α-Actinin: Immunofluorescent localization of a muscle structural protein in nonmuscle cells

Antibodies specific for the skeletal muscle structural protein alpha-actinin are used to localize this protein by indirect immunofluorescence in nonmuscle cells. In cultured nonmuscle cells, alpha-actinin is localized along or between actin filament bundles producing an almost regular periodicity. The protein is also detected in the form of fluorescent plaques at some ends of actin filament bundles, as well as in a filamentous form in some overlap areas of cells. In spreading rat embryo cells, alpha-actinin assumes a focal distribution which corresponds to the vertices of a highly regular actin filament network. The results suggest that alpha-actinin may be involved in the organization of actin filament bundles, in the attachment of actin filaments to the plasma membrane, and in the assembly of actin filaments in areas of cell to cell contact.

[1]  H. Ishikawa,et al.  FORMATION OF ARROWHEAD COMPLEXES WITH HEAVY MEROMYOSIN IN A VARIETY OF CELL TYPES , 1969, The Journal of cell biology.

[2]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[3]  M. Abercrombie,et al.  The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. , 1971, Experimental cell research.

[4]  K. Burridge,et al.  Association of actin and myosin with secretory granule membranes , 1975, Nature.

[5]  D. E. Goll,et al.  Studies on purified -actinin. I. Effect of temperature and tropomyosin on the -actinin-F-actin interaction. , 1972, Journal of molecular biology.

[6]  E. Lazarides.  Immunofluorescence studies on the structure of actin filaments in tissue culture cells. , 1975, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[7]  E. Lazarides,et al.  The distribution of actin in non-muscle cells. The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells. , 1975, Experimental cell research.

[8]  K. Porter,et al.  An electron microscope study of differentiation of the molting muscles of Rhodnius prolixus. , 1969, The American journal of anatomy.

[9]  A. Rich,et al.  Actin associated with membranes from 3T3 mouse fibroblast and HeLa cells , 1975, The Journal of cell biology.

[10]  D. E. Goll,et al.  Studies on purified -actinin. II. Electron microscopic studies on the competitive binding of -actinin and tropomyosin to Z-line extracted myofibrils. , 1972, Journal of molecular biology.

[11]  J. Spudich.  Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. II. Purification, properties, and membrane association of actin from amoebae of Dictyostelium discoideum. , 1974, The Journal of biological chemistry.

[12]  J. E. Heaysman,et al.  Early contacts between fibroblasts. An ultrastructural study. , 1973, Experimental cell research.

[13]  D. Spiro,et al.  DERIVATION OF THE Z LINE IN THE EMBRYONIC CHICK HEART , 1970, The Journal of cell biology.

[14]  T. Pollard,et al.  Electron microscopic identification of actin associated with isolated amoeba plasma membranes. , 1973, The Journal of biological chemistry.

[15]  E. Lazarides.  Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells , 1975, The Journal of cell biology.

[16]  L. Tskhovrebova,et al.  Electron microscopic study of ?-actinin , 1975 .

[17]  RL Murray,et al.  The occurrence of actinlike filaments in association with migrating pigment granules in frog retinal pigment epithelium , 1975, The Journal of cell biology.