Establishment of eosinophilic sublines from human promyelocytic leukemia (HL-60) cells: demonstration of multipotentiality and single-lineage commitment of HL-60 stem cells.

Recent observations indicating that the HL-60 human acute promyelocytic leukemia cell line contains a minor eosinophil population in addition to neutrophil and mononuclear phagocyte progenitors suggest the multipotentiality of HL-60 stem cells. In order to clarify multilineage differentiation and commitment to single-lineage progenitors we analyzed HL-60 colonies formed in methylcellulose. In an HL-60 parent line with a relatively high eosinophil content (5.5%), 36% of the spontaneous colonies consisted partly or wholly of eosinophilic cells. After two rounds of subcloning in methylcellulose, two eosinophilic sublines and two neutrophilic sublines were established. These lines have been in continuous liquid culture for more than four months, and they show stable single-lineage differentiation. Purified biosynthetic GM-CSF, which stimulates normal CFU-GM and CFU-EO, induced monocytic differentiation but no eosinophilic differentiation in the neutrophilic sublines and no neutrophilic or monocytic differentiation in the eosinophilic sublines. These observations indicate that HL-60 stem cells are multipotent and capable of spontaneous commitment to single-lineage progenitors. The eosinophilic HL-60 sublines should facilitate studies on the production and function of human eosinophils and the single-lineage sublines will allow further analysis of leukemic cell differentiation and stem cell commitment.

[1]  M. Tomonaga,et al.  High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M. Tomonaga,et al.  Biosynthetic (recombinant) human granulocyte-macrophage colony-stimulating factor: effect on normal bone marrow and leukemia cell lines. , 1986, Blood.

[3]  E. Brown,et al.  Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. , 1985, Science.

[4]  S. Fischkoff,et al.  Switch in differentiative response to maturation inducers of human promyelocytic leukemia cells by prior exposure to alkaline conditions. , 1985, Cancer research.

[5]  H. Sakamaki,et al.  Specific production of eosinophil colony stimulating factor from sensitized T cells from a patient with allergic eosinophilia , 1985, British journal of haematology.

[6]  H. Koeffler,et al.  Study of differentiation of fresh myelogenous leukemic cells by compounds that induce a human promyelocytic leukemic line (HL-60) to differentiate. , 1985, Leukemia research.

[7]  D. Golde,et al.  Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. , 1984, Science.

[8]  H. Koeffler,et al.  Gamma-interferon induces expression of the HLA-D antigens on normal and leukemic human myeloid cells. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Testa,et al.  Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60 , 1984, The Journal of experimental medicine.

[10]  I. Olsson,et al.  Isolation and characterization of a T lymphocyte-derived differentiation inducing factor for the myeloid leukemic cell line HL-60. , 1984, Blood.

[11]  J. Griffin,et al.  Effect of ara-A on differentiation and proliferation of HL-60 cells. , 1984, Leukemia research.

[12]  H. Koeffler.  Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications , 1983 .

[13]  N. Nicola,et al.  Selective stimulation by mouse spleen cell conditioned medium of human eosinophil colony formation. , 1983, Blood.

[14]  D. Metcalf,et al.  Clonal analysis of the response of HL60 human myeloid leukemia cells to biological regulators. , 1983, Leukemia research.

[15]  H P Koeffler,et al.  Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. , 1983, Blood.

[16]  F. Ruscetti,et al.  Induction of differentiation of HL-60 cells by dimethyl sulfoxide: evidence for a stochastic model not linked to the cell division cycle. , 1982, Cancer research.

[17]  M. Andreeff,et al.  Detection of luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60. , 1981, Experimental hematology.

[18]  C. Abboud,et al.  Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line, GCT. , 1981, Blood.

[19]  R. Schooley,et al.  Elevated serum levels of the eosinophil granule major basic protein in patients with eosinophilia. , 1981, The Journal of clinical investigation.

[20]  M. Andreeff,et al.  Changes of cellular markers during differentiation of HL-60 promyelocytes to macrophages as induced by T lymphocyte conditioned medium. , 1981, Leukemia research.

[21]  H. Koeffler,et al.  Action of granulocyte-macrophage colony-stimulating factors: studies using a human leukemia cell line. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Collins,et al.  Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[23]  P. Glaser,et al.  A new approach to the morphological and cytochemical evaluation of human bone marrow CFUC in agar cultures. , 1980, Experimental hematology.

[24]  N. Olashaw,et al.  Terminal differentiation in human promyelocytic leukaemic cells in the absence of DNA synthesis , 1980, Nature.

[25]  D. Bass,et al.  Differential regulation of spleen cell-mediated eosinophil and neutrophil-macrophage production. , 1980, Blood.

[26]  D. Loegering,et al.  Cytotoxic properties of the eosinophil major basic protein. , 1979, Journal of immunology.

[27]  S. Collins,et al.  Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. , 1979, Blood.

[28]  S. Collins,et al.  Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[29]  S. Collins,et al.  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture , 1977, Nature.

[30]  G. Johnson,et al.  Heterogeneity in human neutrophil, macrophage and eosinophil progenitor cells demonstrated by velocity sedimentation separation. , 1977, Blood.

[31]  D. Metcalf,et al.  Eosinophil and neutrophil colony-forming cells in culture. , 1977, Blood.

[32]  G. Johnson,et al.  Eosinophil colony formation in semisolid cultures of human bone marrow cells. , 1977, Blood.

[33]  G. Grusky,et al.  The identification of eosinophil colonies in soft-agar cultures by differential staining for peroxidase. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[34]  F. Ruscetti,et al.  Specific release of neutrophillic- and eosinophilic-stimulating factors from sensitized lymphocytes. , 1976, Blood.

[35]  L. Rozenszajn,et al.  Cytochemical and morphologic identification of macrophages and eosinophils in tissue cultures of normal human bone marrow. , 1974, Blood.

[36]  C. Li,et al.  ESTERASES IN HUMAN LEUKOCYTES , 1973, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[37]  W. Crosby,et al.  Cytochemical identification of monocytes and granulocytes. , 1971, American journal of clinical pathology.

[38]  H. Koeffler,et al.  y-Interferon induces expression of the HLA-D antigens on normal and leukemic human myeloid cells ( major histocompatibility complex class II antigens ) , 2022 .