Effect of effective microorganisms on wheat growth under salt stress condition

Effective microorganisms (EM) are important beneficial fungi and bacteria mixed culture that is used as an inoculant to improve soil quality and health, enhance plant growth, yield, and quality. This study was conducted to evaluate the effect of inoculating various concentration of EM (50, 100 and 200 mL L -1 ) on growth of wheat ( Triticum aestivum L.) seedlings, grown under NaCl regime (6 dS m -1 ), including fresh and dry weight, chlorophyll content, maximum fluorescence yield (F m ), variable fluorescence yield (F v ), optimum photosystem II (PSII) quantum yield (QY) and ratio of fluorescence decline in steady state (RFD). Seedlings subjected to salt treatment exhibited significantly 60% and 30% reduction in both fresh in dry weight; respectively, however, when seedlings under saline condition were treated with EM at both 50 and 100 mL -1 , fresh weight was increased two folds. However, for both dry weight and plant length, EM at 100 mL -1 showed the highest significant values compared to salt treated seedlings (40% and 18%, respectively). Salinity treatment reduced significantly chlorophyll content, F v /F m and QY, however, the addition of EM at 200 mL L -1 alleviated the effect of salt stress on photosynthetic parameters. The application of both 100 and 200 mL L -1 alleviated the salt effect resulted in a similar chlorophylls content as the control (36.23 and 34.12 SPAD units) respectively. EM application at 200 mL L -1 significantly lowered electrical conductivity from 14.35 to 10.29 dS m -1 of the media. In conclusion, EM can be used to counter act salt effect in soil and improve growth behavior of wheat plants under salinity conditions. (RFD) Emission taken by (CCD) in FS-FI-2200 FluorCam end when and the including and then at for Plant also for each soil samples taken from each and analyzed for chemical properties. Data analyzed using Minitab 18 software (Minitab, ANOVA was conducted followed by mean separation using LSD test at 0.05 probability level, numbers were presented as averages.

[1]  G. Chao,et al.  Effects of effective microorganisms biochar-based fertilizer on photosynthetic characteristics and chlorophyll content of flue-cured tobacco under water-saving irrigation strategies , 2020 .

[2]  Ivan Simko,et al.  Phenomic and Physiological Analysis of Salinity Effects on Lettuce , 2019, Sensors.

[3]  She Dongli,et al.  Effect of effective microorganism and gypsum amendments on nutrient leaching, pH, electrical conductivity, and Okra growth parameters under coastal saline soil , 2018, Communications in Soil Science and Plant Analysis.

[4]  M. Iqbal,et al.  Reducing water salinity using effective microorganisms , 2017 .

[5]  H. Q. Li,et al.  Inoculation with plant growth-promoting bacteria (PGPB) improves salt tolerance of maize seedling , 2017, Russian Journal of Plant Physiology.

[6]  H. Shao,et al.  Biochar applied with appropriate rates can reduce N leaching, keep N retention and not increase NH3 volatilization in a coastal saline soil. , 2017, The Science of the total environment.

[7]  S. Wani,et al.  Rhizobacterial-plant interactions: Strategies ensuring plant growth promotion under drought and salinity stress , 2016 .

[8]  P. Jha,et al.  The Multifarious PGPR Serratia marcescens CDP-13 Augments Induced Systemic Resistance and Enhanced Salinity Tolerance of Wheat (Triticum aestivum L.) , 2016, PloS one.

[9]  A. Oukarroum,et al.  Effective microorganisms impact on photosynthetic activity of Arabidopsis plant grown under salinity stress conditions , 2016 .

[10]  Hongyuan Wang,et al.  Effect of biochar additions to soil on nitrogen leaching, microbial biomass and bacterial community structure , 2016 .

[11]  H. Alikhani,et al.  Promotion of Wheat Growth under Salt Stress by Halotolerant Bacteria Containing ACC deaminase , 2016 .

[12]  F. Bayat,et al.  Screening fluorescent pseudomonads isolated from wheat rhizosphere for plant growth-promoting and salt tolerance properties , 2016 .

[13]  N. B. Talaat.  Effective microorganisms modify protein and polyamine pools in common bean (Phaseolus vulgaris L.) plants grown under saline conditions , 2015 .

[14]  W. Davies,et al.  Rhizobacteria that produce auxins and contain 1-amino-cyclopropane-1-carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well-watered and water-limited potato (Solanum tuberosum) , 2015 .

[15]  M. Pontin,et al.  Azospirillum brasilense ameliorates the response of Arabidopsis thaliana to drought mainly via enhancement of ABA levels. , 2015, Physiologia plantarum.

[16]  D. Singh,et al.  Effect of salt-tolerant plant growth-promoting rhizobacteria on wheat plants and soil health in a saline environment. , 2015, Plant biology.

[17]  Z. Rengel,et al.  Applying geostatistics to determine the soil quality improvement by Jerusalem artichoke in coastal saline zone , 2014 .

[18]  J. Schroeder,et al.  Plant salt-tolerance mechanisms. , 2014, Trends in plant science.

[19]  E. Nevo,et al.  Drought-Tolerance of Wheat Improved by Rhizosphere Bacteria from Harsh Environments: Enhanced Biomass Production and Reduced Emissions of Stress Volatiles , 2014, PloS one.

[20]  Byung-Taek Oh,et al.  Alleviation of Salt Stress by Enterobacter sp. EJ01 in Tomato and Arabidopsis Is Accompanied by Up-Regulation of Conserved Salinity Responsive Factors in Plants , 2014, Molecules and cells.

[21]  R. Azcón,et al.  Differential Activity of Autochthonous Bacteria in Controlling Drought Stress in Native Lavandula and Salvia Plants Species Under Drought Conditions in Natural Arid Soil , 2013, Microbial Ecology.

[22]  Elisabeth N. Bui,et al.  Soil salinity: A neglected factor in plant ecology and biogeography , 2013 .

[23]  Cheng Hu,et al.  Long-term effective microorganisms application promote growth and increase yields and nutrition of wheat in China , 2013 .

[24]  S. Arabia,et al.  ASSESSMENT OF SALT TOLERANCE OF SOME NEWLY DEVELOPED AND CANDIDATE WHEAT (TRITICUM AESTIVUM L.) CULTIVARS USING GAS EXCHANGE AND CHLOROPHYLL FLUORESCENCE ATTRIBUTES , 2011 .

[25]  M. Abdeshahian,et al.  Chlorophyll Fluorescence as Criterion for the Diagnosis Salt Stress in Wheat (Triticum aestivum) Plants , 2010 .

[26]  S. Hasnain,et al.  Comparative study of wild and transformed salt tolerant bacterial strains on Triticum aestivum growth under salt stress , 2010, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[27]  Heikham Evelin,et al.  Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. , 2009, Annals of botany.

[28]  D. Egamberdieva,et al.  Selection for root colonising bacteria stimulating wheat growth in saline soils , 2009, Biology and Fertility of Soils.

[29]  Conceição Santos.  Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves , 2004 .

[30]  T. Mahmood,et al.  Inoculating wheat seedlings with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress , 2004, Biology and Fertility of Soils.

[31]  H. Bohnert,et al.  PLANT CELLULAR AND MOLECULAR RESPONSES TO HIGH SALINITY. , 2000, Annual review of plant physiology and plant molecular biology.

[32]  K Maxwell,et al.  Chlorophyll fluorescence--a practical guide. , 2000, Journal of experimental botany.