The omnipotent suppressor SUP45 affects nucleic acid metabolism and mitochondrial structure

Yeast (Saccharomyces cerevisiae) strains sensitive to a variety drugs were used to select for novobiocin‐resistant mutants that were simultaneously temperature‐sensitive. The mutants remained as sensitive as the parent strains to a wide range of drugs other than novobiocin, and did not exhibit any suppression of suppressible auxotrophic markers. At the non‐permissive temperature, the mutant cells arrested mainly as unbudded cells, and were instantly defective in DNA and RNA synthesis, but not protein synthesis. The cloned wild‐type gene was identified as SUP45, which has been previously implicated in the translation process. Our results suggest that SUP45 may have a function in addition to, or different from, the one that has been assigned to it previously.

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