The aim of this study was to describe biology teachers’ Technological Pedagogical Content Knowledge (TPCK) at Public Senior High School in Pekanbaru. This study held on November 2013 until March 2014. The sample of this study was 33 biology teachers at Public Senior High School in Pekanbaru that selected by random sampling technique. This study conducted with closed-ended and open-ended questionnaire. The closed-ended questionnaire consists of 35 items and is 7 subdomains. The subdomains are Technological Knowledge (TK), Pedagogical Knowledge (PK), Content Knowledge (CK), Technological Pedagogical Knowledge (TPK), Technological Content Knowledge (TCK), Pedagogical Content Knowledge (PCK) and Technological Pedagogical Content Knowledge (TPCK). The closed-ended questionnaire items is valid and reliable. Descriptive study was implemented in this study. The result showed that biology teachers’ TK at Public Senior High School in Pekanbaru was good enough (M=3,38), PK was good (M=4,05), CK was good (M=3,92), TPK was good (M=3,70), TCK was good (M=3,70), PCK was very good (M=4,26) and TPCK was good (M=3,94). An overall, biology teachers’ TPCK was good (M=3,72). That result indicated that biology teacher at Public Senior High School in Pekanbaru could implement their technology knowledge in biology learning. Keywords: Technological Pedagogical Content Knowledge, Senior High School, Biology Teacher
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