Compositional differences of Bojungikgi-tang decoctions using pressurized or non-pressurized extraction methods with variable extraction times

ABSTRACT Objectives : In other to determine the optimal extraction conditions, the v arious Bojungikgi-tang (BJIGT) decoctions prepared by different pressure levels and different extraction times were compared and evaluated in terms of the extract yield and the total soluble solid content. Methods : Decoctions were prepared by the pressure levels of 0 (non-pressurized) and 1 kgf/cm 2 (pressurized) for 60, 120 and 180 min. The extract yield and the total solubl e solids content of decoctions were measured, and the amounts of the reference compounds in decoctions were i nvestigated by the analysis using high performance liquid chromatography. Results : The extract yield and the total soluble solid content were higher in decoctions extracted by the pressurized method than those from decoction with non-pressuriz ed method. The patterns of yield and contents showed a proportional increase to the extraction time. In analysis of the linear regression for four reference compounds such as liquiritin, nodakenin, hesperidin, and glycyr rhizin, the good linearity with the correlation coefficient more than 0.9999 was observed. The highest contents for four reference compounds were observed at 180 min of both the pressurized method and the non-pressurized method.Conclusions : This study suggests that the pressure in extraction method and the extraction time affect the compositional constituents in BJIGT decoctions. The extraction time of 180 min could be chosen in both pressurized and non-pressurized method as optimal extraction co ndition.Key words : Bojungikgi-tang, extraction condition, extraction yield, total soluble solids content, reference compounds, regression analysis

[1]  H. Yang,et al.  Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a high-performance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer , 2013, Pharmacognosy magazine.

[2]  Yuko Saito,et al.  Extraction Efficiency of Shosaikoto (Xiaochaihu Tang) and Investigation of the Major Constituents in the Residual Crude Drugs , 2012, Evidence-based complementary and alternative medicine : eCAM.

[3]  K. Hamid,et al.  Study on important factors affecting the extraction of momordica charantia using pressurized boiling system , 2011, 2011 IEEE Symposium on Business, Engineering and Industrial Applications (ISBEIA).

[4]  Bum-chun Lee,et al.  Antioxidative activities and composition analysis of Pinus densiflora root by ultra high pressure extraction , 2011 .

[5]  Hailiang Jiang,et al.  Optimization of extraction of evodiamine and rutaecarpine from fruit of Evodia rutaecarpa using modified supercritical CO(2). , 2010, Journal of chromatography. A.

[6]  Qiang Wang,et al.  Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology. , 2010 .

[7]  Mouming Zhao,et al.  Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction. , 2010, Journal of pharmaceutical and biomedical analysis.

[8]  Mouming Zhao,et al.  Application of ultrasonication or high-pressure extraction of flavonoids from litchi fruit pericarp. , 2009 .

[9]  Y. Ohta,et al.  Comparative study of chemical constituents of the traditional medicine hochuekkito by LC-MS with multivariate statistical analysis , 2009 .

[10]  Shouqin Zhang,et al.  Effects of ultrahigh pressure extraction conditions on yields and antioxidant activity of ginsenoside from ginseng , 2009 .

[11]  Mouming Zhao,et al.  Effects of high pressure extraction on the extraction yield, total phenolic content and antioxidant activity of longan fruit pericarp , 2009 .

[12]  E. Kwak.  Development of Brown Colored Rice Tea with High GABA Content , 2010 .

[13]  Lin Jiashou.  Study on effective components of Huanglian Jiedu Decoction in different decocting condition , 2010 .

[14]  김지혜,et al.  Changes in Effective Components of Ginseng by Puffing , 2008 .

[15]  Lee Yong-bum,et al.  Study on the most effective Yinqiao San by the GC(Gas Chromatography) , 2006 .