Piezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film.

A polymer sorbent formed at the surface of gold-plated piezoelectric crystal by anodic oxidation of m-aminophenol was investigated by voltammetric, FTIR techniques for antibody coupling after activation by divinylsulphone. A novel film with increased capacity for immobilizing antibodies was obtained using phloroglucinol to modify poly-m-aminophenol via divinylsulphone. Compared with the dip-coating methods using polyethylenimine and (gamma-aminopropyl)trimethoxysilane, this new technique gave more reproducible results for the immobilization of antibody from sample to sample due to the improved homogeneity and reproducibility of the coating. With present modification method, a piezoelectric immunosensor was developed for the detection of Salmonella enteritidis. A detection limit of 1x10(5) cells ml(-1) and an assay time of 25 min were achieved.

[1]  S. Cattarin,et al.  Electrosynthesis and properties of ring-substituted polyanilines☆ , 1988 .

[2]  W. C. Purdy,et al.  Evaluation of amine-functionalized coatings for liquid-phase QCM applications , 1998 .

[3]  F. Caruso,et al.  Quartz crystal microbalance study of DNA immobilization and hybridization for nucleic Acid sensor development. , 1997, Analytical chemistry.

[4]  Maria Minunni,et al.  The Quartz Crystal Microbalance as Biosensor. A Status Report on Its Future , 1995 .

[5]  I. Willner,et al.  Piezoelectric immunosensors for urine specimens of Chlamydia trachomatis employing quartz crystal microbalance microgravimetric analyses. , 1997, Analytical chemistry.

[6]  D. Buttry,et al.  Determination of ion populations and solvent content as functions of redox state and pH in polyaniline , 1987 .

[7]  M. Grätzel,et al.  A novel immunosensor for herpes viruses. , 1994, Analytical chemistry.

[8]  T. Nomura,et al.  Determination of lead in solution with a piezoelectric quartz crystal coated with copper oleate , 1986 .

[9]  Joseph Wang,et al.  Dendritic Nucleic Acid Probes for DNA Biosensors , 1998 .

[10]  L. Tessier,et al.  Potential of the thickness shear mode acoustic immunosensors for biological analysis , 1997 .

[11]  A. Gehring,et al.  Immunoelectrochemical assays for bacteria: use of epifluorescence microscopy and rapid-scan electrochemical techniques in development of an assay for Salmonella. , 1996, Analytical chemistry.

[12]  Mary Lou Tortorello,et al.  Fluorescent Antibodies Applied to Direct Epifluorescent Filter Technique for Microscopic Enumeration of Escherichia coli 0157:H7 in Milk and Juice. , 1993, Journal of food protection.

[13]  P. Lacaze,et al.  Obtaining thin films of “reactive polymers” on metal surfaces by electrochemical polymerization part I. Reactivity of functional groups in a carbonyl substituted polyphenylene oxide film , 1978 .

[14]  J. Porath,et al.  High capacity chemisorbents for protein immobilization. , 1972, Nature: New biology.

[15]  Asim K. Bej,et al.  Detection of Salmonella spp. in Oysters Using Polymerase Chain Reactions (PCR) and Gene Probes , 1993 .

[16]  I Karube,et al.  A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors. , 1996, Analytical chemistry.

[17]  J H Luong,et al.  Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium. , 1990, Enzyme and microbial technology.

[18]  G. Wallace,et al.  Pulsed electrochemical detection of proteins using conducting polymer based sensors , 1995 .