CC chemokine receptors 1 and 3 are differentially regulated by IL-5 during maturation of eosinophilic HL-60 cells.

CC chemokine receptors 1 and 3 (CCR1 and CCR3) are expressed by eosinophils; however, factors regulating their expression and function have not previously been defined. Here we analyze chemokine receptor expression and function during eosinophil differentiation, using the eosinophilic cell line HL-60 clone 15 as a model system. RNA for CCR1, -3, -4, and -5 was not detectable in the parental cells, and the cells did not specifically bind CC chemokines. Cells treated with butyric acid acquired eosinophil characteristics; expressed mRNA for CCR1 and CCR3, but not for CCR4 or CCR5; acquired specific binding sites for macrophage-inflammatory protein-1alpha and eotaxin (the selective ligands for CCR1 and CCR3, respectively); and exhibited specific calcium flux and chemotaxis responses to macrophage-inflammatory protein-1alpha, eotaxin, and other known CCR1 and CCR3 agonists. CCR3 was expressed later and at lower levels than CCR1 and could be further induced by IL-5, whereas IL-5 had little or no effect on CCR1 expression. Consistent with the HIV-1 coreceptor activity of CCR3, HL-60 clone 15 cells induced with butyric acid and IL-5 fused with HeLa cells expressing CCR3-tropic HIV-1 envelope glycoproteins, and fusion was blocked specifically by eotaxin or an anti-CCR3 mAb. These data suggest that CCR1 and CCR3 are markers of late eosinophil differentiation that are differentially regulated by IL-5 in this model.

[1]  P. Murphy,et al.  Determinants of HIV-1 Coreceptor Function on CC Chemokine Receptor 3 , 1997, The Journal of Biological Chemistry.

[2]  P. Simmonds,et al.  HIV-1 tropism and co-receptor use , 1997, Nature.

[3]  J. Sodroski,et al.  CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia , 1997, Nature.

[4]  C. Mackay,et al.  Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody. , 1997, The Journal of clinical investigation.

[5]  C. Broder,et al.  Cell type-specific fusion cofactors determine human immunodeficiency virus type 1 tropism for T-cell lines versus primary macrophages , 1996, Journal of virology.

[6]  M. Baggiolini,et al.  Interleukin-2 regulates CC chemokine receptor expression and chemotactic responsiveness in T lymphocytes , 1996, The Journal of experimental medicine.

[7]  P. Gray,et al.  Molecular Cloning and Functional Characterization of a Novel Human CC Chemokine Receptor (CCR5) for RANTES, MIP-1β, and MIP-1α* , 1996, The Journal of Biological Chemistry.

[8]  C. Combadière,et al.  Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MIP‐1α, MIP‐1β, and RANTES , 1996, Journal of leukocyte biology.

[9]  C. Broder,et al.  CC CKR5: A RANTES, MIP-1α, MIP-1ॆ Receptor as a Fusion Cofactor for Macrophage-Tropic HIV-1 , 1996, Science.

[10]  Marc Parmentier,et al.  A Dual-Tropic Primary HIV-1 Isolate That Uses Fusin and the β-Chemokine Receptors CKR-5, CKR-3, and CKR-2b as Fusion Cofactors , 1996, Cell.

[11]  Ying Sun,et al.  The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Primary HIV-1 Isolates , 1996, Cell.

[12]  A. Luster,et al.  Identification of a mouse eosinophil receptor for the CC chemokine eotaxin. , 1996, Biochemical and biophysical research communications.

[13]  Virginia Litwin,et al.  HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5 , 1996, Nature.

[14]  Stephen C. Peiper,et al.  Identification of a major co-receptor for primary isolates of HIV-1 , 1996, Nature.

[15]  P. Murphy.  Chemokine receptors: structure, function and role in microbial pathogenesis. , 1996, Cytokine & growth factor reviews.

[16]  C. Mackay,et al.  Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils , 1996, The Journal of experimental medicine.

[17]  Paul E. Kennedy,et al.  HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein-Coupled Receptor , 1996, Science.

[18]  C. Combadière,et al.  Molecular Cloning of Human Eotaxin, an Eosinophil-selective CC Chemokine, and Identification of a Specific Eosinophil Eotaxin Receptor, CC Chemokine Receptor 3 (*) , 1996, The Journal of Biological Chemistry.

[19]  G Vassart,et al.  Molecular cloning and functional expression of a new human CC-chemokine receptor gene. , 1996, Biochemistry.

[20]  W Newman,et al.  Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. , 1996, The Journal of clinical investigation.

[21]  C. Power,et al.  Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4. , 1996, Biochemical and biophysical research communications.

[22]  J. Van Damme,et al.  Monocyte Chemoattractant Protein-3 Is a Functional Ligand for CC Chemokine Receptors 1 and 2B (*) , 1995, The Journal of Biological Chemistry.

[23]  A. Dvorak,et al.  Infection, apoptosis, and killing of mature human eosinophils by human immunodeficiency virus-1. , 1995, American journal of respiratory cell and molecular biology.

[24]  T. Williams,et al.  Cooperation between interleukin-5 and the chemokine eotaxin to induce eosinophil accumulation in vivo , 1995, The Journal of experimental medicine.

[25]  C. Broder,et al.  Fusogenic selectivity of the envelope glycoprotein is a major determinant of human immunodeficiency virus type 1 tropism for CD4+ T-cell lines vs. primary macrophages. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[26]  C. Power,et al.  Molecular Cloning and Functional Expression of a Novel CC Chemokine Receptor cDNA from a Human Basophilic Cell Line (*) , 1995, The Journal of Biological Chemistry.

[27]  C. Combadière,et al.  Cloning, chromosomal localization, and RNA expression of a human beta chemokine receptor-like gene. , 1995, DNA and cell biology.

[28]  C. Combadière,et al.  Cloning and Functional Expression of a Human Eosinophil CC Chemokine Receptor (*) , 1995, The Journal of Biological Chemistry.

[29]  Fei Li,et al.  Hyperglycosylation of eosinophil ribonucleases in a promyelocytic leukemia cell line and in differentiated peripheral blood progenitor cells , 1995, Journal of leukocyte biology.

[30]  C. Broder,et al.  Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation , 1994, Journal of virology.

[31]  D. Nicholson,et al.  Induction, characterization, and functional coupling of the high affinity chemokine receptor for RANTES and macrophage inflammatory protein-1 alpha upon differentiation of an eosinophilic HL-60 cell line. , 1994, Journal of immunology.

[32]  S. Coughlin,et al.  Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[33]  U. Francke,et al.  Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor , 1993, The Journal of experimental medicine.

[34]  C. Broder,et al.  The block to HIV-1 envelope glycoprotein-mediated membrane fusion in animal cells expressing human CD4 can be overcome by a human cell component(s). , 1993, Virology.

[35]  T. Schall,et al.  Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor , 1993, Cell.

[36]  T. Schlake,et al.  Scaffold-attached regions (SAR elements) mediate transcriptional effects due to butyrate. , 1992, Biochemistry.

[37]  G. Griffin,et al.  Human immunodeficiency virus infection of eosinophils in human bone marrow cultures , 1991, The Journal of experimental medicine.

[38]  P. Weller,et al.  The immunobiology of eosinophils. , 1991, The New England journal of medicine.

[39]  J. Tavernier,et al.  Characterization of interleukin 5 receptors on eosinophilic sublines from human promyelocytic leukemia (HL-60) cells , 1990, The Journal of experimental medicine.

[40]  S. Fischkoff.  Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions. , 1988, Leukemia research.

[41]  B. Moss,et al.  Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. , 1986, Proceedings of the National Academy of Sciences of the United States of America.