Laser‐Induced Fluorescence for Esophageal Cancer and Dysplasia Diagnosis a

A method using laser-induced fluorescence (LIF) for in vivo cancer diagnosis of the esophagus is described. Autofluorescence of normal and malignant tissues was measured directly using a fiberoptic probe inserted through an endoscope. The measurements were performed in vivo during routine endoscopy. Measurement of the fluorescence signal from the tissue was performed using laser excitation at 410 nm. The methodology was applied to differentiate normal and malignant tumors of the esophagus. The results of this LIF approach were compared with histopathology results of the biopsy samples and indicated excellent agreement in the classification of normal and malignant tumors for the samples investigated. The LIF procedure could lead to the development of a rapid and cost-effective technique for cancer diagnosis.

[1]  Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples. , 1991, Clinical chemistry.

[2]  D. Choy,et al.  Fluorescence spectra from cancerous and normal human breast and lung tissues , 1987, Annual Meeting Optical Society of America.

[3]  R M Cothren,et al.  Gastrointestinal tissue diagnosis by laser-induced fluorescence spectroscopy at endoscopy. , 1990, Gastrointestinal endoscopy.

[4]  R R Alfano,et al.  Pulsed and cw laser fluorescence spectra from cancerous, normal, and chemically treated normal human breast and lung tissues. , 1989, Applied optics.

[5]  L. Deckelbaum,et al.  Laser-induced fluorescence spectroscopy of human colonic mucosa. Detection of adenomatous transformation. , 1990, Gastroenterology.

[6]  B F Overholt,et al.  Quantification of phthalocyanine concentration in rat tissue using laser–induced fluorescence spectroscopy , 1993, Lasers in surgery and medicine.

[7]  Tuan Vo-Dinh,et al.  Laser-Induced Differential Fluorescence for Cancer Diagnosis without Biopsy , 1997 .

[8]  D M Parkin,et al.  Estimates of the worldwide frequency of sixteen major cancers in 1980 , 1988, International journal of cancer.

[9]  T J Flotte,et al.  Ultraviolet laser‐induced fluorescence of colonic tissue: Basic biology and diagnostic potential , 1992, Lasers in surgery and medicine.

[10]  Rick E. Sneed,et al.  In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices , 1995, Lasers in surgery and medicine.