Immobilization of poly(ethylene imine) onto polymer films pretreated with plasma

Poly(ethylene imine) (PEI)-immobilized surfaces have attracted much attention as interesting biomaterials in relation to cell culture, selective adsorption of endotoxins, and so on. In this study, we found that PEI could be effectively immobilized on various polymers pretreated by oxygen plasma. Surface analyses using an X-ray photoelectron spectrometer and contents of peroxide groups determined by iodide method indicate that the hydroxy peroxides were the main functional groups that contributed to PEI immobilization covalently on the polymer surfaces treated with oxygen plasma. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 75: 576–582, 2000

[1]  Y Ikada,et al.  Immobilization of DNA onto a polymer support and its potentiality as immunoadsorbent , 2000, Biotechnology and bioengineering.

[2]  M. Vijayalakshmi,et al.  Endotoxin removal with poly(ethyleneimine)-immobilized adsorbers: Sepharose 4B versus flat sheet and hollow fibre membranes. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[3]  R. Caudano,et al.  Amination of Poly(ethylene-terephthalate) polymer surface for biochemical applications , 1996 .

[4]  P. Favia,et al.  Selective grafting of amine groups on polyethylene by means of NH3−H2 RF glow discharges , 1996 .

[5]  D. Falkenhagen,et al.  Development of cationically modified cellulose adsorbents for the removal of endotoxins. , 1995, ASAIO journal.

[6]  Y. Kim,et al.  In vitro studies of immobilized heparin and sulfonated polyurethane using epifluorescent video microscopy. , 1995, ASAIO journal (1992).

[7]  Y. Ikada,et al.  Peroxide generation and decomposition on polymer surface , 1995 .

[8]  M. Matsumura,et al.  High density cultivation of hybridoma in charged porous carriers. , 1994, Journal of biotechnology.

[9]  C. J. Pike,et al.  Surface determinants of neuronal survival and growth on self-assembled monolayers in culture , 1993, Brain Research.

[10]  G. Rebel,et al.  Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates , 1992, Journal of neuroscience research.

[11]  Y. Ikada,et al.  GRAFT COPOLYMERIZATION OF ACRYLAMIDE ONTO A POLYETHYLENE SURFACE PRETREATED WITH GLOW DISCHARGE , 1986 .

[12]  R. Nuzzo,et al.  Preparation and characterization of functionalized polyethylene surfaces , 1984 .

[13]  G. Banker,et al.  Culturing nerve cells , 1998 .

[14]  J. Feijen,et al.  Introduction of amine groups on poly(ethylene) by plasma immobilization of a preadsorbed layer of decylamine hydrochloride. , 1993, Journal of biomaterials science. Polymer edition.

[15]  Y. Yao,et al.  Surface modification of high-density polyethylene by plasma treatment , 1993 .

[16]  A S Hoffman,et al.  Immobilization of surface active compounds on polymer supports using a gas discharge process. , 1992, Journal of biomaterials science. Polymer edition.

[17]  H. Mark,et al.  Encyclopedia of polymer science and engineering , 1985 .

[18]  C. Finch.  Chemistry and Technology of Water-Soluble Polymers , 1983 .