Absorption, fluorescence, and quantum yield for growth in nitrogen-limited Dunaliella tertiolecta

The effects of steady state nitrogen limitation on the optical properties of Dunalielh tertiolecta were investigated. Growth rate was varied in a continuous culture under constant irradiance and temperature with absorption, fluorescence, and cellular characteristics including pigment contents determined at each steady state. The cellular concentration of Chl a increased with growth rate while Chl-a-specific absorption and fluorescence both decreased. In addition, the quantum yield for growth varied by more than a factor of 3.5 over the growth conditions examined, with the highest yield in the most rapidly growing cells. The decrease in magnitude of Chl-a-specific optical properties is caused by pigment package effects and changes in the abundance of accessory pigments relative to Chl a. Changes in absorption and fluorescence properties are consistent with theoretical predictions for discrete packages. In addition, pigment-based reconstruction techniques overestimate the magnitude ofboth in vivo and unpackaged absorption by D. tertiolecta under these growth conditions. The observed variability in Chl-a-specific absorption and quantum yield is a fundamental aspect of phytoplankton physiology that should be incorporated into models of oceanic primary production.

[1]  L. Duysens,et al.  The flattening of the absorption spectrum of suspensions, as compared to that of solutions. , 1956, Biochimica et biophysica acta.

[2]  Annick Bricaud,et al.  Optical efficiency factors of some phytoplankters1 , 1983 .

[3]  D. Kiefer,et al.  Chlorophyll a fluorescence in marine centric diatoms: Responses of chloroplasts to light and nutrient stress , 1973 .

[4]  R. Bidigare.  Photosynthetic Pigment Composition of the Brown Tide Alga: Unique Chlorophyll and Carotenoid Derivatives , 1989 .

[5]  D. Kiefer,et al.  A steady state description ofgrowth and light absorption in the marine planktonic diatom Skeletonema costatum , 1989 .

[6]  P. Falkowski,et al.  NITROGEN LIMITATION IN ISOCHRYSIS GALBANA (HAPTOPHYCEAE). I. PHOTOSYNTHETIC ENERGY CONVERSION AND GROWTH EFFICIENCIES 1 , 1989 .

[7]  T. T. Bannister.  Quantitative description of steady state, nutrient‐saturated algal growth, including adaptation , 1979 .

[8]  Judith Meyer,et al.  Nitrogen-limited growth of marine phytoplankton—II. Uptake kinetics and their role in nutrient limited growth of phytoplankton , 1972 .

[9]  Dale A. Kiefer,et al.  A simple, steady state description of phytoplankton growth based on absorption cross section and quantum efficiency1 , 1983 .

[10]  E. Laws,et al.  A test of the assumptions and predictions of recent microalgal growth models with the marine phytoplankter Pavlova lutheri , 1990 .

[11]  A. Bricaud,et al.  Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton , 1981 .

[12]  Karen S. Baker,et al.  Oceanic primary production estimates from measurements of spectral irradiance and pigment concentrations , 1987 .

[13]  D. Collins,et al.  The role of reabsorption in the spectral distribution of phytoplankton fluorescence emission , 1985 .

[14]  Shubha Sathyendranath,et al.  Variations in the spectral values of specific absorption of phytoplankton , 1987 .

[15]  M. Perry,et al.  Quantum yield, relative specific absorption and fluorescence in nitrogen-limited Chaetoceros gracilis , 1987 .

[16]  Karen S. Baker,et al.  Bio‐optical modeling of photosynthetic production in coastal waters , 1989 .

[17]  A. Morel.  Optical modeling of the upper ocean in relation to its biogenous matter content (case I waters) , 1988 .

[18]  N. Welschmeyer,et al.  CHLOROPHYLL‐SPECIFIC PHOTOSYNTHESIS AND QUANTUM EFFICIENCY AT SUBSATURATING LIGHT INTENSITIES 1 2 , 1981 .

[19]  P. Falkowski,et al.  Effects of Growth Irradiance and Nitrogen Limitation on Photosynthetic Energy Conversion in Photosystem II. , 1988, Plant physiology.

[20]  Annick Bricaud,et al.  Optical properties of diverse phytoplanktonic species: experimental results and theoretical interpretation , 1988 .

[21]  A. R. Loeblich.  A SEAWATER MEDIUM FOR DINOFLAGELLATES AND THE NUTRITION OF CACHONINA NIEI1 , 1975 .

[22]  Judith Meyer,et al.  Nitrogen-limited growth of marine phytoplankton—I. changes in population characteristics with steady-state growth rate , 1972 .

[23]  R. Olson,et al.  Chlorophyll fluorescence from single cells: Interpretation of flow cytometric signals , 1989 .

[24]  Paul G. Falkowski,et al.  Growth‐irradiance relationships in phytoplankton1 , 1985 .

[25]  Richard W. Spinrad,et al.  A Low Power, High Resolution, In Situ Fluorometer For Profiling And Moored Applications In Water , 1988, Defense, Security, and Sensing.

[26]  B. Prézelin,et al.  Chromatic light effects and physiological modeling of absorption properties of Heterocapsa pygmaea (=Glenodinium sp.). , 1990 .

[27]  P. Falkowski,et al.  PHOTOADAPTATION AND THE “PACKAGE” EFFECT IN DUNALIELLA TERTIOLECTA (CHLOROPHYCEAE) 1 , 1989 .