A signal-on electrochemiluminescence biosensor for detecting Con A using phenoxy dextran-graphite-like carbon nitride as signal probe.

A novel signal-on electrochemiluminescence (ECL) biosensor for detecting concanavalin A (Con A) was fabricated with phenoxy dextran-graphite-like carbon nitride (DexP-g-C3N4) as signal probe. In this construction strategy, the nanocomposites of three-dimensional graphene and gold nanoparticles (3D-GR-AuNPs) were used as matrix for high loading of glucose oxidase (GOx), which served as recognition element for bounding Con A. Con A further interacted with DexP-g-C3N4 through a specific carbohydrate-Con A interaction to achieve a sandwiched scheme. With the increase of Con A incubated onto the electrode, the ECL signal resulted from DexP-g-C3N4 would enhance, thus achieving a signal-on ECL biosensor for Con A detection. Due to the integration of the virtues of 3D-GR-AuNPs and the excellent ECL performance of DexP-g-C3N4, the prepared biosensor exhibits a wide linear response range from 0.05 ng/mL to 100 ng/mL and a low detection limit of 17 pg/mL (S/N=3).

[1]  Ruo Yuan,et al.  A signal-on electrochemical aptasensor for ultrasensitive detection of endotoxin using three-way DNA junction-aided enzymatic recycling and graphene nanohybrid for amplification. , 2014, Nanoscale.

[2]  Ashok Mulchandani,et al.  Single-walled carbon nanotube-based chemiresistive affinity biosensors for small molecules: ultrasensitive glucose detection. , 2010, Journal of the American Chemical Society.

[3]  R. Schlögl,et al.  Graphitic carbon nitride materials: variation of structure and morphology and their use as metal-free catalysts , 2008 .

[4]  Hong Dai,et al.  An electrochemiluminescent sensor for methamphetamine hydrochloride based on multiwall carbon nanotube/ionic liquid composite electrode. , 2009, Biosensors & bioelectronics.

[5]  Yueping Fang,et al.  Synthesis of porous Fe3O4/g-C3N4 nanospheres as highly efficient and recyclable photocatalysts , 2013 .

[6]  Juan Tang,et al.  Displacement-type quartz crystal microbalance immunosensing platform for ultrasensitive monitoring of small molecular toxins. , 2013, Analytical chemistry.

[7]  Chih-Ching Huang,et al.  Synthesis of fluorescent carbohydrate-protected Au nanodots for detection of Concanavalin A and Escherichia coli. , 2009, Analytical chemistry.

[8]  D. Xing,et al.  Ultrasensitive aptamer-based bio bar code immunomagnetic separation and electrochemiluminescence method for the detection of protein. , 2012, Analytica chimica acta.

[9]  D. Xing,et al.  A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein. , 2010, Biosensors & bioelectronics.

[10]  D. Xiao,et al.  Anodic electrogenerated chemiluminescence behavior of graphite-like carbon nitride and its sensing for rutin. , 2013, Analytical chemistry.

[11]  Xuping Sun,et al.  Au-nanoparticle-loaded graphitic carbon nitride nanosheets: green photocatalytic synthesis and application toward the degradation of organic pollutants. , 2013, ACS applied materials & interfaces.

[12]  J. Pongrácz,et al.  Con A activates an Akt/PKB dependent survival mechanism to modulate TCR induced cell death in double positive thymocytes. , 2003, Molecular immunology.

[13]  Q. Ma,et al.  A novel signal-off electrochemiluminescence biosensor for the determination of glucose based on double nanoparticles. , 2015, Biosensors & bioelectronics.

[14]  Guonan Chen,et al.  Gold nanoparticle-graphite-like C3N4 nanosheet nanohybrids used for electrochemiluminescent immunosensor. , 2014, Analytical chemistry.

[15]  Jinghong Li,et al.  Dynamic evaluation of cell surface N-glycan expression via an electrogenerated chemiluminescence biosensor based on concanavalin A-integrating gold-nanoparticle-modified Ru(bpy)3(2+)-doped silica nanoprobe. , 2013, Analytical chemistry.

[16]  Hua Zhang,et al.  Graphene-based composites. , 2012, Chemical Society reviews.

[17]  Martin M. F. Choi,et al.  Electrogenerated chemiluminescence behavior of graphite-like carbon nitride and its application in selective sensing Cu2+. , 2012, Analytical chemistry.

[18]  Bicai Pan,et al.  Enhanced photoresponsive ultrathin graphitic-phase C3N4 nanosheets for bioimaging. , 2013, Journal of the American Chemical Society.

[19]  Yaofang Hu,et al.  Label-free electrochemical impedance spectroscopy biosensor for direct detection of cancer cells based on the interaction between carbohydrate and lectin. , 2013, Biosensors & bioelectronics.

[20]  G. Shi,et al.  Self-assembled graphene hydrogel via a one-step hydrothermal process. , 2010, ACS nano.

[21]  Liangti Qu,et al.  A versatile, ultralight, nitrogen-doped graphene framework. , 2012, Angewandte Chemie.

[22]  Q. Wei,et al.  Quenched electrochemiluminescence of Ag nanoparticles functionalized g-C₃N₄ by ferrocene for highly sensitive immunosensing. , 2015, Analytica chimica acta.

[23]  Abdullah M. Asiri,et al.  Ultrathin graphitic carbon nitride nanosheets: a low-cost, green, and highly efficient electrocatalyst toward the reduction of hydrogen peroxide and its glucose biosensing application. , 2013, Nanoscale.

[24]  P. Chan,et al.  Trityl-derivatized carbohydrates immobilized on a polystyrene microplate. , 2008, Carbohydrate research.

[25]  Ruo Yuan,et al.  Electrochemiluminescence of luminol enhanced by the synergetic catalysis of hemin and silver nanoparticles for sensitive protein detection. , 2014, Biosensors & bioelectronics.

[26]  Y. Chai,et al.  In situ generation of self-enhanced luminophore by β-lactamase catalysis for highly sensitive electrochemiluminescent aptasensor. , 2014, Analytical chemistry.

[27]  Shihong Chen,et al.  Electrochemiluminescence biosensor for cholesterol detection based on AuNPs/L-cys–C60 nanocomposites , 2014 .

[28]  Jun-ichi Anzai,et al.  Preparation and optimization of bienzyme multilayer films using lectin and glyco-enzymes for biosensor applications , 2001 .

[29]  Qian Liu,et al.  Ultrathin graphitic carbon nitride nanosheet: a highly efficient fluorosensor for rapid, ultrasensitive detection of Cu(2+). , 2013, Analytical chemistry.

[30]  R. Yuan,et al.  An electrogenerated chemiluminescence sensor based on gold nanoparticles@C60 hybrid for the determination of phenolic compounds. , 2014, Biosensors & bioelectronics.

[31]  Jianding Qiu,et al.  "On-off" switchable electrochemical affinity nanobiosensor based on graphene oxide for ultrasensitive glucose sensing. , 2013, Biosensors & bioelectronics.

[32]  Iraida Loinaz,et al.  Nanostructured disposable impedimetric sensors as tools for specific biomolecular interactions: sensitive recognition of concanavalin A. , 2011, Analytical chemistry.

[33]  Jian-Ding Qiu,et al.  Graphene oxide and dextran capped gold nanoparticles based surface plasmon resonance sensor for sensitive detection of concanavalin A. , 2013, Biosensors & bioelectronics.

[34]  Xiangqun Zeng,et al.  Complex thiolated mannose/quinone film modified on EQCM/Au electrode for recognizing specific carbohydrate-proteins. , 2014, Biosensors & bioelectronics.

[35]  Jinghua Yu,et al.  A disposable electrochemiluminescence device for ultrasensitive monitoring of K562 leukemia cells based on aptamers and ZnO@carbon quantum dots. , 2013, Biosensors & bioelectronics.

[36]  Lun Wang,et al.  Cathodic electrochemiluminescence behavior of norfloxacin/peroxydisulfate system in purely aqueous solution , 2008 .

[37]  Long Jiang,et al.  Highly sensitive gold nanoparticles biosensor chips modified with a self-assembled bilayer for detection of Con A. , 2007, Biosensors & bioelectronics.

[38]  Guonan Chen,et al.  Preparation of graphite-like carbon nitride nanoflake film with strong fluorescent and electrochemiluminescent activity. , 2013, Nanoscale.

[39]  Changcun Han,et al.  Synthesis of MWNTs/g-C3N4 composite photocatalysts with efficient visible light photocatalytic hydrogen evolution activity , 2012 .

[40]  Chengming Wang,et al.  Enhancement of electrogenerated chemiluminescence of luminol by ascorbic acid at gold nanoparticle/graphene modified glassy carbon electrode. , 2015, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[41]  G. Wallace,et al.  Mechanically Strong, Electrically Conductive, and Biocompatible Graphene Paper , 2008 .

[42]  R. Yuan,et al.  An electrogenerated chemiluminescence sensor prepared with a graphene/multiwall carbon nanotube/gold nanocluster hybrid for the determination of phenolic compounds. , 2013, The Analyst.

[43]  SUPARNA DUTTASINHA,et al.  Graphene: Status and Prospects , 2009, Science.

[44]  R. Yuan,et al.  Enhanced electrochemiluminescence sensor for detecting dopamine based on gold nanoflower@graphitic carbon nitride polymer nanosheet-polyaniline hybrids. , 2014, The Analyst.

[45]  R. Yuan,et al.  Multi-wall carbon nanotube-polyaniline biosensor based on lectin-carbohydrate affinity for ultrasensitive detection of Con A. , 2012, Biosensors & bioelectronics.