Proteins of rat serum IV. Time‐course of acute‐phase protein expression and its modulation by indomethacine

Changes in the concentration of major serum proteins were monitored from day 0 to day 4 in three experimental groups: rats injected with turpentine, rats receiving the turpentine shot and daily doses of indomethacine, and rats given indomethacine alone. In inflamed animals, peak changes for acute‐phase reactants, evaluated by two‐dimensional electrophoresis (2‐DE), were usually observed between 48 and 72 h after the phlogistic stimulus. By itself, indomethacine was found to affect the synthesis of most proteins (except one of the thiostatin variants and ceruloplasmin); the changes in serum levels, whether positive or negative, were the same as upon inflammation (except for kallikrein‐binding protein), but their extent and/or timing usually differed. When inflamed animals were given indomethacine, a clear‐cut difference in the concentration of some proteins was observed versus inflamed rats not given medication, at 24 h after the start of the treatments. Proteins mainly affected were α2‐macroglobulin, α2‐HS‐glycoprotein, C‐reactive protein and kallikrein‐binding protein.