applied microbiology.

[13]  S. Fukiya,et al.  Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes. , 2012, Journal of biotechnology.

[14]  M. Inui,et al.  Improvement of the Redox Balance Increases l-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions , 2011, Applied and Environmental Microbiology.

[15]  Guanjun Chen,et al.  Production of Lactate in Escherichia coli by Redox Regulation Genetically and Physiologically , 2011, Applied biochemistry and biotechnology.

[16]  H. Tegel,et al.  Enhancing the protein production levels in Escherichia coli with a strong promoter , 2011, The FEBS journal.

[17]  Wei Shen,et al.  Evaluation of Genetic Manipulation Strategies on d-Lactate Production by Escherichia coli , 2011, Current Microbiology.

[18]  Ping Xu,et al.  An efficient method for N-acetyl-d-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system , 2010, Applied Microbiology and Biotechnology.

[19]  J. Paniagua-Michel,et al.  The biosynthesis and accumulation of β-carotene in Dunaliella salina proceed via the glyceraldehyde 3-phosphate/pyruvate pathway , 2009, Natural product research.

[20]  Bernhard Ø. Palsson,et al.  Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain , 2008, Applied and Environmental Microbiology.

[21]  Ming Liu,et al.  Inactivation of aldehyde dehydrogenase: a key factor for engineering 1,3-propanediol production by Klebsiella pneumoniae. , 2006, Metabolic engineering.

[22]  K. Shanmugam,et al.  Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli , 2006, Biotechnology Letters.

[23]  Susan Jones.  In Brief , 2004, Nature Reviews Microbiology.

[24]  K. Shanmugam,et al.  Production of Optically Pure d-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 , 2003, Applied and Environmental Microbiology.

[25]  C. Khosla,et al.  Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli. , 2002, Biochemistry.

[26]  S. Lun,et al.  Biotechnological production of pyruvic acid , 2001, Applied Microbiology and Biotechnology.

[27]  Jae-Gu Pan,et al.  Homofermentative Production of d- orl-Lactate in Metabolically Engineered Escherichia coli RR1 , 1999, Applied and Environmental Microbiology.

[28]  H. Shimizu,et al.  Pyruvic acid production by an F1-ATPase-defective mutant of Escherichia coli W1485lip2. , 1994, Bioscience, biotechnology, and biochemistry.

[29]  D. Clark,et al.  The fermentation pathways of Escherichia coli. , 1989, FEMS microbiology reviews.