The design of rapid MicroRNA detection system

In order to detect miRNA quickly, we designed a new portable device for the rapid detection of miRNA, using Opto-electronic detection technology, marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity, the recognition system of characteristic fluorescence analysis was established. By changing the excitation light intensity, miRNA reagent concentration and other parameters, we arrive at the conclusion that there is the linear relationship (R2=0.9947) between miRNA concentration and fluorescence intensity when the miRNA concentration range the instrument can measure is in the range of 0.01-0.1mol and the lowest values measured by the instrument in theory is 7 copies.

[1]  K. Livak,et al.  Real-time quantification of microRNAs by stem–loop RT–PCR , 2005, Nucleic acids research.

[2]  Ana Kozomara,et al.  miRBase: integrating microRNA annotation and deep-sequencing data , 2010, Nucleic Acids Res..

[3]  Bin Zhao,et al.  Discriminative identification of miRNA let-7 family members with high specificity and sensitivity using rolling circle amplification. , 2015, Acta biochimica et biophysica Sinica.

[4]  Yongxin Ma,et al.  A microarray for microRNA profiling in mouse testis tissues. , 2007, Reproduction.

[5]  Yariv Yogev,et al.  Serum MicroRNAs Are Promising Novel Biomarkers , 2008, PloS one.

[6]  C. Burge,et al.  Most mammalian mRNAs are conserved targets of microRNAs. , 2008, Genome research.

[7]  S. Deo,et al.  Trends in microRNA detection , 2009, Analytical and bioanalytical chemistry.

[8]  C. M. Jefferson,et al.  Design and performance of a refractive optical system that converts a Gaussian to a flattop beam. , 2000, Applied optics.

[9]  Erin K Hanson,et al.  Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. , 2009, Analytical biochemistry.

[10]  K. Kurabayashi,et al.  PDMS-based opto-fluidic micro flow cytometer with two-color, multi-angle fluorescence detection capability using PIN photodiodes , 2004 .

[11]  Hui-Chung Wu,et al.  DNA sequencing using rolling circle amplification and precision glass syringes in a high-throughput liquid handling system. , 2003, BioTechniques.

[12]  V. Demidov,et al.  Rolling-circle amplification in DNA diagnostics: the power of simplicity , 2002, Expert review of molecular diagnostics.