Comparison of microscopic methods for evaluating platelet adhesion to biomaterial surfaces.

Microscopic methods usable for sample surface imaging and subsequent qualitative and quantitative evaluation of platelet adhesion to the surface of the biomaterial studied were compared. It was shown, making use of the samples of medical steel (AISI 316L), that such tools as surface imaging with scanning electron microscopy (SEM), glutaraldehyde induced fluorescence technique (GIFT) and metallurgical microscopy (MM) are equivalent in evaluating surface platelet adhesion. The importance of biological variability of blood samples for a proper result assessment and the necessity of using internal standards were also considered.

[1]  S. Pan,et al.  Structure and blood compatibility of tetrahedral amorphous hydrogenated carbon formed by a magnetic-field-filter plasma stream☆ , 2007 .

[2]  A. Hotta,et al.  Effects of surface roughness on anti-thrombogenicity of diamond-like carbon films , 2007 .

[3]  N. Huang,et al.  Activation of platelets adhered on amorphous hydrogenated carbon (a-C:H) films synthesized by plasma immersion ion implantation-deposition (PIII-D). , 2003, Biomaterials.

[4]  Chirathodi Vayalappil Muraleedharan,et al.  Quantitation of platelet adhesion to Ti and DLC-coated Ti in vitro using (125)I-labeled platelets. , 2002, Biomolecular engineering.

[5]  G. Lip,et al.  Platelet activation: assessment and quantification. , 2001, European heart journal.

[6]  H. Thelen,et al.  Glutardialdehyde induced fluorescence technique (GIFT): a new method for the imaging of platelet adhesion on biomaterials. , 2000, Journal of biomedical materials research.

[7]  I. R. McColl,et al.  Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications. , 2000, Journal of biomedical materials research.

[8]  S. Goodman,et al.  Sheep, pig, and human platelet-material interactions with model cardiovascular biomaterials. , 1999, Journal of biomedical materials research.

[9]  H. Nygren.  Initial reactions of whole blood with hydrophilic and hydrophobic titanium surfaces , 1996 .

[10]  British Standard,et al.  Biological evaluation of medical devices , 2006 .

[11]  K. R. Resmi,et al.  Procedure for quantification of platelet adhesion to biomaterials by radioscintigraphy. , 2004, Thrombosis Research.

[12]  P. Tengvall,et al.  Platelet binding and protein adsorption to titanium and gold after short time exposure to heparinized plasma and whole blood. , 1996, Biomaterials.