The ecological footprint (EF) is a sustainability indicator which provides a referential framework for the analysis of human demand for bioproductivity, including energy issues. Even though footprint analysis was mainly thought from a territory point of view, it can be also applied to companies and, in general, organizations. In this paper, we develop a general description of the indicator, pointing out its objectives and calculation method. In addition, footprint’s strengths and weaknesses are also stated. We also remark the particularities of organization’s footprints, applying the indicator to two Galician fishing companies. Results show important differences between the studied companies, being able to identify energy and fish feed consumptions as the main responsible of both the EF and the differences between companies.