Antibacterial activity of chitosan and the interpolyelectrolyte complexes of poly(acrylic acid)-chitosan

A atividade antimicrobiana de quitosana e complexos interpolieletroliticos hidrossoluveis de poli(acido acrilico)-quitosana foi estudada. Quitosanas de dois diferentes pesos moleculares foram testados em diferentes concentracoes, 0,5 a 5 g • L-1, como agentes antimicrobianos nas P. aeruginosa e P. oleovorans. Em ambos os casos, obteu-se a melhor inibicao microbiana com a concentracao de 5 g • L-1, no entanto os complexos interpolieletroliticos de poli (acido acrilico)-quitosana com composicao φ = 2 apresentaram maior atividade antibacteriana do que os dois quitosans na concentracao de 0,5 g • L-1. O complexo NPEC2 foi mais eficaz do que as quitosanas, sendo que o resultado pode ser atribuido ao numero de moles dos grupos aminos da quitosana e aos grupos carboxilicos dos complexos de poli(acido acrilico).

[1]  Y. Shin,et al.  Molecular weight effect on antimicrobial activity of chitosan treated cotton fabrics , 2001 .

[2]  V. Kabanov,et al.  Soluble interpolymeric complexes as a new class of synthetic polyelectrolytes , 1984 .

[3]  Jen-Ming Yang,et al.  Wettability and antibacterial assessment of chitosan containing radiation‐induced graft nonwoven fabric of polypropylene‐g‐acrylic acid , 2003 .

[4]  N. Pace.  A molecular view of microbial diversity and the biosphere. , 1997, Science.

[5]  Yingchien Chung,et al.  Effect of abiotic factors on the antibacterial activity of chitosan against waterborne pathogens. , 2003, Bioresource technology.

[6]  F. Ausubel,et al.  Caenorhabditis elegans: a model genetic host to study Pseudomonas aeruginosa pathogenesis. , 2000, Current opinion in microbiology.

[7]  Mattheus F. A. Goosen,et al.  pH‐sensitivity of hydrogels based on complex forming chitosan: Polyether interpenetrating polymer network , 1993 .

[8]  David A. D'Argenio,et al.  Drosophilaas a Model Host forPseudomonas aeruginosaInfection , 2001, Journal of bacteriology.

[9]  Norio Nagao,et al.  Effects of Chitosan, Pectic Acid, Lysozyme, and Chitinase on the Growth of Several Phytopathogens , 1989 .

[10]  G. Peterson,et al.  A simplification of the protein assay method of Lowry et al. which is more generally applicable. , 1977, Analytical biochemistry.

[11]  S. Hudson,et al.  Review of Chitosan and Its Derivatives as Antimicrobial Agents and Their Uses as Textile Chemicals , 2003 .

[12]  M. Struszczyk,et al.  Characterization of chitosan , 1998 .

[13]  A. Benavides-Mendoza,et al.  Use of the Interpolyelectrolyte Complexes of Poly(acrylic acid)-Chitosan as Inductors of Tolerance Against Pathogenic Fungi in Tomato (Lycopersicon esculentum Mill. var. Floradade) , 2003 .

[14]  K. Yao,et al.  Antibacterial action of chitosan and carboxymethylated chitosan , 2001 .

[15]  J. Desbrières,et al.  An infrared investigation in relation with chitin and chitosan characterization , 2001 .

[16]  M. Smogyi,et al.  Notes on sugar determination. , 1952, The Journal of biological chemistry.

[17]  S. Edberg,et al.  Pseudomonas aeruginosa: assessment of risk from drinking water. , 1997, Critical reviews in microbiology.

[18]  H. Mark,et al.  ViscosityMolecular Weight Relationship for Cellulose Acetate. , 1947 .

[19]  M. Rinaudo,et al.  Characterization of chitosan. Influence of ionic strength and degree of acetylation on chain expansion. , 1993, International journal of biological macromolecules.

[20]  Norton Nelson,et al.  A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE , 1944 .

[21]  F. Ausubel,et al.  Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors. , 1997, Proceedings of the National Academy of Sciences of the United States of America.