In vitro free radical scavenging activity of Sorghum bicolor ( L . )

Sorghum (Sorghum bicolour (L) Monech) is the fifth leading cereal crop in the world and is used primarily in Asia and Africa as food crop. Sorghum is an economically important food crop, it is also used for treatment of several ailments like cardiovascular health, reduce the risk of certain types of cancer and reduce obesity in humans. In the present study, the antioxidant properties of Acetone-water extract from eight genotypes of sorghum were estimated by three different methods. The percentage inhibition of superoxide, hydroxyl and lipid peroxidases were maximum at 100, 300, 1200 μg respectively. The percentage of inhibitions increased with increasing concentrations of extract. Of the eight genotypes, IS-2695 shown maximum of 64.99% inhibition for superoxide radical and the genotype IS-7155 was shown maximum inhibition in hydroxyl, lipid peroxides 67.92% and 82.06% in this order. Significant differences were obtained among the genotypes when the antioxidant activity of the each genotype subjected to a two-way ANOVA.

[1]  R. Willson.  Iron, zinc, free radicals and oxygen in tissue disorders and cancer control. , 2008, Ciba Foundation symposium.

[2]  L. Rooney,et al.  Phenolic compounds and antioxidant activity of sorghum grains of varying genotypes. , 2005, Journal of agricultural and food chemistry.

[3]  J. Awika,et al.  Sorghum phytochemicals and their potential impact on human health. , 2004, Phytochemistry.

[4]  Luis Cisneros-Zevallos,et al.  Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products. , 2003, Journal of agricultural and food chemistry.

[5]  M. Manley,et al.  Antioxidant activity of South African red and white cultivar wines: free radical scavenging. , 2003, Journal of agricultural and food chemistry.

[6]  G. Beecher,et al.  Fractionation of polymeric procyanidins from lowbush blueberry and quantification of procyanidins in selected foods with an optimized normal-phase HPLC-MS fluorescent detection method. , 2002, Journal of agricultural and food chemistry.

[7]  C. Rice-Evans,et al.  The Antioxidant Activity of Regularly Consumed Fruit and Vegetables Reflects their Phenolic and Vitamin C Composition , 2002, Free radical research.

[8]  K. Stettmaier,et al.  Electron paramagnetic resonance studies of radical species of proanthocyanidins and gallate esters. , 2000, Archives of biochemistry and biophysics.

[9]  G. Jones,et al.  High Molecular Weight Plant Polyphenolics (Tannins) as Biological Antioxidants. , 1998, Journal of agricultural and food chemistry.

[10]  P. Pietta,et al.  Polyphenol Content and Total Antioxidant Potential of Selected Italian Wines , 1997 .

[11]  A. Waterhouse,et al.  Principal Phenolic Phytochemicals in Selected California Wines and Their Antioxidant Activity in Inhibiting Oxidation of Human Low-Density Lipoproteins , 1995 .

[12]  R. Waniska,et al.  Effects of growth conditions on grain molding and phenols in sorghum caryopsis , 1989 .

[13]  R. Bandyopadhyay,et al.  Polyphenol concentrations in grain, leaf, and callus tissues of mold-susceptible and mold-resistant sorghum cultivars , 1986 .

[14]  I. Fridovich,et al.  Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). , 1969, The Journal of biological chemistry.

[15]  B. Halliwell,et al.  The importance of free radicals and catalytic metal ions in human diseases. , 1985, Molecular aspects of medicine.

[16]  L. Rooney,et al.  Tannins and phenols of sorghum , 1984 .

[17]  L. Rooney,et al.  Sorghum phenolic acids, their high performance liquid chromatography separation and their relation to fungal resistance , 1983 .

[18]  大川 博,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction , 1979 .