Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer

Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so far only in weakly scattering samples. We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope. In the highly scattering rat brain tissue, where multiply scattered photons falling within the temporal gate of the CGWS can no longer be neglected, we have measured known defocus and spherical aberrations up to a depth of 400 µm.

[1]  M. Gustafsson,et al.  Phase‐retrieved pupil functions in wide‐field fluorescence microscopy , 2004, Journal of microscopy.

[2]  T. Wilson,et al.  Aberration correction for confocal imaging in refractive‐index‐mismatched media , 1998 .

[3]  Peter T. C. So,et al.  Self-referenced quantitative phase microscopy , 2012, Photonics West - Biomedical Optics.

[4]  J. Girkin,et al.  Practical implementation of adaptive optics in multiphoton microscopy. , 2003, Optics express.

[5]  Winfried Denk,et al.  Coherence-gated wave-front sensing in strongly scattering samples. , 2004, Optics letters.

[6]  A V Larichev,et al.  Measurement of eye aberrations in a speckle Øeld , 2001 .

[7]  S. Gigan,et al.  Brain refractive index measured in vivo with high-NA defocus-corrected full-field OCT and consequences for two-photon microscopy. , 2011, Optics express.

[8]  Tony Wilson,et al.  Image-based adaptive optics for two-photon microscopy. , 2009, Optics letters.

[9]  R. Noll.  Zernike polynomials and atmospheric turbulence , 1976 .

[10]  D. Kobat,et al.  In vivo two-photon microscopy to 1.6-mm depth in mouse cortex. , 2011, Journal of biomedical optics.

[11]  S. Gigan,et al.  Measuring known aberrations in rat brain slices with Coherence-Gated Wavefront Sensor based on a Linnik interferometer , 2012 .

[12]  T. Wilson,et al.  Adaptive aberration correction in a confocal microscope , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Mertz.  Introduction to Optical Microscopy , 2009 .

[14]  Eric Betzig,et al.  Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues , 2010, Nature Methods.

[15]  Simon Tuohy,et al.  Depth-resolved wavefront aberrations using a coherence-gated Shack-Hartmann wavefront sensor. , 2010, Optics express.

[16]  T. Wilson,et al.  An optical technique for remote focusing in microscopy , 2008 .

[17]  Daniel Malacara,et al.  Phase shifting interferometry * , 2008 .

[18]  Takashi R Sato,et al.  Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex , 2011, Proceedings of the National Academy of Sciences.

[19]  F. He,et al.  Extension of imaging depth in two‐photon fluorescence microscopy using a long‐wavelength high‐pulse‐energy femtosecond laser source , 2011, Journal of microscopy.

[20]  Rudiger Gens,et al.  Two-dimensional phase unwrapping for radar interferometry: Developments and new challenges , 2003 .

[21]  J. Fujimoto,et al.  Determination of the refractive index of highly scattering human tissue by optical coherence tomography. , 1995, Optics letters.

[22]  G. Mourou,et al.  Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy. , 2000, Optics letters.

[23]  Rimas Juškaitis,et al.  Characterizing High Numerical Aperture Microscope Objective Lenses , 2003 .

[24]  J. Goodman.  Some fundamental properties of speckle , 1976 .

[25]  Winfried Denk,et al.  Properties of coherence-gated wavefront sensing. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[26]  Zahid Yaqoob,et al.  A reflection-mode configuration for enhanced light delivery through turbidity , 2012, Photonics West - Biomedical Optics.

[27]  R. Crane.  Interference phase measurement , 1991 .

[28]  T. Wilson,et al.  Adaptive optics for structured illumination microscopy. , 2008, Optics express.

[29]  W. Denk,et al.  Deep tissue two-photon microscopy , 2005, Nature Methods.

[30]  Jerome Mertz,et al.  Two-photon microscopy in brain tissue: parameters influencing the imaging depth , 2001, Journal of Neuroscience Methods.

[31]  Sylvain Gigan,et al.  Measuring aberrations in the rat brain by a new coherence-gated wavefront sensor using a Linnik interferometer , 2012, Photonics West - Biomedical Optics.

[32]  Tony Wilson,et al.  New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[33]  Andrey V. Larichev,et al.  ERRATA: Measurement of eye aberrations in a speckle field , 2001 .

[34]  J Cariou,et al.  Scattering through fluids: speckle size measurement and Monte Carlo simulations close to and into the multiple scattering. , 2004, Optics express.

[35]  Louis A. Romero,et al.  A Cellular Automata Method for Phase Unwrapping , 1986, Topical Meeting On Signal Recovery and Synthesis II.

[36]  D. Kleinfeld,et al.  Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[37]  W. Denk,et al.  Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing , 2006, Proceedings of the National Academy of Sciences.