A novel acetylcholinesterase biosensor based on ionic liquids-AuNPs-porous carbon composite matrix for detection of organophosphate pesticides

Abstract A novel acetylcholinesterase (AChE) biosensor, based on honeycomb-like hierarchically ion liquids ([BSmim]HSO 4 )-AuNPs-porous carbon composite modified boron-doped diamond (BDD) electrode, was developed for the detection of organophosphate pesticides. The surface morphology of the prepared [BSmim]HSO 4 -AuNPs-porous carbon composite was characterized by scanning electron microscopy and transmission electron microscopy. The [BSmim]HSO 4 -AuNPs-porous carbon modified BDD electrode was confirmed by cyclic voltammogram and electrochemical impedance spectroscopy. For the oxidation of thiocholine, hydrolysis product of acetylthiocholine, the peak current at AChE/[BSmim]HSO 4 -AuNPs-porous carbon/BDD electrode is more than 4.5 times that at AChE/BDD electrode. The inhibition of dichlorvos is linearly proportional to its concentration in the range of 10 −10 –10 −6  g/L (4.5 × 10 −13 –4.5 × 10 −9  M), with the detection limit of 6.61 × 10 −11  g/L (2.99 × 10 −13  M) (calculated for 10% inhibition). The proposed biosensor provided an efficient and promising platform for the immobilization of AChE and exhibited higher sensitivity and acceptable stability for the detection of organophosphate pesticides.

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