Selection and Biosensor Application of Aptamers for Small Molecules

Small molecules play a major role in the human body and as drugs, toxins, and chemicals. Tools to detect and quantify them are therefore in high demand. This review will give an overview about aptamers interacting with small molecules and their selection. We discuss the current state of the field, including advantages as well as problems associated with their use and possible solutions to tackle these. We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water contaminations to RNA imaging.

[1]  M. Stojanović,et al.  Modular aptameric sensors. , 2004, Journal of the American Chemical Society.

[2]  A. Roche,et al.  How effective is drug testing as a workplace safety strategy? A systematic review of the evidence. , 2014, Accident; analysis and prevention.

[3]  Piero Migliorato,et al.  Electrochemical aptamer-based sandwich assays for the detection of explosives. , 2012, Analytical chemistry.

[4]  Ying Wang,et al.  In situ live cell sensing of multiple nucleotides exploiting DNA/RNA aptamers and graphene oxide nanosheets. , 2013, Analytical chemistry.

[5]  Weisheng Liu,et al.  Determination of free tryptophan in serum with aptamer--comparison of two aptasensors. , 2015, Talanta.

[6]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[7]  J. Szostak,et al.  Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures , 1992, Nature.

[8]  Chunhai Fan,et al.  DNA nanostructure-decorated surfaces for enhanced aptamer-target binding and electrochemical cocaine sensors. , 2011, Analytical chemistry.

[9]  J. Feigon,et al.  Solution structure of an ATP-binding RNA aptamer reveals a novel fold. , 1997, RNA.

[10]  Shin-ichi Sato,et al.  Live-cell imaging of endogenous mRNAs with a small molecule. , 2015, Angewandte Chemie.

[11]  Bulent Ozpolat,et al.  Preclinical and clinical development of siRNA-based therapeutics. , 2015, Advanced drug delivery reviews.

[12]  Kun Wang,et al.  Label-free impedimetric aptasensor for detection of femtomole level acetamiprid using gold nanoparticles decorated multiwalled carbon nanotube-reduced graphene oxide nanoribbon composites. , 2015, Biosensors & bioelectronics.

[13]  Günter Mayer,et al.  A Versatile Approach Towards Nucleobase-Modified Aptamers. , 2015, Angewandte Chemie.

[14]  R. Breaker.  Riboswitches and the RNA world. , 2012, Cold Spring Harbor perspectives in biology.

[15]  Guohua Zhao,et al.  A simple and label-free aptasensor based on nickel hexacyanoferrate nanoparticles as signal probe for highly sensitive detection of 17β-estradiol. , 2015, Biosensors & bioelectronics.

[16]  P. Dubruel,et al.  Aptamer-Based Molecular Recognition of Lysergamine, Metergoline and Small Ergot Alkaloids , 2012, International journal of molecular sciences.

[17]  Mohamed Siaj,et al.  In vitro selection, characterization, and biosensing application of high-affinity cylindrospermopsin-targeting aptamers. , 2014, Analytical chemistry.

[18]  G. Mayer,et al.  The promise of riboswitches as potential antibacterial drug targets. , 2014, International journal of medical microbiology : IJMM.

[19]  Li Mu,et al.  ssDNA aptamer-based column for simultaneous removal of nanogram per liter level of illicit and analgesic pharmaceuticals in drinking water. , 2011, Environmental science & technology.

[20]  Taghi Khayamian,et al.  Anticodeine aptamer immobilized on a Whatman cellulose paper for thin-film microextraction of codeine from urine followed by electrospray ionization ion mobility spectrometry , 2015, Analytical and Bioanalytical Chemistry.

[21]  Rui Tian,et al.  A novel analytical probe binding to a potential carcinogenic factor of N-glycolylneuraminic acid by SELEX. , 2013, Biosensors & bioelectronics.

[22]  N. Teramae,et al.  Competitive assay for theophylline based on an abasic site-containing DNA duplex aptamer and a fluorescent ligand. , 2012, Chemistry.

[23]  Cuichen Wu,et al.  Engineering of switchable aptamer micelle flares for molecular imaging in living cells. , 2013, ACS nano.

[24]  Yunqing Liu,et al.  A novel electrochemical aptasensor for bisphenol A assay based on triple-signaling strategy. , 2016, Biosensors & bioelectronics.

[25]  B. Sullenger,et al.  Developing aptamers into therapeutics. , 2000, The Journal of clinical investigation.

[26]  D. Drolet,et al.  An enzyme-linked oligonucleotide assay , 1996, Nature Biotechnology.

[27]  Nuo Duan,et al.  Selection and identification of ssDNA aptamers recognizing zearalenone , 2013, Analytical and Bioanalytical Chemistry.

[28]  M. Roushani,et al.  Fabrication of an ultrasensitive ibuprofen nanoaptasensor based on covalent attachment of aptamer to electrochemically deposited gold-nanoparticles on glassy carbon electrode. , 2015, Talanta.

[29]  Guohua Zhao,et al.  A femtomolar level and highly selective 17β-estradiol photoelectrochemical aptasensor applied in environmental water samples analysis. , 2014, Environmental science & technology.

[30]  J. Piccirilli,et al.  A G-Quadruplex-Containing RNA Activates Fluorescence in a GFP-Like Fluorophore , 2014, Nature chemical biology.

[31]  Cuichen Wu,et al.  A general excimer signaling approach for aptamer sensors. , 2010, Biosensors & bioelectronics.

[32]  Ying Li,et al.  Aptamer-based fluorescent screening assay for acetamiprid via inner filter effect of gold nanoparticles on the fluorescence of CdTe quantum dots , 2015, Analytical and Bioanalytical Chemistry.

[33]  Coupling between substrate binding and allosteric regulation in ribozyme catalysis. , 2001, Bioorganic & medicinal chemistry.

[34]  Min Zhang,et al.  Quantum dot-DNA aptamer conjugates coupled with capillary electrophoresis: A universal strategy for ratiometric detection of organophosphorus pesticides. , 2016, Talanta.

[35]  Seunghun Hong,et al.  Aptamer sandwich-based carbon nanotube sensors for single-carbon-atomic-resolution detection of non-polar small molecular species. , 2011, Lab on a chip.

[36]  M. Carmell,et al.  Posttranscriptional Gene Silencing in Plants , 2006 .

[37]  Andrew D. Ellington,et al.  Surface-immobilized aptamers for cancer cell isolation and microscopic cytology. , 2010, Cancer research.

[38]  D. Patel,et al.  Encapsulating streptomycin within a small 40-mer RNA. , 2003, Chemistry & biology.

[39]  Amanda Deering,et al.  Melamine detection in infant formula powder using near- and mid-infrared spectroscopy. , 2009, Journal of agricultural and food chemistry.

[40]  Guohua Zhao,et al.  Aptamer-based colorimetric sensing of acetamiprid in soil samples: sensitivity, selectivity and mechanism. , 2013, Journal of hazardous materials.

[41]  Letha J. Sooter,et al.  In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil , 2014, Journal of nucleic acids.

[42]  Hui Li,et al.  An ultrasensitive homogeneous aptasensor for kanamycin based on upconversion fluorescence resonance energy transfer. , 2014, Biosensors & bioelectronics.

[43]  Ming Zhou,et al.  Solid-state probe based electrochemical aptasensor for cocaine: a potentially convenient, sensitive, repeatable, and integrated sensing platform for drugs. , 2010, Analytical chemistry.

[44]  M. Zourob,et al.  Aptamer-based label-free impedimetric biosensor for detection of progesterone. , 2015, Analytical chemistry.

[45]  W. Carmichael,et al.  Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil. , 2002, Toxicology.

[46]  G Marius Clore,et al.  Improving the accuracy of NMR structures of RNA by means of conformational database potentials of mean force as assessed by complete dipolar coupling cross-validation. , 2003, Journal of the American Chemical Society.

[47]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[48]  D. Guyer,et al.  Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease , 2006, Nature Reviews Drug Discovery.

[49]  Markus Wieland,et al.  Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells , 2011, Nucleic acids research.

[50]  Ning Gan,et al.  A "signal-on'' aptasensor for simultaneous detection of chloramphenicol and polychlorinated biphenyls using multi-metal ions encoded nanospherical brushes as tracers. , 2015, Biosensors & bioelectronics.

[51]  Maria C. DeRosa,et al.  Challenges and Opportunities for Small Molecule Aptamer Development , 2012, Journal of nucleic acids.

[52]  E. Maher,et al.  In vitro selection of a single-stranded DNA molecular recognition element for the pesticide malathion. , 2014, Combinatorial chemistry & high throughput screening.

[53]  J. Szostak,et al.  Isolation of a fluorophore-specific DNA aptamer with weak redox activity. , 1998, Chemistry & biology.

[54]  Brian A Sparano,et al.  A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. , 2005, Journal of the American Chemical Society.

[55]  Nadia Nikolaus,et al.  DNA-Aptamers Binding Aminoglycoside Antibiotics , 2014, Sensors.

[56]  Arica A Lubin,et al.  Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor. , 2009, Journal of the American Chemical Society.

[57]  S. Jaffrey,et al.  Designing optogenetically controlled RNA for regulating biological systems , 2015, Annals of the New York Academy of Sciences.

[58]  Shuxia Xu,et al.  Reusable light-emitting-diode induced chemiluminescence aptasensor for highly sensitive and selective detection of riboflavin. , 2013, Biosensors & bioelectronics.

[59]  Günter Mayer,et al.  β-Conglutin dual aptamers binding distinct aptatopes , 2015, Analytical and Bioanalytical Chemistry.

[60]  Su Jin Lee,et al.  ssDNA aptamers that recognize diclofenac and 2-anilinophenylacetic acid. , 2009, Bioorganic & medicinal chemistry.

[61]  M. Ramezani,et al.  A novel colorimetric triple-helix molecular switch aptasensor for ultrasensitive detection of tetracycline. , 2015, Biosensors & bioelectronics.

[62]  Changill Ban,et al.  Aptasensor for ampicillin using gold nanoparticle based dual fluorescence–colorimetric methods , 2012, Analytical and Bioanalytical Chemistry.

[63]  Ronghua Yang,et al.  Two-photon graphene oxide/aptamer nanosensing conjugate for in vitro or in vivo molecular probing. , 2014, Analytical chemistry.

[64]  Zhi Zhu,et al.  Monoclonal surface display SELEX for simple, rapid, efficient, and cost-effective aptamer enrichment and identification. , 2014, Analytical chemistry.

[65]  T. Ha.  Recent Advances for the Detection of Ochratoxin A , 2015, Toxins.

[66]  P. Labbé,et al.  Sensor Based on Aptamer Folding to Detect Low-Molecular Weight Analytes. , 2015, Analytical chemistry.

[67]  Xia Sun,et al.  Aptasensor based on the synergistic contributions of chitosan-gold nanoparticles, graphene-gold nanoparticles and multi-walled carbon nanotubes-cobalt phthalocyanine nanocomposites for kanamycin detection. , 2014, The Analyst.

[68]  Xi Chen,et al.  Determination of microcystin-LR in water by a label-free aptamer based electrochemical impedance biosensor. , 2013, Talanta.

[69]  C. Kao,et al.  Segmented GFP-like aptamer probes for functional imaging of viral genome trafficking. , 2015, Virus research.

[70]  K. Horii,et al.  Development of a Sphingosylphosphorylcholine Detection System Using RNA Aptamers , 2010, Molecules.

[71]  A. Ferré-D’Amaré,et al.  Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch. , 2008, Chemistry & biology.

[72]  Charles Wilson,et al.  The structural basis for molecular recognition by the vitamin B 12 RNA aptamer , 2000, Nature Structural Biology.

[73]  Jyun Yoshizumi,et al.  Target-induced strand release (TISR) from aptamer-DNA duplex: a general strategy for electronic detection of biomolecules ranging from a small molecule to a large protein. , 2008, The Analyst.

[74]  Noemí de-los-Santos-Álvarez,et al.  Aptamer-Based Analysis: A Promising Alternative for Food Safety Control , 2013, Sensors.

[75]  Q. Zhang,et al.  Multiplexed fluorescence resonance energy transfer aptasensor between upconversion nanoparticles and graphene oxide for the simultaneous determination of mycotoxins. , 2012, Analytical chemistry.

[76]  Nancy Kelley-Loughnane,et al.  Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol , 2014, Analytical and Bioanalytical Chemistry.

[77]  Qiang Wang,et al.  Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum. , 2015, Analytica chimica acta.

[78]  B. Mayosi,et al.  Isolation and characterization of 2'-F-RNA aptamers against whole HIV-1 subtype C envelope pseudovirus. , 2015, Biochemical and biophysical research communications.

[79]  Taekjip Ha,et al.  Tandem Spinach Array for mRNA Imaging in Living Bacterial Cells , 2015, Scientific Reports.

[80]  Y. S. Rajput,et al.  Selection of aptamers for aflatoxin M1 and their characterization , 2014, Journal of molecular recognition : JMR.

[81]  M. Gu,et al.  An ultra-sensitive colorimetric detection of tetracyclines using the shortest aptamer with highly enhanced affinity. , 2014, Chemical communications.

[82]  M. Green,et al.  Controlling gene expression in living cells through small molecule-RNA interactions. , 1998, Science.

[83]  Dinshaw J. Patel,et al.  Solution structure of the tobramycin–RNA aptamer complex , 1998, Nature Structural Biology.

[84]  Liguang Xu,et al.  Building an aptamer/graphene oxide FRET biosensor for one-step detection of bisphenol A. , 2015, ACS applied materials & interfaces.

[85]  Dipankar Sen,et al.  Reversible photo-regulation of a hammerhead ribozyme using a diffusible effector. , 2007, Journal of molecular biology.

[86]  A. Ferré-D’Amaré,et al.  Structural basis for activity of highly efficient RNA mimics of green fluorescent protein , 2014, Nature Structural &Molecular Biology.

[87]  Molly M Stevens,et al.  Colorimetric Detection of Small Molecules in Complex Matrixes via Target-Mediated Growth of Aptamer-Functionalized Gold Nanoparticles. , 2015, Analytical chemistry.

[88]  L. Sturla,et al.  Selection and characterization of single stranded DNA aptamers for the hormone abscisic Acid. , 2013, Nucleic acid therapeutics.

[89]  C. Ban,et al.  A coordination polymer nanobelt (CPNB)-based aptasensor for sulfadimethoxine. , 2012, Biosensors & bioelectronics.

[90]  A. Fire,et al.  Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans , 1998, Nature.

[91]  Roger A. Jones,et al.  Structural basis of RNA folding and recognition in an AMP–RNA aptamer complex , 1996, Nature.

[92]  Shenshan Zhan,et al.  Ultrasensitive resonance scattering (RS) spectral detection for trace tetracycline in milk using aptamer-coated nanogold (ACNG) as a catalyst. , 2014, Journal of agricultural and food chemistry.

[93]  Xiaohong Fang,et al.  Exonuclease I aided enzyme-linked aptamer assay for small-molecule detection , 2014, Analytical and Bioanalytical Chemistry.

[94]  J. Bruno,et al.  Development of DNA aptamers for cytochemical detection of acetylcholine , 2008, In Vitro Cellular & Developmental Biology - Animal.

[95]  Jack W. Szostak,et al.  An RNA motif that binds ATP , 1993, Nature.

[96]  James M Carothers,et al.  Solution structure of an informationally complex high-affinity RNA aptamer to GTP. , 2006, RNA.

[97]  Guohua Zhao,et al.  A highly selective electrochemical impedance spectroscopy-based aptasensor for sensitive detection of acetamiprid. , 2013, Biosensors & bioelectronics.

[98]  A. Deiters,et al.  Light‐Regulated RNA–Small Molecule Interactions , 2008, Chembiochem : a European journal of chemical biology.

[99]  Sergei A Kazakov,et al.  An RNA-aptamer-based assay for the detection and analysis of malachite green and leucomalachite green residues in fish tissue. , 2010, Analytical chemistry.

[100]  Gwendolyn M. Stovall,et al.  Technical and biological issues relevant to cell typing with aptamers. , 2009, Journal of proteome research.

[101]  Steven H. Y. Wong,et al.  Standards of laboratory practice: analgesic drug monitoring , 1998 .

[102]  Fluorescent aptasensors based on conformational adaptability of abasic site-containing aptamers in combination with abasic site-binding ligands. , 2011, Biosensors & bioelectronics.

[103]  Michel H M Eppink,et al.  Alternative affinity tools: more attractive than antibodies? , 2011, The Biochemical journal.

[104]  D. Patel,et al.  Saccharide-RNA recognition in an aminoglycoside antibiotic-RNA aptamer complex. , 1997, Chemistry & biology.

[105]  Grant R. Zimmermann,et al.  Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA , 1997, Nature Structural Biology.

[106]  Jadranka Travas-Sejdic,et al.  Label-free electrochemical aptasensor for femtomolar detection of 17β-estradiol. , 2015, Biosensors & bioelectronics.

[107]  J. Byun,et al.  A structure-switchable aptasensor for aflatoxin B1 detection based on assembly of an aptamer/split DNAzyme. , 2015, Analytica chimica acta.

[108]  M. Baker.  Reproducibility crisis: Blame it on the antibodies , 2015, Nature.

[109]  Mengmeng Yan,et al.  Gold nanoparticle–based colorimetric aptasensor for rapid detection of six organophosphorous pesticides , 2015, Environmental toxicology and chemistry.

[110]  C. Wilson,et al.  The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition. , 2000, Journal of molecular biology.

[111]  E. Ferapontova,et al.  An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. , 2008, Journal of the American Chemical Society.

[112]  A. Xu,et al.  Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis. , 2012, Analytical biochemistry.

[113]  L. Du,et al.  An ATP sensitive light addressable biosensor for extracellular monitoring of single taste receptor cell , 2012, Biomedical Microdevices.

[114]  J W Szostak,et al.  Isolation and characterization of fluorophore-binding RNA aptamers. , 1998, Folding & design.

[115]  M. Gu,et al.  Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip , 2007 .

[116]  Chang Ming Li,et al.  Aptamer induced assembly of fluorescent nitrogen-doped carbon dots on gold nanoparticles for sensitive detection of AFB1. , 2016, Biosensors & bioelectronics.

[117]  Yan Shi,et al.  Fluorescent sensing of cocaine based on a structure switching aptamer, gold nanoparticles and graphene oxide. , 2013, The Analyst.

[118]  Veli Cengiz Ozalp,et al.  Dual-polarization interferometry for quantification of small molecules using aptamers. , 2012, Analytical and bioanalytical chemistry.

[119]  Freddy Dardenne,et al.  Aptasensing of chloramphenicol in the presence of its analogues: reaching the maximum residue limit. , 2012, Analytical chemistry.

[120]  Nancy Kelley-Loughnane,et al.  Aptamer-functionalized nanoparticles for surface immobilization-free electrochemical detection of cortisol in a microfluidic device. , 2016, Biosensors & bioelectronics.

[121]  C. Reinemann,et al.  Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring. , 2016, Biosensors & bioelectronics.

[122]  Jing Li,et al.  A sensitive and label-free photoelectrochemical aptasensor using Co-doped ZnO diluted magnetic semiconductor nanoparticles. , 2016, Biosensors & bioelectronics.

[123]  Hongyan Jiang,et al.  Theophylline detection in serum using a self-assembling RNA aptamer-based gold nanoparticle sensor. , 2015, Biosensors & bioelectronics.

[124]  D. Wemmer,et al.  Structure determination and binding kinetics of a DNA aptamer-argininamide complex. , 2000, Biochemistry.

[125]  Mohamed Siaj,et al.  Label-free voltammetric aptasensor for the sensitive detection of microcystin-LR using graphene-modified electrodes. , 2014, Analytical chemistry.

[126]  M. He,et al.  Nanoprobe-Enhanced, Split Aptamer-Based Electrochemical Sandwich Assay for Ultrasensitive Detection of Small Molecules. , 2015, Analytical chemistry.

[127]  E. Gragoudas,et al.  Pegaptanib for neovascular age-related macular degeneration. , 2004, The New England journal of medicine.

[128]  A. Kuper.  Strategy for Development , 1977 .

[129]  Yaping Tian,et al.  Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey. , 2013, Talanta.

[130]  Xin Huang,et al.  Selection of Intracellularly Functional RNA Mimics of Green Fluorescent Protein Using Fluorescence-Activated Cell Sorting , 2015, Journal of Molecular Evolution.

[131]  Ming Zhou,et al.  G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: using magnetic nanoparticles as the separation and amplification element. , 2011, The Analyst.

[132]  Andres Jäschke,et al.  Contact-mediated quenching for RNA imaging in bacteria with a fluorophore-binding aptamer. , 2013, Angewandte Chemie.

[133]  D. Kolpashchikov.  Binary malachite green aptamer for fluorescent detection of nucleic acids. , 2005, Journal of the American Chemical Society.

[134]  Dihua Shangguan,et al.  A label-free electrochemical biosensor based on a DNA aptamer against codeine. , 2013, Analytica chimica acta.

[135]  J. Robbens,et al.  Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum. , 2014, Biosensors & bioelectronics.

[136]  Samie R. Jaffrey,et al.  RNA mimics of green fluorescent protein , 2013 .

[137]  G. Meng,et al.  Label-free selective SERS detection of PCB-77 based on DNA aptamer modified SiO₂@Au core/shell nanoparticles. , 2014, The Analyst.

[138]  Ke-Jing Huang,et al.  Aptamer/Au nanoparticles/cobalt sulfide nanosheets biosensor for 17β-estradiol detection using a guanine-rich complementary DNA sequence for signal amplification. , 2015, Biosensors & bioelectronics.

[139]  D. Patel,et al.  Saccharide-RNA recognition in a complex formed between neomycin B and an RNA aptamer. , 1999, Structure.

[140]  Zhen Zhao,et al.  A label-free electrochemical impedance aptasensor for cylindrospermopsin detection based on thionine-graphene nanocomposites. , 2015, The Analyst.

[141]  J. Vidal,et al.  Electrochemical affinity biosensors for detection of mycotoxins: A review. , 2013, Biosensors & bioelectronics.

[142]  Charles J Werth,et al.  Selective Aptamers for Detection of Estradiol and Ethynylestradiol in Natural Waters. , 2015, Environmental science & technology.

[143]  Y. Aoyama,et al.  Transcription monitoring using fused RNA with a dye-binding light-up aptamer as a tag: a blue fluorescent RNA. , 2008, Chemical communications.

[144]  G. Tocchini-Valentini,et al.  In vitro selection of dopamine RNA ligands. , 1997, Biochemistry.

[145]  Zifan Lu,et al.  An aptamer-based immunoassay in microchannels of a portable analyzer for detection of microcystin-leucine-arginine. , 2014, Talanta.

[146]  Rachel Ostroff,et al.  Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery , 2010, PLoS ONE.

[147]  Ayuob Aghanejad,et al.  Aptamer-based Nanosensors: Juglone as an Attached-Redox Molecule for Detection of Small Molecules. , 2011, BioImpacts : BI.

[148]  Erkang Wang,et al.  A electrochemiluminescence aptasensor for detection of thrombin incorporating the capture aptamer labeled with gold nanoparticles immobilized onto the thio-silanized ITO electrode , 2008 .

[149]  C. Wilson,et al.  2.8 A crystal structure of the malachite green aptamer. , 2000, Journal of molecular biology.

[150]  Jie Li,et al.  A novel label-free electrochemical aptasensor based on graphene-polyaniline composite film for dopamine determination. , 2012, Biosensors & bioelectronics.

[151]  M S Thakur,et al.  Functionalized aptamers as nano-bioprobes for ultrasensitive detection of bisphenol-A. , 2013, Chemical communications.

[152]  Jie Zhou,et al.  E88, a new cyclic-di-GMP class I riboswitch aptamer from Clostridium tetani, has a similar fold to the prototypical class I riboswitch, Vc2, but differentially binds to c-di-GMP analogs. , 2014, Molecular bioSystems.

[153]  Ya-Ting Chung,et al.  An ultrasensitive nanowire-transistor biosensor for detecting dopamine release from living PC12 cells under hypoxic stimulation. , 2013, Journal of the American Chemical Society.

[154]  Abhishek Phatarphekar,et al.  Determination of 1,25-dihydroxyvitamin D2 in rat serum using liquid chromatography with tandem mass spectrometry. , 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[155]  Zhengbo Chen,et al.  An electrochemical aptasensor based on the amplification of two kinds of gold nanocrystals for the assay of L-histidine with picomolar detection limit , 2013, Nanotechnology.

[156]  A.V. Lakhin,et al.  Aptamers: Problems, Solutions and Prospects , 2013, Acta naturae.

[157]  Byung-Kwan Cho,et al.  In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars , 2013, Biotechnology and bioengineering.

[158]  Yiyang Dong,et al.  Development of an indirect competitive assay-based aptasensor for highly sensitive detection of tetracycline residue in honey. , 2014, Biosensors & bioelectronics.

[159]  Zhengbo Chen,et al.  Electrochemical sensing of L-histidine based on structure-switching DNAzymes and gold nanoparticle-graphene nanosheet composites. , 2011, Chemical communications.

[160]  Jian-hui Jiang,et al.  A new aptameric biosensor for cocaine based on surface-enhanced Raman scattering spectroscopy. , 2008, Chemistry.

[161]  April Z Gu,et al.  A portable optic fiber aptasensor for sensitive, specific and rapid detection of bisphenol-A in water samples. , 2014, Environmental science. Processes & impacts.

[162]  Anne W. Taylor,et al.  Inadequate Riboflavin Intake and Anemia Risk in a Chinese Population: Five-Year Follow Up of the Jiangsu Nutrition Study , 2014, PloS one.

[163]  T. Tuschl,et al.  Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells , 2001, Nature.

[164]  Shin-ichi Sato,et al.  Small-molecule fluorescent probes for specific RNA targets. , 2011, Chemical communications.

[165]  J. Szostak,et al.  A DNA aptamer that binds adenosine and ATP. , 1995, Biochemistry.

[166]  R. Schroeder,et al.  In vitro selection and characterization of streptomycin-binding RNAs: recognition discrimination between antibiotics. , 1998, RNA.

[167]  Kayla Hill,et al.  Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation. , 2015, Analytical chemistry.

[168]  Razvan Nutiu,et al.  In vitro selection of structure-switching signaling aptamers. , 2005, Angewandte Chemie.

[169]  Andres Jäschke,et al.  Universal aptamer-based real-time monitoring of enzymatic RNA synthesis. , 2013, Journal of the American Chemical Society.

[170]  Eun Jeong Cho,et al.  Using a deoxyribozyme ligase and rolling circle amplification to detect a non-nucleic acid analyte, ATP. , 2005, Journal of the American Chemical Society.

[171]  M. Stojanović,et al.  Aptamer-based folding fluorescent sensor for cocaine. , 2001, Journal of the American Chemical Society.

[172]  G. Mayer,et al.  (Dis)similar Analogues of Riboswitch Metabolites as Antibacterial Lead Compounds. , 2015, Journal of medicinal chemistry.

[173]  Kevin W Plaxco,et al.  Real-Time, Aptamer-Based Tracking of Circulating Therapeutic Agents in Living Animals , 2013, Science Translational Medicine.

[174]  Feng Xue,et al.  Ultrasensitive one-step rapid visual detection of bisphenol A in water samples by label-free aptasensor. , 2013, Biosensors & bioelectronics.

[175]  E. Peyrin,et al.  Noncompetitive fluorescence polarization aptamer-based assay for small molecule detection. , 2009, Analytical chemistry.

[176]  A. Vallée-Bélisle,et al.  Using distal-site mutations and allosteric inhibition to tune, extend, and narrow the useful dynamic range of aptamer-based sensors. , 2012, Journal of the American Chemical Society.

[177]  A. Karkhane,et al.  In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin. , 2012, Analytica chimica acta.

[178]  E. Peyrin,et al.  Simple and highly enantioselective electrochemical aptamer-based binding assay for trace detection of chiral compounds. , 2012, Analytical chemistry.

[179]  I. R. Paeng,et al.  Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum. , 2011, Analytica chimica acta.

[180]  Yoshikazu Nakamura,et al.  A binary Cy3 aptamer probe composed of folded modules. , 2010, Analytical biochemistry.

[181]  L. Selvakumar,et al.  Nano RNA aptamer wire for analysis of vitamin B₁₂. , 2012, Analytical biochemistry.

[182]  M. Bowser,et al.  Capillary electrophoresis-SELEX selection of catalytic DNA aptamers for a small-molecule porphyrin target. , 2013, Analytical chemistry.

[183]  Mohammad Ramezani,et al.  Design and fabrication of an aptasensor for chloramphenicol based on energy transfer of CdTe quantum dots to graphene oxide sheet. , 2015, Materials science & engineering. C, Materials for biological applications.

[184]  Wade W Grabow,et al.  Fluorescent monitoring of RNA assembly and processing using the split-spinach aptamer. , 2015, ACS synthetic biology.

[185]  M. Ganjali,et al.  Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples. , 2015, Acta chimica Slovenica.

[186]  Kevin W Plaxco,et al.  Reagentless measurement of aminoglycoside antibiotics in blood serum via an electrochemical, ribonucleic acid aptamer-based biosensor. , 2010, Analytical chemistry.

[187]  Shusheng Zhang,et al.  Electrochemical biosensor for detection of adenosine based on structure-switching aptamer and amplification with reporter probe DNA modified Au nanoparticles. , 2008, Analytical chemistry.

[188]  A. Pardi,et al.  High-resolution molecular discrimination by RNA. , 1994, Science.

[189]  Mohammad Ramezani,et al.  Colorimetric and fluorescence quenching aptasensors for detection of streptomycin in blood serum and milk based on double-stranded DNA and gold nanoparticles. , 2016, Food chemistry.

[190]  E. Peyrin,et al.  Capillary gel electrophoresis-coupled aptamer enzymatic cleavage protection strategy for the simultaneous detection of multiple small analytes. , 2014, Analytical chemistry.

[191]  C. Ban,et al.  Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer. , 2011, Analytical biochemistry.

[192]  Qixing Zhou,et al.  Immobilized smart RNA on graphene oxide nanosheets to specifically recognize and adsorb trace peptide toxins in drinking water. , 2012, Journal of hazardous materials.

[193]  Yanbin Li,et al.  An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol A in milk samples. , 2014, Food chemistry.

[194]  Danke Xu,et al.  Aptamer/Polydopamine Nanospheres Nanocomplex for in Situ Molecular Sensing in Living Cells. , 2015, Analytical chemistry.

[195]  Lili He,et al.  Development of a single aptamer-based surface enhanced Raman scattering method for rapid detection of multiple pesticides. , 2014, The Analyst.

[196]  Modi Wang,et al.  A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex , 2013, PloS one.

[197]  Jiyong Zhou,et al.  RNA aptamer based electrochemical biosensor for sensitive and selective detection of cAMP. , 2015, Biosensors & bioelectronics.

[198]  Samie R Jaffrey,et al.  In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. , 2015, Chemistry & biology.

[199]  Ping Yu,et al.  Dual recognition unit strategy improves the specificity of the adenosine triphosphate (ATP) aptamer biosensor for cerebral ATP assay. , 2015, Analytical chemistry.

[200]  Liqiang Liu,et al.  Asymmetric plasmonic aptasensor for sensitive detection of bisphenol A. , 2014, ACS applied materials & interfaces.

[201]  Carlotta Guiducci,et al.  Label-free detection of tobramycin in serum by transmission-localized surface plasmon resonance. , 2015, Analytical chemistry.

[202]  Xiaohong Zhou,et al.  Label-free detection of kanamycin based on a G-quadruplex DNA aptamer-based fluorescent intercalator displacement assay , 2015, Scientific Reports.

[203]  He Zhang,et al.  Aptamer-based microfluidic beads array sensor for simultaneous detection of multiple analytes employing multienzyme-linked nanoparticle amplification and quantum dots labels. , 2014, Biosensors & bioelectronics.

[204]  Paul A. Wiggins,et al.  RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking. , 2014, ACS chemical biology.

[205]  Grigory S. Filonov,et al.  Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution , 2014, Journal of the American Chemical Society.

[206]  Jaehoon Yu,et al.  In vitro selection of RNA aptamers that selectively bind danofloxacin. , 2014, Biochemical and biophysical research communications.

[207]  April Z Gu,et al.  Aptamer-based optical biosensor for rapid and sensitive detection of 17β-estradiol in water samples. , 2012, Environmental science & technology.

[208]  Peiwu Li,et al.  IMMUNOASSAYS FOR AFLATOXINS , 2009 .

[209]  Li Jianlin,et al.  Simultaneous detection of ochratoxin A and fumonisin B1 in cereal samples using an aptamer-photonic crystal encoded suspension array. , 2014, Analytical chemistry.

[210]  Zhouping Wang,et al.  Screening and identification of DNA aptamers against T-2 toxin assisted by graphene oxide. , 2014, Journal of agricultural and food chemistry.

[211]  Mohammad Ramezani,et al.  A novel electrochemical aptasensor based on arch-shape structure of aptamer-complimentary strand conjugate and exonuclease I for sensitive detection of streptomycin. , 2016, Biosensors & bioelectronics.

[212]  Shuping Xu,et al.  Aptamer-based surface-enhanced Raman scattering-microfluidic sensor for sensitive and selective polychlorinated biphenyls detection. , 2015, Analytical chemistry.

[213]  M. Scippo,et al.  Selection and characterization of PCB-binding DNA aptamers. , 2012, Analytical chemistry.

[214]  Tracy R. Keeney,et al.  Aptamer-based multiplexed proteomic technology for biomarker discovery , 2010, Nature Precedings.

[215]  M Porta,et al.  Implementing the Stockholm Treaty on Persistent Organic Pollutants , 2002, Occupational and environmental medicine.

[216]  Kun Han,et al.  Design Strategies for Aptamer-Based Biosensors , 2010, Italian National Conference on Sensors.

[217]  Beatrix Suess,et al.  Identification of RNA aptamers with riboswitching properties. , 2016, Methods.

[218]  Shungui Zhou,et al.  Label-free DNA Y junction for bisphenol A monitoring using exonuclease III-based signal protection strategy. , 2016, Biosensors & bioelectronics.

[219]  T. Mészáros,et al.  Selection and Characterization of a Novel DNA Aptamer for Label-Free Fluorescence Biosensing of Ochratoxin A , 2014, Toxins.

[220]  Xiaodong Guo,et al.  Development of an ultrasensitive aptasensor for the detection of aflatoxin B1. , 2014, Biosensors & bioelectronics.

[221]  Eun Jeong Cho,et al.  Applications of aptamers as sensors. , 2009, Annual review of analytical chemistry.

[222]  Y. Okuno,et al.  Allosteric regulation of a ribozyme activity through ligand-induced conformational change. , 1998, Nucleic acids research.

[223]  Damian Labuda,et al.  In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines. , 2004, Biochemical and biophysical research communications.

[224]  Yiyang Dong,et al.  A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in honey. , 2015, Talanta.

[225]  M. A. Mohd,et al.  Joint FAO/WHO Expert Meeting to Review Toxicological and Health Aspects of Bisphenol A - Summary Report , 2010 .

[226]  S. Klußmann,et al.  Identification and characterization of a mirror-image oligonucleotide that binds and neutralizes sphingosine 1-phosphate, a central mediator of angiogenesis , 2014, The Biochemical journal.

[227]  E. Ibáñez,et al.  Present and future challenges in food analysis: foodomics. , 2012, Analytical chemistry.

[228]  Mohamed Siaj,et al.  DNA aptamers selection and characterization for development of label-free impedimetric aptasensor for neurotoxin anatoxin-a. , 2015, Biosensors & bioelectronics.

[229]  J. Boulanger,et al.  Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast , 2015, Nature Communications.

[230]  V. C. Özalp,et al.  Time-resolved Measurements of Intracellular ATP in the Yeast Saccharomyces cerevisiae using a New Type of Nanobiosensor* , 2010, The Journal of Biological Chemistry.

[231]  C. Wilson,et al.  A water channel in the core of the vitamin B(12) RNA aptamer. , 2000, Structure.

[232]  A. Ellington,et al.  Adapting selected nucleic acid ligands (aptamers) to biosensors. , 1998, Analytical chemistry.

[233]  Omar A Alsager,et al.  Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles. , 2014, Biosensors & bioelectronics.

[234]  Min-Gon Kim,et al.  An aptamer-based dipstick assay for the rapid and simple detection of aflatoxin B1. , 2014, Biosensors & bioelectronics.

[235]  V. Hornung,et al.  Ni2+-binding RNA motifs with an asymmetric purine-rich internal loop and a G-A base pair. , 1997, RNA.

[236]  Vijayender Bhalla,et al.  Nanobioprobe mediated DNA aptamers for explosive detection. , 2014, Chemical communications.

[237]  S. Piletsky,et al.  Analytical methods for determination of mycotoxins: An update (2009-2014). , 2015, Analytica chimica acta.

[238]  M. Zourob,et al.  Selection and identification of DNA aptamers against okadaic acid for biosensing application. , 2013, Analytical chemistry.

[239]  Andres Jäschke,et al.  Dual-colour imaging of RNAs using quencher- and fluorophore-binding aptamers , 2015, Nucleic acids research.

[240]  Wenjiao Song,et al.  Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria , 2013, Nature Protocols.

[241]  A. Frankel,et al.  Identification of two novel arginine binding DNAs. , 1995, The EMBO journal.

[242]  Michael Famulok,et al.  Aptamers and SELEX in Chemistry & Biology. , 2014, Chemistry & biology.

[243]  D. Patel,et al.  Molecular recognition in the FMN-RNA aptamer complex. , 1996, Journal of molecular biology.

[244]  T. Ellis,et al.  Using Spinach aptamer to correlate mRNA and protein levels in Escherichia coli. , 2015, Methods in enzymology.

[245]  Shuo Ren,et al.  Sol-gel SELEX circumventing chemical conjugation of low molecular weight metabolites discovers aptamers selective to xanthine. , 2013, Nucleic acid therapeutics.

[246]  Volker A. Erdmann,et al.  Biosensor-based on-site explosives detection using aptamers as recognition elements , 2008, Analytical and bioanalytical chemistry.

[247]  George A. Kraus,et al.  Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters , 2014, Nucleic acids research.

[248]  Ning Gan,et al.  A novel "dual-potential" electrochemiluminescence aptasensor array using CdS quantum dots and luminol-gold nanoparticles as labels for simultaneous detection of malachite green and chloramphenicol. , 2015, Biosensors & bioelectronics.

[249]  Ming Zhou,et al.  Microfluidic electrochemical aptameric assay integrated on-chip: a potentially convenient sensing platform for the amplified and multiplex analysis of small molecules. , 2011, Analytical chemistry.

[250]  E Westhof,et al.  Structural Basis of Ligand Discrimination by Two Related RNA Aptamers Resolved by NMR Spectroscopy , 1996, Science.

[251]  Juewen Liu,et al.  A simple and sensitive "dipstick" test in serum based on lateral flow separation of aptamer-linked nanostructures. , 2006, Angewandte Chemie.

[252]  J. Wower,et al.  Therapeutic RNA aptamers in clinical trials. , 2013, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[253]  J. Richard,et al.  Some major mycotoxins and their mycotoxicoses--an overview. , 2007, International journal of food microbiology.

[254]  M. Nilsen-Hamilton,et al.  Allosteric aptamers: targeted reversibly attenuated probes. , 2005, Biochemistry.

[255]  Huangxian Ju,et al.  Aptamer loaded MoS2 nanoplates as nanoprobes for detection of intracellular ATP and controllable photodynamic therapy. , 2015, Nanoscale.

[256]  Jinsong Ding,et al.  Intracellular detection of ATP using an aptamer beacon covalently linked to graphene oxide resisting nonspecific probe displacement. , 2014, Analytical chemistry.

[257]  Jishan Li,et al.  Dual-stimuli responsive i-motif/nanoflares for sensing ATP in lysosomes. , 2014, The Analyst.

[258]  Qijin Wan,et al.  High-Performance Electrochemical Catalysts Based on Three-Dimensional Porous Architecture with Conductive Interconnected Networks. , 2016, ACS applied materials & interfaces.

[259]  Vijayender Bhalla,et al.  Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene. , 2014, Analytical chemistry.

[260]  Guo-Li Shen,et al.  Fluorescence aptameric sensor for strand displacement amplification detection of cocaine. , 2010, Analytical chemistry.

[261]  L. Gold,et al.  RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[262]  Yi Lu,et al.  Portable Detection of Melamine in Milk Using a Personal Glucose Meter Based on an in Vitro Selected Structure-Switching Aptamer. , 2015, Analytical chemistry.

[263]  J. Szostak,et al.  Functional requirements for specific ligand recognition by a biotin-binding RNA pseudoknot. , 1998, Biochemistry.

[264]  Mohamed Siaj,et al.  Aptamer-based competitive electrochemical biosensor for brevetoxin-2. , 2015, Biosensors & bioelectronics.

[265]  Nadia Nikolaus,et al.  Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics , 2012, Journal of analytical methods in chemistry.

[266]  Sang-Hee Jeong,et al.  An indirect competitive assay-based aptasensor for detection of oxytetracycline in milk. , 2014, Biosensors & bioelectronics.

[267]  Shiru Jia,et al.  Fast determination of the tetracyclines in milk samples by the aptamer biosensor. , 2010, The Analyst.

[268]  C. Danelon,et al.  Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells. , 2015, Methods in enzymology.

[269]  Katrina Campbell,et al.  First report of the use of a saxitoxin-protein conjugate to develop a DNA aptamer to a small molecule toxin. , 2013, Toxicon : official journal of the International Society on Toxinology.

[270]  A. Kumudha,et al.  Methylcobalamin--a form of vitamin B12 identified and characterised in Chlorella vulgaris. , 2015, Food chemistry.

[271]  D. Patel,et al.  Structural basis of DNA folding and recognition in an AMP-DNA aptamer complex: distinct architectures but common recognition motifs for DNA and RNA aptamers complexed to AMP. , 1997, Chemistry & biology.

[272]  María Jesús Lobo-Castañón,et al.  Modified-RNA aptamer-based sensor for competitive impedimetric assay of neomycin B. , 2007, Journal of the American Chemical Society.

[273]  J. Marty,et al.  Aptamers: A Promosing Tool for Ochratoxin A Detection in Food Analysis , 2013, Toxins.

[274]  B. Hu,et al.  A saxitoxin-binding aptamer with higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. , 2015, Toxicon : official journal of the International Society on Toxinology.

[275]  J. Wengel,et al.  Multifunctional nucleic acids for tumor cell treatment. , 2014, Nucleic acid therapeutics.

[276]  Photoregulation of a peptide-RNA interaction on a gold surface. , 2007, Journal of the American Chemical Society.

[277]  J S Moore,et al.  Design and construction. , 1972, Hospitals.

[278]  J. Tait,et al.  Challenges and opportunities. , 1996, Journal of psychiatric and mental health nursing.

[279]  BaeHyunjung,et al.  Sol-Gel SELEX Circumventing Chemical Conjugation of Low Molecular Weight Metabolites Discovers Aptamers Selective to Xanthine , 2013 .

[280]  J. Hodgkiss,et al.  Ultrasensitive colorimetric detection of 17β-estradiol: the effect of shortening DNA aptamer sequences. , 2015, Analytical chemistry.

[281]  Guonan Chen,et al.  Label-free aptamer-based electrochemical impedance biosensor for 17β-estradiol. , 2012, The Analyst.

[282]  Michael Famulok,et al.  Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures , 2010, Nature Protocols.

[283]  Seunghun Hong,et al.  Development of single-stranded DNA aptamers for specific Bisphenol a detection. , 2011, Oligonucleotides.

[284]  Qinghua He,et al.  Organophosphorus pesticides detection using broad-specific single-stranded DNA based fluorescence polarization aptamer assay. , 2014, Biosensors & bioelectronics.

[285]  Charles Wilson,et al.  Recognition of Planar and Nonplanar Ligands in the Malachite Green–RNA Aptamer Complex , 2004, Chembiochem : a European journal of chemical biology.

[286]  He Li,et al.  Colorimetric detection of microcystin-LR based on disassembly of orient-aggregated gold nanoparticle dimers. , 2015, Biosensors & bioelectronics.

[287]  Juewen Liu,et al.  Functional nucleic acid sensors. , 2009, Chemical reviews.

[288]  Weihong Tan,et al.  Semiquantification of ATP in live cells using nonspecific desorption of DNA from graphene oxide as the internal reference. , 2012, Analytical chemistry.

[289]  N. de-los-Santos-Álvarez,et al.  Impedimetric aptasensor for tobramycin detection in human serum. , 2011, Biosensors & bioelectronics.

[290]  D. Patel,et al.  Formation of an amino-acid-binding pocket through adaptive zippering-up of a large DNA hairpin loop. , 1998, Chemistry & biology.

[291]  Huy L. Nguyen,et al.  Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor. , 2013, Materials science & engineering. C, Materials for biological applications.

[292]  A. Heeger,et al.  An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids. , 2006, Journal of the American Chemical Society.

[293]  Xiliang Luo,et al.  A bioresponsive controlled-release bioassay based on aptamer-gated Au nanocages and its application in living cells. , 2015, Chemical communications.

[294]  G. F. Joyce,et al.  Amplification, mutation and selection of catalytic RNA. , 1989, Gene.

[295]  Guangchao Zhao,et al.  A theophylline quartz crystal microbalance biosensor based on recognition of RNA aptamer and amplification of signal. , 2013, The Analyst.

[296]  J. Choo,et al.  Surface-enhanced Raman scattering aptasensor for ultrasensitive trace analysis of bisphenol A. , 2015, Biosensors & bioelectronics.

[297]  Zhenyu Lin,et al.  Determination of cocaine on banknotes through an aptamer-based electrochemiluminescence biosensor , 2011, Analytical and bioanalytical chemistry.

[298]  M. Win,et al.  Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay , 2006, Nucleic acids research.

[299]  T. Noguer,et al.  Development of an impedimetric aptasensor for the determination of aflatoxin M1 in milk. , 2016, Talanta.

[300]  Development of photoswitchable RNA aptamer-ligand complexes. , 2014, Methods in molecular biology.

[301]  M. A. Mehrgardi,et al.  Design and construction of a label free aptasensor for electrochemical detection of sodium diclofenac. , 2012, Biosensors & bioelectronics.

[302]  Zhi Zhu,et al.  Target-responsive DNA hydrogel mediated "stop-flow" microfluidic paper-based analytic device for rapid, portable and visual detection of multiple targets. , 2015, Analytical chemistry.

[303]  L. Michiels,et al.  Aptamers targeting different functional groups of 17β-estradiol , 2015, The Journal of Steroid Biochemistry and Molecular Biology.