Domestication of wild tomato is accelerated by genome editing

Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR–Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.

[1]  E. van der Knaap,et al.  What lies beyond the eye: the molecular mechanisms regulating tomato fruit weight and shape , 2014, Front. Plant Sci..

[2]  Kabin Xie,et al.  CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants. , 2017, Molecular plant.

[3]  Z. Lippman,et al.  Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System1 , 2014, Plant Physiology.

[4]  Tao Huang,et al.  Genomic architecture of heterosis for yield traits in rice , 2016, Nature.

[5]  J. Qiu,et al.  Progress and prospects in plant genome editing , 2017, Nature Plants.

[6]  Kui Lin,et al.  Genomic analyses provide insights into the history of tomato breeding , 2014, Nature Genetics.

[7]  P. Sandøe,et al.  Accelerating the Domestication of New Crops: Feasibility and Approaches. , 2017, Trends in plant science.

[8]  S. Park,et al.  Optimization of crop productivity in tomato using induced mutations in the florigen pathway , 2014, Nature Genetics.

[9]  Eva Konečná,et al.  A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants[OPEN] , 2017, Plant Cell.

[10]  S. Tanksley,et al.  Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L. esculentum var. Giant Heirloom. , 2001, Genetics.

[11]  S. Park,et al.  Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato , 2016, Nature Genetics.

[12]  Z. Lippman,et al.  A cascade of arabinosyltransferases controls shoot meristem size in tomato , 2015, Nature Genetics.

[13]  E. S. Rao,et al.  Relationship between survival and yield related traits in Solanum pimpinellifolium under salt stress , 2012, Euphytica.

[14]  Zachary B. Lippman,et al.  Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing , 2017, Cell.

[15]  D. Voytas,et al.  Genome editing as a tool to achieve the crop ideotype and de novo domestication of wild relatives: Case study in tomato. , 2017, Plant science : an international journal of experimental plant biology.

[16]  Hui-Li Xing,et al.  A CRISPR/Cas9 toolkit for multiplex genome editing in plants , 2014, BMC Plant Biology.

[17]  M. Robbins,et al.  Characterization of hypersensitive resistance to bacterial spot race T3 (Xanthomonas perforans) from tomato accession PI 128216. , 2009, Phytopathology.

[18]  David Jackson,et al.  CLAVATA-WUSCHEL signaling in the shoot meristem , 2016, Development.

[19]  A. Walmsley,et al.  Tomato (Lycopersicum esculentum). , 2006, Methods in molecular biology.

[20]  Wencai Yang,et al.  Marker-assisted Selection for Combining Resistance to Bacterial Spot and Bacterial Speck in Tomato , 2005 .

[21]  Bruce D. Smith,et al.  The Molecular Genetics of Crop Domestication , 2006, Cell.

[22]  Yanpeng Wang,et al.  Genome editing in rice and wheat using the CRISPR/Cas system , 2014, Nature Protocols.

[23]  X. Ji,et al.  Genome editing of upstream open reading frames enables translational control in plants , 2018, Nature Biotechnology.

[24]  F. Nuez,et al.  Genetic and bioclimatic variation in Solanum pimpinellifolium , 2009, Genetic Resources and Crop Evolution.

[25]  J. Levin,et al.  The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE. , 1996, The Plant journal : for cell and molecular biology.

[26]  Rachel S. Meyer,et al.  Evolution of crop species: genetics of domestication and diversification , 2013, Nature Reviews Genetics.