Quantitative phase microscopy of transparent samples using a liquid crystal display

Abstract. We propose a new quantitative phase microscope based on spatial filtering of the beam carrying the sample-induced wavefront. A prototype built using a transmissive liquid crystal display for the experimental demonstration of the principle and preliminary results obtained with artificial and biological samples are presented.

[1]  Alexander Jesacher,et al.  Quantitative imaging of complex samples by spiral phase contrast microscopy. , 2006, Optics express.

[2]  Tim N. Ford,et al.  Quantitative phase imaging using a partitioned detection aperture. , 2012, Optics letters.

[3]  Rama Chellappa,et al.  A Method for Enforcing Integrability in Shape from Shading Algorithms , 1988, IEEE Trans. Pattern Anal. Mach. Intell..

[4]  B. Wattellier,et al.  Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells. , 2009, Optics express.

[5]  E. Cuche,et al.  Cell refractive index tomography by digital holographic microscopy. , 2006, Optics letters.

[6]  Gabriel Popescu,et al.  Gradient field microscopy of unstained specimens , 2012, Optics express.

[7]  Zahid Yaqoob,et al.  Quantitative DIC microscopy using an off-axis self-interference approach. , 2010, Optics letters.

[8]  Roberto Ragazzoni,et al.  Extended source pyramid wave-front sensor for the human eye. , 2002, Optics express.

[9]  Christophe Verinaud,et al.  On the nature of the measurements provided by a pyramid wave-front sensor , 2004 .

[10]  L. García-Marín,et al.  Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen. , 2009, Theriogenology.

[11]  R. Ragazzoni.  Pupil plane wavefront sensing with an oscillating prism , 1996 .

[12]  G. Saavedra,et al.  Chapter 1 The Resolution Challenge in 3D Optical Microscopy , 2009 .

[13]  M R Arnison,et al.  Linear phase imaging using differential interference contrast microscopy , 2004, Journal of microscopy.

[14]  Gabriel Popescu,et al.  Laplace field microscopy for label-free imaging of dynamic biological structures. , 2011, Optics letters.

[15]  G. Barbastathis,et al.  Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. , 2010, Optics letters.

[16]  E. H. Linfoot.  On the interpretation of the Foucault test , 1948, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[17]  I. Iglesias.  Pyramid phase microscopy. , 2011, Optics letters.

[18]  D Rath,et al.  Differing sperm ability to penetrate the oocyte in vivo and in vitro as revealed using colloidal preparations. , 2009, Theriogenology.

[19]  P Memmolo,et al.  Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy. , 2011, Optics express.