论文信息 - Single-Molecule Arrays for Ultrasensitive Detection of Host Immune Response to Dengue Virus Infection

Single-Molecule Arrays for Ultrasensitive Detection of Host Immune Response to Dengue Virus Infection

ABSTRACT Dengue virus serotype 2-positive plasma was applied to two indirect single-molecule arrays (Simoas) for the detection of anti-dengue virus IgG and IgM. The Simoas were 1,000 and 10,000 times more sensitive than enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM, respectively. Using Simoas, serology may be used for the detection of dengue virus infection in the acute phase.

David R. Walt | Shonda T. Gaylord | Sama Abdul-Aziz

[1] David H Wilson,et al. Simultaneous detection of single molecules and singulated ensembles of molecules enables immunoassays with broad dynamic range. , 2011, Analytical chemistry.

[2] David M. Rissin,et al. Single-Molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations , 2010, Nature Biotechnology.

[3] G. Malavige,et al. Dengue viral infections , 2004, Postgraduate Medical Journal.

[4] Kun Wen,et al. Kinetics of non-structural protein 1, IgM and IgG antibodies in dengue type 1 primary infection , 2011, Virology Journal.

[5] S. Bhatia,et al. Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers , 2014, Journal of the American Chemical Society.

[6] David R Walt,et al. Direct detection of bacterial genomic DNA at sub-femtomolar concentrations using single molecule arrays. , 2013, Analytical chemistry.

[7] F. Teles,et al. Biosensors and rapid diagnostic tests on the frontier between analytical and clinical chemistry for biomolecular diagnosis of dengue disease: A review , 2010, Analytica Chimica Acta.

[8] David H Wilson,et al. Simple diffusion-constrained immunoassay for p24 protein with the sensitivity of nucleic acid amplification for detecting acute HIV infection. , 2013, Journal of virological methods.

[9] Linan Song,et al. Multiplexed single molecule immunoassays. , 2013, Lab on a chip.