Interactive comment on “ Temperature thresholds for Arctic plankton community metabolism : an experimental assessment ” by J . M . Holding

a sufficient number of parameters associated with the plankton community to unambiguously evaluate the community response to temperature. For example, the authors don’t provide any information about nutrient concentrations. There is no mention of nutrient additions so I assume that the populations had to exist on whatever nutrients were available at the time of collection. Given a 15-day incubation period, it is very likely that nutrients in some of the treatments may have been exhausted. Is it possible that this might explain the reduction in chlorophyll in the 9 and 10 degree treatments? We don’t know because nutrient data were not presented. In addition, phytoplankton biomass was only characterized using measurements of chlorophyll and heterotrophic biomass wasn’t characterized at all.’

[1]  Núria Marbà,et al.  Tipping Elements in the Arctic Marine Ecosystem , 2012, AMBIO.

[2]  Dag Slagstad,et al.  Primary production and climatic variability in the European sector of the Arctic Ocean prior to 2007: preliminary results , 2010, Polar Biology.

[3]  C. Duarte,et al.  Changes in Arctic marine bacterial carbon metabolism in response to increasing temperature , 2010, Polar Biology.

[4]  R. Spencer,et al.  Glaciers as a source of ancient and labile organic matter to the marine environment , 2009, Nature.

[5]  A. Hall,et al.  September sea-ice cover in the Arctic Ocean projected to vanish by 2100 , 2009 .

[6]  Kevin R. Arrigo,et al.  Impact of a shrinking Arctic ice cover on marine primary production , 2008 .

[7]  D. O. Hessen,et al.  Light and spectral properties as determinants of C:N:P-ratios in phytoplankton , 2008 .

[8]  Dag Slagstad,et al.  Impact of climatic change on the biological production in the Barents Sea , 2008 .

[9]  Wolfgang Lucht,et al.  Tipping elements in the Earth's climate system , 2008, Proceedings of the National Academy of Sciences.

[10]  R. Kerr Is Battered Arctic Sea Ice Down For the Count? , 2007, Science.

[11]  A. Lopez-Urrutia,et al.  Resource limitation of bacterial production distorts the temperature dependence of oceanic carbon cycling. , 2007, Ecology.

[12]  K. Trenberth,et al.  Observations: Surface and Atmospheric Climate Change , 2007 .

[13]  Marika M. Holland,et al.  Future abrupt reductions in the summer Arctic sea ice , 2006 .

[14]  P. White,et al.  The effect of temperature and algal biomass on bacterial production and specific growth rate in freshwater and marine habitats , 1991, Microbial Ecology.

[15]  Marit Reigstad,et al.  Modelling the ecosystem dynamics of the Barents Sea including the marginal ice zone: II. Carbon flux and interannual variability , 2006 .

[16]  Threshold of gross primary production for planktonic metabolic balance in the Southern Ocean: An experimental test , 2005 .

[17]  William K. W. Li,et al.  Macroecological limits of heterotrophic bacterial abundance in the ocean , 2004 .

[18]  E. Sakshaug Primary and Secondary Production in the Arctic Seas , 2004 .

[19]  Ursula Schauer,et al.  Atlantic Water flow through the Barents and Kara Seas , 2002 .

[20]  James H. Brown,et al.  Effects of Size and Temperature on Metabolic Rate , 2001, Science.

[21]  W. Wiebe,et al.  Temperature and substrates as interactive limiting factors for marine heterotrophic bacteria , 2001 .

[22]  Shalina,et al.  Satellite Evidence for an Arctic Sea Ice Cover in Transformation. , 1999, Science.

[23]  C. Duarte,et al.  The CO2 balance of unproductive aquatic ecosystems , 1998, Science.

[24]  C. Duarte,et al.  Role of experimental approaches in marine microbial ecology , 1997 .

[25]  B. Ådlandsvik,et al.  Water fluxes through the Barents Sea , 1997 .

[26]  D. Pauly,et al.  Primary production required to sustain global fisheries , 1995, Nature.

[27]  P. Morin,et al.  Precise shipboard determination of dissolved oxygen (Winkler procedure) for productivity studies with a commercial system1 , 1988 .

[28]  James H. Carpenter,et al.  THE ACCURACY OF THE WINKLER METHOD FOR DISSOLVED OXYGEN ANALYSIS1 , 1965 .