BMP-2 and miR-29c in osteosarcoma tissues on proliferation and invasion of osteosarcoma cells

Expression of bone morphogenetic protein (BMP)-2 and microRNA (miR)-29c in osteosarcoma tissues and effects on proliferation and invasion of osteosarcoma cells were investigated. A retrospective analysis of 75 patients with osteosarcoma who underwent surgery in Tianjin Baodi Hospital from May 2013 to June 2017 was conducted. A total of 75 osteosarcoma tissues and 51 normal paraneoplastic tissues were collected. RT-PCR was used to compare the expression difference of BMP-2 and miR-29c. miR-29c mimics (experimental group A) and BMP-2 siRNA plasmid (experimental group B) were transfected into human osteosarcoma cells MG-63, respectively. The transfected cells were divided into miRNA negative control (miR-NC) group (cells transfected with miR-negative control), siRNA negative control group (cells transfected with non-silent siRNA) and blank group (cells without any transfection). MTT assay was used to detect cell proliferation in each group at different time periods. Transwell insert was used to detect invasion of cells in vitro. The relative expression of BMP-2 in osteosarcoma tissue was significantly higher than that in paraneoplastic tissue (P<0.05). Τhe relative expression of miR-29c in osteosarcoma tissue was significantly lower than that in paraneoplastic tissue (P<0.05). The cell survival rates in experimental groups A and B were significantly lower than those in the blank, miR-NC negative control and siRNA negative control groups on day 5 (P<0.05). The number of cell transmembranes in experimental groups A and B was significantly lower than those in the blank, miR-NC negative control and siRNA negative control groups (P<0.05). BMP-2 is over-expressed in osteosarcoma tissues, and miR-29c is under-expressed in osteosarcoma tissues. Interfering with the expression of BMP-2 and overexpression of miR-29c can inhibit the proliferation and invasion of osteosarcoma cells, indicating that BMP-2 and miR-29c may be involved in the regulation of proliferation and metastasis of osteosarcoma cells and could be used as new molecular target markers for the diagnosis and treatment of osteosarcoma.

[1]  Liankun Sun,et al.  p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2. , 2017, Biochemical and biophysical research communications.

[2]  Yong Mou,et al.  MicroRNA-184 Modulates Doxorubicin Resistance in Osteosarcoma Cells by Targeting BCL2L1 , 2016, Medical science monitor : international medical journal of experimental and clinical research.

[3]  Wei Li,et al.  Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis , 2016, Journal of bone oncology.

[4]  Y. Qi,et al.  MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R , 2016, Cellular Physiology and Biochemistry.

[5]  Bin Zhang,et al.  MicroRNA-497 inhibits cell proliferation, migration, and invasion by targeting AMOT in human osteosarcoma cells , 2016, OncoTargets and therapy.

[6]  Guangya Wang,et al.  IGF1 3′UTR functions as a ceRNA in promoting angiogenesis by sponging miR-29 family in osteosarcoma , 2016, Journal of Molecular Histology.

[7]  G. Melino,et al.  P53 functional abnormality in mesenchymal stem cells promotes osteosarcoma development , 2016, Cell Death and Disease.

[8]  P. Liu,et al.  Mechanism of BMP9 promotes growth of osteosarcoma mediated by the Notch signaling pathway , 2015, Oncology letters.

[9]  Megan E Anderson.  Update on Survival in Osteosarcoma. , 2016, The Orthopedic clinics of North America.

[10]  David M. Thomas,et al.  A Genome-Wide Scan Identifies Variants in NFIB Associated with Metastasis in Patients with Osteosarcoma. , 2015, Cancer discovery.

[11]  Le Chang,et al.  Review of microRNA in osteosarcoma and chondrosarcoma , 2015, Medical Oncology.

[12]  H. Zhang,et al.  Reduction of miR-29c enhances pancreatic cancer cell migration and stem cell-like phenotype , 2014, Oncotarget.

[13]  A. Giordano,et al.  MicroRNA-29b-1 impairs in vitro cell proliferation, self-renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells , 2014, International journal of oncology.

[14]  Jun Fang,et al.  Prognostic value of the microRNA-29 family in patients with primary osteosarcomas , 2014, Medical Oncology.

[15]  X. Liu,et al.  Expression of miR-29c, miR-93, and miR-429 as Potential Biomarkers for Detection of Early Stage Non-Small Lung Cancer , 2014, PloS one.

[16]  Kangkang Liu,et al.  Flouride Promotes Viability and Differentiation of Osteoblast-Like Saos-2 Cells Via BMP/Smads Signaling Pathway , 2013, Biological Trace Element Research.

[17]  Ying Sun,et al.  MiR-29c suppresses invasion and metastasis by targeting TIAM1 in nasopharyngeal carcinoma. , 2013, Cancer letters.

[18]  Kevin B. Jones,et al.  miRNA signatures associate with pathogenesis and progression of osteosarcoma. , 2012, Cancer research.

[19]  R. Stanton,et al.  Sclerostin expression is induced by BMPs in human Saos-2 osteosarcoma cells but not via direct effects on the sclerostin gene promoter or ECR5 element. , 2011, Bone.

[20]  Chia-Ying Lin,et al.  BMP-2 inhibits the tumorigenicity of cancer stem cells in human osteosarcoma OS99-1 cell line , 2011, Cancer biology & therapy.

[21]  J. Chi,et al.  MicroRNA expression in chronic lymphocytic leukaemia , 2009, British journal of haematology.

[22]  Benjamin Haibe-Kains,et al.  microRNA-29c and microRNA-223 down-regulation has in vivo significance in chronic lymphocytic leukemia and improves disease risk stratification. , 2009, Blood.

[23]  P. Picci,et al.  Neoadjuvant Chemotherapy for Osteosarcoma of the Extremities in Preadolescent Patients: The Rizzoli Institute Experience , 2008, Journal of pediatric hematology/oncology.

[24]  S. Ferrari,et al.  Poor prognosis osteosarcoma: new therapeutic approach , 2008, Bone Marrow Transplantation.

[25]  Chengla Yi,et al.  Relationship between the expression of MTA-1 gene and the metastasis and invasion in human osteosarcoma. , 2005, Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban.

[26]  P. Picci,et al.  Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells. , 2002, International journal of oncology.

[27]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[28]  S. Ferrari,et al.  Local and systemic control for osteosarcoma of the extremity treated with neoadjuvant chemotherapy and limb salvage surgery: the Rizzoli experience. , 2000, Oncology reports.

[29]  M. Ladanyi,et al.  Expression of bone morphogenetic proteins and receptors in sarcomas. , 1999, Clinical orthopaedics and related research.

[30]  Liankun Sun,et al.  p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2. , 2017, Biochemical and biophysical research communications.

[31]  Yong Mou,et al.  MicroRNA-184 Modulates Doxorubicin Resistance in Osteosarcoma Cells by Targeting BCL2L1 , 2016, Medical science monitor : international medical journal of experimental and clinical research.

[32]  Wei Li,et al.  Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis , 2016, Journal of bone oncology.

[33]  Y. Qi,et al.  MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R , 2016, Cellular Physiology and Biochemistry.

[34]  Bin Zhang,et al.  MicroRNA-497 inhibits cell proliferation, migration, and invasion by targeting AMOT in human osteosarcoma cells , 2016, OncoTargets and therapy.

[35]  Guangya Wang,et al.  IGF1 3′UTR functions as a ceRNA in promoting angiogenesis by sponging miR-29 family in osteosarcoma , 2016, Journal of Molecular Histology.

[36]  G. Melino,et al.  P53 functional abnormality in mesenchymal stem cells promotes osteosarcoma development , 2016, Cell Death and Disease.

[37]  P. Liu,et al.  Mechanism of BMP9 promotes growth of osteosarcoma mediated by the Notch signaling pathway , 2015, Oncology letters.

[38]  Megan E Anderson.  Update on Survival in Osteosarcoma. , 2016, The Orthopedic clinics of North America.

[39]  David M. Thomas,et al.  A Genome-Wide Scan Identifies Variants in NFIB Associated with Metastasis in Patients with Osteosarcoma. , 2015, Cancer discovery.

[40]  Le Chang,et al.  Review of microRNA in osteosarcoma and chondrosarcoma , 2015, Medical Oncology.

[41]  H. Zhang,et al.  Reduction of miR-29c enhances pancreatic cancer cell migration and stem cell-like phenotype , 2014, Oncotarget.

[42]  A. Giordano,et al.  MicroRNA-29b-1 impairs in vitro cell proliferation, self-renewal and chemoresistance of human osteosarcoma 3AB-OS cancer stem cells , 2014, International journal of oncology.

[43]  Jun Fang,et al.  Prognostic value of the microRNA-29 family in patients with primary osteosarcomas , 2014, Medical Oncology.

[44]  X. Liu,et al.  Expression of miR-29c, miR-93, and miR-429 as Potential Biomarkers for Detection of Early Stage Non-Small Lung Cancer , 2014, PloS one.

[45]  Kangkang Liu,et al.  Flouride Promotes Viability and Differentiation of Osteoblast-Like Saos-2 Cells Via BMP/Smads Signaling Pathway , 2013, Biological Trace Element Research.

[46]  Ying Sun,et al.  MiR-29c suppresses invasion and metastasis by targeting TIAM1 in nasopharyngeal carcinoma. , 2013, Cancer letters.

[47]  Kevin B. Jones,et al.  miRNA signatures associate with pathogenesis and progression of osteosarcoma. , 2012, Cancer research.

[48]  R. Stanton,et al.  Sclerostin expression is induced by BMPs in human Saos-2 osteosarcoma cells but not via direct effects on the sclerostin gene promoter or ECR5 element. , 2011, Bone.

[49]  Chia-Ying Lin,et al.  BMP-2 inhibits the tumorigenicity of cancer stem cells in human osteosarcoma OS99-1 cell line , 2011, Cancer biology & therapy.

[50]  J. Chi,et al.  MicroRNA expression in chronic lymphocytic leukaemia , 2009, British journal of haematology.

[51]  Benjamin Haibe-Kains,et al.  microRNA-29c and microRNA-223 down-regulation has in vivo significance in chronic lymphocytic leukemia and improves disease risk stratification. , 2009, Blood.

[52]  P. Picci,et al.  Neoadjuvant Chemotherapy for Osteosarcoma of the Extremities in Preadolescent Patients: The Rizzoli Institute Experience , 2008, Journal of pediatric hematology/oncology.

[53]  S. Ferrari,et al.  Poor prognosis osteosarcoma: new therapeutic approach , 2008, Bone Marrow Transplantation.

[54]  Liu Xinzhi,et al.  Relationship between the expression of MTA-1 gene and the metastasis and invasion in human osteosarcoma , 2008, Journal of Huazhong University of Science and Technology [Medical Sciences].

[55]  P. Picci,et al.  Seven BMPs and all their receptors are simultaneously expressed in osteosarcoma cells. , 2002, International journal of oncology.

[56]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[57]  S. Ferrari,et al.  Local and systemic control for osteosarcoma of the extremity treated with neoadjuvant chemotherapy and limb salvage surgery: the Rizzoli experience. , 2000, Oncology reports.

[58]  M. Ladanyi,et al.  Expression of bone morphogenetic proteins and receptors in sarcomas. , 1999, Clinical orthopaedics and related research.