Biohydrogen Production from Wastewater

Abstract Biohydrogen (H2) production from renewable wastewater signifies a sustainable alternative to a fossil fuel-based economy. H2 is deemed to be the futuristic energy carrier with a high-energy yield. This chapter specifically addresses H2 production through wastewater remediation. A comprehensive emphasis is made on the fermentative routes, namely, light-driven and light-independent processes, encompassing wastewater and mixed culture application as a focal point. Important process parameters that have critical roles on process efficiency are elaborated. Some of the persistent limitations encountered during the process operation are delineated. Stress is made on the pertinent discussion on biocatalyst pretreatment methods in the context of mixed culture wastewater usage. Reported strategies to overcome the limitations are discussed to make the process economically viable, especially for large-scale applications.

[1]  N. Jakubovics,et al.  Out of the iron age: new insights into the critical role of manganese homeostasis in bacteria. , 2001, Microbiology.

[2]  Hong Liu,et al.  Effect of pH on hydrogen production from glucose by a mixed culture. , 2002, Bioresource technology.

[3]  Alfons J. M. Stams,et al.  Electron transfer in syntrophic communities of anaerobic bacteria and archaea , 2009, Nature Reviews Microbiology.

[4]  Jeongsik Kim,et al.  Effects of various pretreatments for enhanced anaerobic digestion with waste activated sludge. , 2003, Journal of bioscience and bioengineering.

[5]  Richard Sparling,et al.  Hydrogen production from inhibited anaerobic composters , 1997 .

[6]  S. Sung,et al.  Biological hydrogen production in an anaerobic sequencing batch reactor: pH and cyclic duration effects , 2009 .

[7]  James I. Chang,et al.  Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge , 2003 .

[8]  I. Eroglu,et al.  Effect of iron and molybdenum addition on photofermentative hydrogen production from olive mill wastewater , 2011 .

[9]  Ebru Özgür,et al.  Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions , 2012 .

[10]  J. Matsumoto,et al.  Fundamental Studies on Hydrogen Production in the Acid-Forming Phase and Its Bacteria in Anaerobic Treatment Processes – The Effects of Solids Retention Time , 1993 .

[11]  K. Seifert,et al.  Hydrogen generation in photobiological process from dairy wastewater , 2010 .

[12]  Bo Wang,et al.  Effect of ammonia concentration on fermentative hydrogen production by mixed cultures. , 2009, Bioresource technology.

[13]  Sang-Eun Oh,et al.  Biohydrogen gas production from food processing and domestic wastewaters , 2005 .

[14]  Richard Sparling,et al.  Hydrogen generation via anaerobic fermentation of paper mill wastes. , 2005, Bioresource technology.

[15]  Jo-Shu Chang,et al.  Batch and continuous biohydrogen production from starch hydrolysate by Clostridium species , 2008 .

[16]  S. Mohan,et al.  Acidic and alkaline shock pretreatment to enrich acidogenic biohydrogen producing mixed culture: long term synergetic evaluation of microbial inventory, dehydrogenase activity and bio-electro kinetics , 2012 .

[17]  Chae-Young Lee,et al.  Enhancement of hydrogen production by recycling of methanogenic effluent in two-phase fermentation of food waste , 2012 .

[18]  Debabrata Das,et al.  Microbial hydrogen production from sewage sludge bioaugmented with a constructed microbial consortium , 2010 .

[19]  G Onkal Engin,et al.  Treatment efficiency and VFA composition of a thermophilic anaerobic contact reactor treating food industry wastewater. , 2010, Journal of hazardous materials.

[20]  S. Venkata Mohan,et al.  Waste to Renewable Energy: A Sustainable and Green Approach Towards Production of Biohydrogen by Acidogenic Fermentation , 2010 .

[21]  Gustavo Davila-Vazquez,et al.  Fermentative hydrogen production in batch experiments using lactose, cheese whey and glucose: Influence of initial substrate concentration and pH , 2008 .

[22]  S. V. Mohan.  Fermentative hydrogen production with simultaneous wastewater treatment : influence of pretreatment and system operating conditions , 2008 .

[23]  Yan-lin Zhang,et al.  Effects of various pretreatment methods of anaerobic mixed microflora on biohydrogen production and the fermentation pathway of glucose , 2011 .

[24]  A. Reungsang,et al.  Biohydrogen production from waste glycerol and sludge by anaerobic mixed cultures , 2012 .

[25]  Inhibition on acidogenesis of dairy wastewater by zinc and copper. , 2001, Environmental technology.

[26]  P. N. Sarma,et al.  Harnessing of biohydrogen by acidogenic fermentation of Citrus limetta peelings: Effect of extraction procedure and pretreatment of biocatalyst , 2009 .

[27]  Lars Rohlin,et al.  Physiology, Ecology, Phylogeny, and Genomics of Microorganisms Capable of Syntrophic Metabolism , 2008, Annals of the New York Academy of Sciences.

[28]  T. Keskin,et al.  Hydrogen production from sugar industry wastes using single-stage photofermentation. , 2012, Bioresource technology.

[29]  S. Venkata Mohan,et al.  Regulatory function of divalent cations in controlling the acidogenic biohydrogen production process , 2012 .

[30]  P. Hallenbeck,et al.  The effect of nutrient limitation on hydrogen production by batch cultures of Escherichia coli , 2006 .

[31]  Peter Seto,et al.  Buffer requirements for enhanced hydrogen production in acidogenic digestion of food wastes. , 2009, Bioresource technology.

[32]  F. Liu,et al.  Evaluation of different pretreatment methods for preparing hydrogen-producing seed inocula from waste activated sludge , 2011 .

[33]  Heguang Zhu,et al.  Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge , 2006 .

[34]  E. Crabbe,et al.  Influence of initial pH on hydrogen production from cheese whey. , 2005, Journal of biotechnology.

[35]  F. Kargı,et al.  Photo-fermentative hydrogen gas production from dark fermentation effluent of acid hydrolyzed wheat starch with periodic feeding , 2011 .

[36]  L. T. Angenent,et al.  Production of bioenergy and biochemicals from industrial and agricultural wastewater. , 2004, Trends in biotechnology.

[37]  Alissara Reungsang,et al.  Co-digestion of food waste and sludge for hydrogen production by anaerobic mixed cultures: Statistic , 2011 .

[38]  Qiang Liao,et al.  Characteristics of hydrogen production and substrate consumption of Rhodopseudomonas palustris CQK 01 in an immobilized-cell photobioreactor. , 2010, Bioresource technology.

[39]  Chiu-Yue Lin,et al.  Heavy metal effects on fermentative hydrogen production using natural mixed microflora , 2008 .

[40]  Daling Zhu,et al.  Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods , 2009 .

[41]  Mi‐Sun Kim,et al.  Effect of carbon and nitrogen sources on photo-fermentative H2 production associated with nitrogenase, uptake hydrogenase activity, and PHB accumulation in Rhodobacter sphaeroides KD131. , 2012, Bioresource technology.

[42]  John R. Benemann,et al.  Biological hydrogen production , 1995 .

[43]  L. Chen,et al.  Induction of a Futile Embden-Meyerhof-Parnas Pathway in Deinococcus radiodurans by Mn: Possible Role of the Pentose Phosphate Pathway in Cell Survival , 2000, Applied and Environmental Microbiology.

[44]  N. Ren,et al.  Enhanced bio-hydrogen production by the combination of dark- and photo-fermentation in batch culture. , 2010, Bioresource technology.

[45]  Hong Liu,et al.  Electrochemically assisted microbial production of hydrogen from acetate. , 2005, Environmental science & technology.

[46]  M. Levitt,et al.  Factors affecting hydrogen production and consumption by human fecal flora. The critical roles of hydrogen tension and methanogenesis. , 1992, The Journal of clinical investigation.

[47]  Haifeng Lu,et al.  Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation: different metabolic pathways. , 2011, Bioresource technology.

[48]  Willy Verstraete,et al.  Methanogenesis in membraneless microbial electrolysis cells , 2009, Applied Microbiology and Biotechnology.

[49]  N. Ren,et al.  Assessing optimal fermentation type for bio-hydrogen production in continuous-flow acidogenic reactors. , 2007, Bioresource technology.

[50]  Sean Tyrrel,et al.  The effect of organic loading rate on foam initiation during mesophilic anaerobic digestion of municipal wastewater sludge. , 2011, Bioresource technology.

[51]  Peter Seto,et al.  Co-production of hydrogen and methane from potato waste using a two-stage anaerobic digestion process. , 2008, Bioresource technology.

[52]  T. R. Sreekrishnan,et al.  Enhancement of biogas production from solid substrates using different techniques--a review. , 2004, Bioresource technology.

[53]  F. Kargı,et al.  Hydrogen gas production from cheese whey powder (CWP) solution by thermophilic dark fermentation , 2012 .

[54]  P. N. Sarma,et al.  Metabolic shift and electron discharge pattern of anaerobic consortia as a function of pretreatment method applied during fermentative hydrogen production , 2010 .

[55]  M. Maguire,et al.  Magnesium transport in prokaryotes , 1999, JBIC Journal of Biological Inorganic Chemistry.

[56]  P. N. Sarma,et al.  Insight into the dehydrogenase catalyzed redox reactions and electron discharge pattern during fermentative hydrogen production. , 2010, Bioresource technology.

[57]  F. Kargı,et al.  Bio-hydrogen production from cheese whey powder (CWP) solution: Comparison of thermophilic and mesophilic dark fermentations , 2012 .

[58]  Mi-Sun Kim,et al.  Fermentative hydrogen production from tofu-processing waste and anaerobic digester sludge using microbial consortium. , 2010, Bioresource technology.

[59]  D. Kahn,et al.  Genetic regulation of biological nitrogen fixation , 2004, Nature Reviews Microbiology.

[60]  I. Eroglu,et al.  Effects of ammonium ion, acetate and aerobic conditions on hydrogen production and expression levels of nitrogenase genes in Rhodobacter sphaeroides O.U.001 , 2009 .

[61]  Bruce E. Logan,et al.  A monetary comparison of energy recovered from microbial fuel cells and microbial electrolysis cells fed winery or domestic wastewaters , 2010 .

[62]  S. Venkata Mohan,et al.  Dehydrogenase activity in association with poised potential during biohydrogen production in single chamber microbial electrolysis cell. , 2011 .

[63]  Jianlong Wang,et al.  Effect of Fe2+ concentration on fermentative hydrogen production by mixed cultures , 2008 .

[64]  Duu-Jong Lee,et al.  Biological hydrogen production from phenol-containing wastewater using Clostridium butyricum , 2010 .

[65]  D. Karakashev,et al.  Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures , 2012, Biotechnology for Biofuels.

[66]  K Bala Amutha,et al.  Biological hydrogen production by the algal biomass Chlorella vulgaris MSU 01 strain isolated from pond sediment. , 2011, Bioresource technology.

[67]  S. O-thong,et al.  Effect of initial pH, nutrients and temperature on hydrogen production from palm oil mill effluent using thermotolerant consortia and corresponding microbial communities , 2012 .

[68]  A. Mohammad,et al.  A holistic approach to managing palm oil mill effluent (POME): biotechnological advances in the sustainable reuse of POME. , 2009, Biotechnology advances.

[69]  Marc A. Rosen,et al.  Entropy production and exergy destruction: Part II—illustrative technologies , 2003 .

[70]  R. Lakshmidevi,et al.  Enzymatic saccharification and fermentation of paper and pulp industry effluent for biohydrogen production , 2010 .

[71]  Germán Buitrón,et al.  Biohydrogen production from Tequila vinasses in an anaerobic sequencing batch reactor: effect of initial substrate concentration, temperature and hydraulic retention time. , 2010, Bioresource technology.

[72]  I. Eroglu,et al.  Biological hydrogen production from olive mill wastewater with two-stage processes , 2006 .

[73]  Xiaobing Wu,et al.  Enhanced H2 gas production from bagasse using adhE inactivated Klebsiella oxytoca HP1 by sequential dark-photo fermentations. , 2010, Bioresource technology.

[74]  Jo‐Shu Chang,et al.  Outlook of biohydrogen production from lignocellulosic feedstock using dark fermentation - A review , 2008 .

[75]  N. J. Horan,et al.  Energy optimisation from co-digested waste using a two-phase process to generate hydrogen and methane , 2011 .

[76]  S. Venkata Mohan,et al.  Integration of acidogenic and methanogenic processes for simultaneous production of biohydrogen and methane from wastewater treatment , 2008 .

[77]  Irini Angelidaki,et al.  Extreme thermophilic biohydrogen production from wheat straw hydrolysate using mixed culture fermentation: effect of reactor configuration. , 2010, Bioresource technology.

[78]  M. Ghirardi,et al.  Towards the integration of dark and photo fermentative waste treatment. 1. Hydrogen photoproduction by purple bacterium Rhodobacter capsulatus using potential products of starch fermentation , 2008 .

[79]  P. N. Sarma,et al.  Bio-electrochemical evaluation of fermentative hydrogen production process with the function of feeding pH , 2008 .

[80]  S. M. Raj,et al.  A pilot-scale study of biohydrogen production from distillery effluent using defined bacterial co-culture , 2008 .

[81]  S. Srivastava,et al.  Ni2+-uptake inPseudomonas putida strain S4: a possible role of Mg2+-uptake pump , 2006, Journal of Biosciences.

[82]  P. N. Sarma,et al.  Firmicutes with iron dependent hydrogenase drive hydrogen production in anaerobic bioreactor using d , 2011 .

[83]  P. N. Sarma,et al.  Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate. , 2008, Bioresource technology.

[84]  R. Blondeau,et al.  On the Iron Requirement of Lactobacilli Grown in Chemically Defined Medium , 1998, Current Microbiology.

[85]  Duu-Jong Lee,et al.  Bioaugmented hydrogen production from carboxymethyl cellulose and partially delignified corn stalks using isolated cultures , 2008 .

[86]  Derek R. Lovley,et al.  Potential for Direct Interspecies Electron Transfer in Methanogenic Wastewater Digester Aggregates , 2011, mBio.

[87]  G. Mohanakrishna,et al.  Multiple process integrations for broad perspective analysis of fermentative H2 production from wastewater treatment: Technical and environmental considerations , 2013 .

[88]  A Schievano,et al.  Biohydrogen from thermophilic co-fermentation of swine manure with fruit and vegetable waste: maximizing stable production without pH control. , 2011, Bioresource technology.

[89]  C. Chu,et al.  Seed inocula for biohydrogen production from biodiesel solid residues , 2012 .

[90]  G. Mohanakrishna,et al.  A rapid and simple protocol for evaluating biohydrogen production potential (BHP) of wastewater with simultaneous process optimization , 2012 .

[91]  G. Zheng,et al.  Feasibility of biohydrogen production from tofu wastewater with glutamine auxotrophic mutant of Rhodobacter sphaeroides , 2010 .

[92]  Joo-Hwa Tay,et al.  Biohydrogen production with anaerobic fluidized bed reactors—A comparison of biofilm-based and granule-based systems , 2008 .

[93]  Pretreatment evaluation and its application on palm oil mill effluent for bio-hydrogen enhancement and methanogenic activity repression. , 2009, Pakistan journal of biological sciences : PJBS.

[94]  P. N. Sarma,et al.  Influence of reactor configuration on fermentative hydrogen production during wastewater treatment , 2009 .

[95]  Haijun Yang,et al.  Continuous bio-hydrogen production from citric acid wastewater via facultative anaerobic bacteria , 2006 .

[96]  P. N. Sarma,et al.  Photo-biological hydrogen production by the adopted mixed culture: Data enveloping analysis , 2008 .

[97]  Ruihong Zhang,et al.  Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities , 2007 .

[98]  P. Cañizares,et al.  Electricity production by integration of acidogenic fermentation of fruit juice wastewater and fuel cells , 2012 .

[99]  Chunsheng Qiu,et al.  Extreme-thermophilic biohydrogen production from lignocellulosic bioethanol distillery wastewater wi , 2011 .

[100]  V. Gadhamshetty,et al.  Photoparameters in Photofermentative Biohydrogen Production , 2010 .

[101]  René H. Wijffels,et al.  Photobiological hydrogen production: photochemical e)ciency and bioreactor design , 2002 .

[102]  Chunzhao Liu,et al.  Enhanced coproduction of hydrogen and methane from cornstalks by a three-stage anaerobic fermentation process integrated with alkaline hydrolysis. , 2012, Bioresource technology.

[103]  S. Venkata Mohan,et al.  Self-immobilization of acidogenic mixed consortia on mesoporous material (SBA-15) and activated carbon to enhance fermentative hydrogen production , 2008 .

[104]  Lynne E. Macaskie,et al.  A two-stage, two-organism process for biohydrogen from glucose , 2006 .

[105]  I. Angelidaki,et al.  Biohydrogen production from desugared molasses (DM) using thermophilic mixed cultures immobilized on , 2011 .

[106]  F. Kargı,et al.  Hydrogen gas production from olive mill wastewater by electrohydrolysis with simultaneous COD remova , 2011 .

[107]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .

[108]  Han-Qing Yu,et al.  Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures , 2002 .

[109]  L. J. Rios-Gonza,et al.  Biohydrogen Production by Anaerobic Biofilms from a Pretreated Mixed Microflora , 2010 .

[110]  S. Venkata Mohan,et al.  Optimization and evaluation of fermentative hydrogen production and wastewater treatment processes using data enveloping analysis (DEA) and Taguchi design of experimental (DOE) methodology , 2009 .

[111]  A. Stams,et al.  Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus. , 2003, Biotechnology and bioengineering.

[112]  I. Ntaikou,et al.  Exploitation of olive oil mill wastewater for combined biohydrogen and biopolymers production. , 2009, Bioresource technology.

[113]  Hisham Hafez,et al.  Comparative assessment of single-stage and two-stage anaerobic digestion for the treatment of thin stillage. , 2012, Bioresource technology.

[114]  Jo-Shu Chang,et al.  Engineering strategies for the enhanced photo-H2 production using effluents of dark fermentation processes as substrate , 2010 .

[115]  Chiu-Yue Lin,et al.  A nutrient formulation for fermentative hydrogen production using anaerobic sewage sludge microflora , 2005 .

[116]  Zhi Ping Xu,et al.  Fermentative hydrogen production from fresh leachate in batch and continuous bioreactors. , 2011, Bioresource technology.

[117]  Han-Qing Yu,et al.  From wastewater to bioenergy and biochemicals via two-stage bioconversion processes: a future paradigm. , 2011, Biotechnology advances.

[118]  Lemi Türker,et al.  Hydrogen production and transcriptional analysis of nifD, nifK and hupS genes in Rhodobacter sphaeroides O.U.001 grown in media with different concentrations of molybdenum and iron , 2006 .

[119]  J. Bao,et al.  Photo-fermentation of Rhodobacter sphaeroides for hydrogen production using lignocellulose-derived organic acids , 2010 .

[120]  Jun Hirose,et al.  Hydrogen production by immobilized cells of aciduric Enterobacter aerogenes strain HO-39☆ , 1997 .

[121]  P. N. Sarma,et al.  Microbial diversity analysis of long term operated biofilm configured anaerobic reactor producing bi , 2010 .

[122]  S. Venkata Mohan,et al.  Utilizing acid-rich effluents of fermentative hydrogen production process as substrate for harnessing bioelectricity: An integrative approach , 2010 .

[123]  Lemi Türker,et al.  Photobiological hydrogen production by using olive mill wastewater as a sole substrate source , 2004 .

[124]  Ebru Özgür,et al.  Potential use of thermophilic dark fermentation effluents in photofermentative hydrogen production by Rhodobacter capsulatus , 2010 .

[125]  Yvain Nicolet,et al.  Fe-only hydrogenases: structure, function and evolution. , 2002, Journal of inorganic biochemistry.

[126]  S. Venkata Mohan,et al.  Biohydrogen production from chemical wastewater as substrate by selectively enriched anaerobic mixed consortia: Influence of fermentation pH and substrate composition , 2007 .

[127]  Bruce E Logan,et al.  Inhibition of biohydrogen production by ammonia. , 2006, Water research.

[128]  F. Morsy.  Hydrogen production from acid hydrolyzed molasses by the hydrogen overproducing Escherichia coli str , 2011 .

[129]  David M. Bagley,et al.  Improving the yield from fermentative hydrogen production , 2007, Biotechnology Letters.

[130]  P. Kaparaju,et al.  Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. , 2009, Bioresource technology.

[131]  Y. Oh,et al.  Effect of iron concentration on continuous H2 production using membrane bioreactor , 2009 .

[132]  Guang-qing Liu,et al.  Biohydrogen production from cattle wastewater by enriched anaerobic mixed consortia: influence of fermentation temperature and pH. , 2008, Journal of bioscience and bioengineering.

[133]  C. Chu,et al.  Hydrogen production from mushroom farm waste with a two-step acid hydrolysis process , 2011 .

[134]  P. N. Sarma,et al.  Regulatory influence of CO2 supplementation on fermentative hydrogen production process , 2010 .

[135]  H. Hamelers,et al.  Principle and perspectives of hydrogen production through biocatalyzed electrolysis , 2006 .

[136]  Yu-You Li,et al.  Effect of sludge recirculation on characteristics of hydrogen production in a two-stage hydrogen–methane fermentation process treating food wastes , 2012 .

[137]  K. Seifert,et al.  Brewery wastewaters in photobiological hydrogen generation in presence of Rhodobacter sphaeroides O.U. 001 , 2010 .

[138]  P. N. Sarma,et al.  Acetate and butyrate as substrates for hydrogen production through photo-fermentation: Process optimization and combined performance evaluation ☆ , 2009 .

[139]  Poonsuk Prasertsan,et al.  Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. , 2009, Bioresource technology.

[140]  Chieh-Chen Huang,et al.  Syntrophic co-culture of aerobic Bacillus and anaerobic Clostridium for bio-fuels and bio-hydrogen production , 2008 .

[141]  M. Mandrand,et al.  Antagonistic effect of nickel on the fermentative growth of Escherichia coli K-12 and comparison of nickel and cobalt toxicity on the aerobic and anaerobic growth. , 1994, Environmental health perspectives.

[142]  G. Mohanakrishna,et al.  Predominance of Bacilli and Clostridia in microbial community of biohydrogen producing biofilm sustained under diverse acidogenic operating conditions , 2012 .

[143]  A. Reungsang,et al.  Biological hydrogen production from sweet sorghum syrup by mixed cultures using an anaerobic sequencing batch reactor (ASBR) , 2011 .

[144]  Gaosheng Zhang,et al.  Optimization of initial substrate and pH levels for germination of sporing hydrogen-producing anaerobes in cow dung compost. , 2004, Bioresource technology.

[145]  P. N. Sarma,et al.  Acidogenic fermentation of vegetable based market waste to harness biohydrogen with simultaneous stabilization. , 2009, Bioresource technology.

[146]  H Yokoi,et al.  Microbial hydrogen production from sweet potato starch residue. , 2001, Journal of bioscience and bioengineering.

[147]  P. Carlozzi,et al.  Production of bioH2 by Rhodopseudomonas palustris (strain 6A) grown in pre-treated olive mill waste, under batch or semi-continuous regime , 2010 .

[148]  Chunzhao Liu,et al.  Coproduction of hydrogen and methane via anaerobic fermentation of cornstalk waste in continuous stirred tank reactor integrated with up-flow anaerobic sludge bed. , 2012, Bioresource technology.

[149]  L. Whang,et al.  A two-stage bioprocess for hydrogen and methane production from rice straw bioethanol residues. , 2012, Bioresource technology.

[150]  Hang-sik Shin,et al.  Continuous fermentative hydrogen and methane production from Laminaria japonica using a two-stage fermentation system with recycling of methane fermented effluent , 2012 .

[151]  Tong Zhang,et al.  Effect of ferrous ion on photo heterotrophic hydrogen production by Rhodobacter sphaeroides , 2007 .

[152]  Haijun Yang,et al.  Effect of ferrous iron concentration on anaerobic bio-hydrogen production from soluble starch , 2006 .

[153]  S Venkata Mohan,et al.  Effect of substrate loading rate of chemical wastewater on fermentative biohydrogen production in biofilm configured sequencing batch reactor. , 2008, Bioresource technology.

[154]  Y. Chisti,et al.  Photofermentive production of biohydrogen from oil palm waste hydrolysate , 2012 .

[155]  S. Foster,et al.  Manganese: elemental defence for a life with oxygen. , 2002, Trends in microbiology.

[156]  Q. Hu,et al.  Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. , 2008, The Plant journal : for cell and molecular biology.

[157]  I. Eroglu,et al.  Amelioration of photofermentative hydrogen production from molasses dark fermenter effluent by zeolite-based removal of ammonium ion , 2012 .

[158]  Ufuk Gündüz,et al.  Photosynthetic bacterial growth and productivity under continuous illumination or diurnal cycles with olive mill wastewater as feedstock , 2010 .

[159]  André Bories,et al.  Anaerobic digestion of the vinasses from the fermentation of Agave tequilana Weber to tequila: The effect of pH, temperature and hydraulic retention time on the production of hydrogen and methane , 2009 .

[160]  B. Fang,et al.  Optimization of bio-hydrogen production from biodiesel wastes by Klebsiella pneumoniae. , 2007, Biotechnology journal.

[161]  H. Hamelers,et al.  Performance of single chamber biocatalyzed electrolysis with different types of ion exchange membranes. , 2007, Water research.

[162]  I. Eroglu,et al.  Effect of inactivation of genes involved in ammonium regulation on the biohydrogen production of Rho , 2011 .

[163]  Matthias Schumacher,et al.  Photoproduction of hydrogen by Rhodobacter capsulatus from thermophilic fermentation effluent , 2009, Bioprocess and biosystems engineering.

[164]  C. Lin,et al.  Calcium effect on fermentative hydrogen production in an anaerobic up-flow sludge blanket system. , 2006, Water science and technology : a journal of the International Association on Water Pollution Research.

[165]  Gerasimos Lyberatos,et al.  ADM1-based modeling of methane production from acidified sweet sorghum extract in a two stage process. , 2012, Bioresource technology.

[166]  Jianquan Shen,et al.  Hydrogen production in batch culture of mixed bacteria with sucrose under different iron concentrations , 2005 .

[167]  S. Venkata Mohan,et al.  Effluents with soluble metabolites generated from acidogenic and methanogenic processes as substrate for additional hydrogen production through photo-biological process , 2009 .

[168]  Alissara Reungsang,et al.  Optimization of biohydrogen production from sweet sorghum syrup using statistical methods , 2010 .

[169]  D. L. Hawkes,et al.  Enhancement of hydrogen production from glucose by nitrogen gas sparging. , 2000 .

[170]  Zhaobo Chen,et al.  Effects of different pretreatment methods on fermentation types and dominant bacteria for hydrogen production , 2008 .

[171]  Mi‐Sun Kim,et al.  Bacterial community analyses by pyrosequencing in dark fermentative H2-producing reactor using organic wastes as a feedstock , 2012 .

[172]  Bruce E Logan,et al.  Sustainable and efficient biohydrogen production via electrohydrogenesis , 2007, Proceedings of the National Academy of Sciences.

[173]  M. Maguire,et al.  Emerging themes in manganese transport, biochemistry and pathogenesis in bacteria. , 2003, FEMS microbiology reviews.

[174]  J. Heijnen,et al.  Importance of bacterial storage polymers in bioprocesses , 1997 .

[175]  Biswarup Sen,et al.  Mesophilic fermentative hydrogen production from sago starch-processing wastewater using enriched mixed cultures , 2012 .

[176]  Mi‐Sun Kim,et al.  Continuous hydrogen production from tofu processing waste using anaerobic mixed microflora under thermophilic conditions , 2011 .

[177]  S. Singh,et al.  Biohydrogen Production from Cheese Whey Wastewater in a Two-Step Anaerobic Process , 2012, Applied Biochemistry and Biotechnology.

[178]  S Venkata Mohan,et al.  Biohydrogen production from chemical wastewater treatment in biofilm configured reactor operated in periodic discontinuous batch mode by selectively enriched anaerobic mixed consortia. , 2007, Water research.

[179]  Jianlong Wang,et al.  FACTORS INFLUENCING FERMENTATIVE HYDROGEN PRODUCTION: A REVIEW , 2009 .

[180]  P. Hallenbeck.  Microbial paths to renewable hydrogen production , 2011 .

[181]  Chyi-How Lay,et al.  Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora , 2004 .

[182]  Sang-Eun Oh,et al.  Hydrogen and methane production from swine wastewater using microbial electrolysis cells. , 2009, Water research.

[183]  K. Sasikala,et al.  Anoxygenic Phototrophic Bacteria: Physiology and Advances in Hydrogen Production Technology , 1993 .

[184]  Jo‐Shu Chang,et al.  Photo fermentative hydrogen production using dominant components (acetate, lactate, and butyrate) in , 2011 .

[185]  S. Mohan,et al.  Fatty acid rich effluent from acidogenic biohydrogen reactor as substrate for lipid accumulation in heterotrophic microalgae with simultaneous treatment. , 2012 .

[186]  M. Nienoord,et al.  Energy aspects of biological hydrogen production in high rate bioreactors operated in the thermophilic temperature range , 2002 .

[187]  J. Puhakka,et al.  Enhancement of anaerobic hydrogen production by iron and nickel , 2010 .

[188]  R. Dinsdale,et al.  Fermentative biohydrogen production systems integration. , 2011, Bioresource technology.

[189]  B. Rittmann.  Opportunities for renewable bioenergy using microorganisms. , 2008, Biotechnology and bioengineering.

[190]  Marcelo Zaiat,et al.  Potential to produce biohydrogen from various wastewaters , 2010 .

[191]  Sanjay K. S. Patel,et al.  Exploitation of defined bacterial cultures for production of hydrogen and polyhydroxybutyrate from pea-shells. , 2012 .

[192]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[193]  T. Noike,et al.  Effect of iron concentration on hydrogen fermentation. , 2001, Bioresource technology.

[194]  Hisham Hafez,et al.  An integrated system for hydrogen and methane production during landfill leachate treatment , 2010 .

[195]  A. Ogino,et al.  Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora. , 2009, Journal of bioscience and bioengineering.

[196]  A. Lanzini,et al.  Bio-hydrogen production from organic wastes in a pilot plant reactor and its use in a SOFC , 2011 .

[197]  J. Lay,et al.  Co-culture of Clostridium beijerinckii L9, Clostridium butyricum M1 and Bacillus thermoamylovorans B , 2011 .

[198]  G. Mohanakrishna,et al.  Biohydrogen Production from Industrial Effluents , 2011 .

[199]  Chun-Hsiung Hung,et al.  Feasible pretreatment of textile wastewater for dark fermentative hydrogen production , 2012 .

[200]  Jianquan Shen,et al.  Effect of temperature and iron concentration on the growth and hydrogen production of mixed bacteria , 2006 .

[201]  T. Keskin,et al.  Photofermentative hydrogen production from wastes. , 2011, Bioresource technology.

[202]  S. Venkata Mohan,et al.  Harnessing of biohydrogen from wastewater treatment using mixed fermentative consortia: Process evaluation towards optimization , 2009 .

[203]  Jan A. Oleszkiewicz,et al.  Stimulation and inhibition of anaerobic processes by heavy metals—A review , 1990 .

[204]  S Venkata Mohan,et al.  Fermentative effluents from hydrogen producing bioreactor as substrate for poly(beta-OH) butyrate production with simultaneous treatment: an integrated approach. , 2010, Bioresource technology.

[205]  Shunsaku Ueda,et al.  Hydrogen production as a novel process of wastewater treatment—studies on tofu wastewater with entrapped R. sphaeroides and mutagenesis , 2002 .

[206]  E. Madsen.  Environmental Microbiology: From Genomes to Biogeochemistry , 2008 .

[207]  Xian-Yang Shi,et al.  Optimization of conditions for hydrogen production from brewery wastewater by anaerobic sludge using desirability function approach , 2010 .

[208]  N. Ren,et al.  Enhanced cellulosic hydrogen production from lime-treated cornstalk wastes using thermophilic anaerobic microflora , 2012 .

[209]  P. Rangsunvigit,et al.  Hydrogen production from alcohol distillery wastewater containing high potassium and sulfate using a , 2011 .

[210]  H. Argun,et al.  Photo-fermentative hydrogen gas production from dark fermentation effluent of ground wheat solution: Effects of light source and light intensity , 2010 .

[211]  Chimei Lee,et al.  Hydrogen production by Rhodopseudomonas palustris WP 3-5 in a serial photobioreactor fed with hydrogen fermentation effluent. , 2011, Bioresource technology.

[212]  P. N. Sarma,et al.  Simultaneous biohydrogen production and wastewater treatment in biofilm configured anaerobic periodic discontinuous batch reactor using distillery wastewater , 2008 .

[213]  L. Celis,et al.  Different start-up strategies to enhance biohydrogen production from cheese whey in UASB reactors , 2012 .

[214]  V. Himabindu,et al.  Biological hydrogen production from probiotic wastewater as substrate by selectively enriched anaerobic mixed microflora , 2009 .

[215]  B. Wang,et al.  Fermentative hydrogen production from molasses wastewater in a continuous mixed immobilized sludge reactor. , 2012, Bioresource technology.

[216]  H. Drake,et al.  Nickel transport by the thermophilic acetogen Acetogenium kivui , 1989, Applied and environmental microbiology.

[217]  X Vernede,et al.  The crystal structure of a reduced [NiFeSe] hydrogenase provides an image of the activated catalytic center. , 1999, Structure.

[218]  Jianlong Wang,et al.  Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge , 2008 .

[219]  M. Seibert,et al.  Towards the integration of dark- and photo-fermentative waste treatment. 3. Potato as substrate for sequential dark fermentation and light-driven H2 production , 2010 .

[220]  F. Kargı,et al.  Bio-hydrogen production from acid hydrolyzed waste ground wheat by dark fermentation , 2011 .

[221]  Jianlong Wang,et al.  Influence of Ni(2+) concentration on biohydrogen production. , 2008, Bioresource technology.

[222]  Dipankar Ghosh,et al.  Advances in fermentative biohydrogen production: the way forward? , 2009, Trends in biotechnology.

[223]  Hang-Sik Shin,et al.  Effect of initial pH independent of operational pH on hydrogen fermentation of food waste. , 2011, Bioresource technology.

[224]  Chiu-Yue Lin,et al.  Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora , 2004 .

[225]  Zhaobo Chen,et al.  Removal of organic pollutants and analysis of MLSS-COD removal relationship at different HRTs in a submerged membrane bioreactor , 2005 .

[226]  H. Argun,et al.  Bio-hydrogen production from acid hydrolyzed wheat starch by photo-fermentation using different Rhodobacter sp , 2009 .

[227]  S. Mohan,et al.  Pretreatment of Biocatalyst as Viable Option for Sustained Production of Biohydrogen from Wastewater Treatment , 2012 .

[228]  K Chandrasekhar,et al.  Influence of carbohydrates and proteins concentration on fermentative hydrogen production using canteen based waste under acidophilic microenvironment. , 2011, Journal of biotechnology.

[229]  M. Yücel,et al.  HYDROGEN PRODUCTION BY R. CAPSULATUS ON DARK FERMENTER EFFLUENT OF POTATO STEAM PEEL HYDROLYSATE , 2009 .

[230]  Qi Zhou,et al.  Evaluation of pretreatment methods on mixed inoculum for both batch and continuous thermophilic biohydrogen production from cassava stillage. , 2010, Bioresource technology.

[231]  Chin-Chao Chen,et al.  Biohydrogen and biomethane from water hyacinth ( Eichhornia crassipes) fermentation: Effects of subs , 2011 .

[232]  P. N. Sarma,et al.  Enhancing biohydrogen production from chemical wastewater treatment in anaerobic sequencing batch biofilm reactor (AnSBBR) by bioaugmenting with selectively enriched kanamycin resistant anaerobic mixed consortia , 2007 .

[233]  M. Jacobson,et al.  Transcriptional regulation of nitrogen fixation by molybdenum in Azotobacter vinelandii , 1986, Journal of bacteriology.

[234]  Bruce E. Logan,et al.  Hydrogen production from cellulose in a two-stage process combining fermentation and electrohydrogenesis , 2009 .

[235]  S. Venkata Mohan,et al.  Microalgal community and their growth conditions influence biohydrogen production during integration , 2011 .

[236]  Liang Guo,et al.  Effect of S-TE (solubilization by thermophilic enzyme) digestion conditions on hydrogen production from waste sludge. , 2012, Bioresource technology.

[237]  P. N. Sarma,et al.  Anaerobic biohydrogen production from dairy wastewater treatment in sequencing batch reactor (AnSBR) : Effect of organic loading rate , 2007 .

[238]  Debabrata Das,et al.  Improvement of fermentative hydrogen production: various approaches , 2004, Applied Microbiology and Biotechnology.

[239]  A. Morby,et al.  Zn(II) metabolism in prokaryotes. , 2003, FEMS microbiology reviews.

[240]  F. Kargı,et al.  Bio-hydrogen production by photo-fermentation of dark fermentation effluent with intermittent feeding and effluent removal , 2010 .

[241]  I. Eroglu,et al.  Biohydrogen production from beet molasses by sequential dark and photofermentation , 2010 .

[242]  S. Babel,et al.  Effects of pretreatment methods on cassava wastewater for biohydrogen production optimization , 2012 .

[243]  Jo-Shu Chang,et al.  Perspectives on cultivation strategies and photobioreactor designs for photo-fermentative hydrogen production. , 2011, Bioresource technology.

[244]  R. Gunsalus,et al.  Syntrophy in anaerobic global carbon cycles. , 2009, Current opinion in biotechnology.

[245]  S. Tanisho,et al.  Effect of CO2 removal on hydrogen production by fermentation , 1998 .

[246]  N. Ren,et al.  Enhanced photo-H2 production of R. faecalis RLD-53 by separation of CO2 from reaction system. , 2009, Bioresource technology.

[247]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .

[248]  D. Ahmad,et al.  Biohydrogen generation from palm oil mill effluent using anaerobic contact filter , 2006 .

[249]  Hang-Sik Shin,et al.  Continuous fermentative hydrogen production from coffee drink manufacturing wastewater by applying UASB reactor , 2010 .

[250]  Tsuyoshi Imai,et al.  Biohydrogen production from cassava starch processing wastewater by thermophilic mixed cultures , 2011 .

[251]  I. Eroglu,et al.  Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides , 2002 .

[252]  A. Pandey,et al.  Optimization of hydrogen production by Rhodobacter sphaeroides NMBL-01 , 2012 .

[253]  P. N. Sarma,et al.  Enhancing biohydrogen production through sewage supplementation of composite vegetable based market waste , 2010 .

[254]  Peter Seto,et al.  Eliminating methanogenic activity in hydrogen reactor to improve biogas production in a two-stage anaerobic digestion process co-digesting municipal food waste and sewage sludge. , 2011, Bioresource technology.

[255]  Godfrey Kyazze,et al.  Continuous dark fermentative hydrogen production by mesophilic microflora: principles and progress , 2007 .