Complete mitochondrial DNA sequence of the Japanese flying fish Cypselurus hiraii

In this study, to develop a technique that enables authentication of processed seafood, the complete nucleotide sequence of the mitochondrial genome for the Japanese flying fish Cypselurus hiraii was determined. Three segments spanning the entire genome were amplified using polymerase chain reaction, and products were subsequently used as templates for direct sequencing with 60 primers. The genome (16 528 base pairs) was found to contain the same 37 genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrate mitochondrial genomes, with the gene order being identical to that typical of vertebrates. A major noncoding region between the tRNAPro and tRNAPhe genes (868 base pairs) appears to be the control (D-loop) region, as it has several conserved blocks characteristic of control regions.

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