In vitro analysis of cryopreserved alginate–poly‐l‐lysine–alginate‐microencapsulated human hepatocytes

Background: The availability of well‐characterized human hepatocytes that can be frozen and thawed will be critical for cell therapy. We addressed whether human hepatocytes can recover after microencapsulated cryopreservation and investigated whether these cryopreserved microencapsulated hepatocytes can be used for clinical applications.

[1]  D. Emerich,et al.  Intraperitoneal stability of alginate-polyornithine microcapsules in rats: an FTIR and SEM analysis. , 2006, Biomaterials.

[2]  Zhangqi Feng,et al.  In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds. , 2009, Biomaterials.

[3]  A. Dhawan,et al.  Cryopreservation of isolated human hepatocytes for transplantation: State of the art. , 2006, Cryobiology.

[4]  M. Reth,et al.  Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination. , 2007, Journal of hepatology.

[5]  Rakhee Patel,et al.  Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study , 2004, Liver international : official journal of the International Association for the Study of the Liver.

[6]  M. Kusano,et al.  Microencapsule technique protects hepatocytes from cryoinjury , 2008, Hepatology research : the official journal of the Japan Society of Hepatology.

[7]  D. Emerich,et al.  Stability of alginate-polyornithine microcapsules is profoundly dependent on the site of transplantation. , 2007, Journal of biomedical materials research. Part A.

[8]  C. Guha,et al.  Amplification of engrafted hepatocytes by preparative manipulation of the host liver. , 2001, Artificial organs.

[9]  Y. Ilan Towards a bank of cryopreserved hepatocytes: which cell to freeze? , 2002, Journal of hepatology.

[10]  Yitao Ding,et al.  Morphology, Viability and Functions of Suckling Pig Hepatocytes Cultured in Serum-Free Medium at High Density , 2002, Digestive Surgery.

[11]  Peter Neuhaus,et al.  Cryopreservation of primary human hepatocytes: The benefit of trehalose as an additional cryoprotective agent , 2007, Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society.

[12]  M. Kusano,et al.  Long-Term Maintenance of the Drug Transport Activity in Cryopreservation of Microencapsulated Rat Hepatocytes , 2007, Cell transplantation.

[13]  A. Guillouzo,et al.  Hypothermic Storage and Cryopreservation of Hepatocytes: The Protective Effect of Alginate Gel against Cell Damages , 2003, Cell transplantation.

[14]  H. Zhang,et al.  Cryopreservation of suckling pig hepatocytes. , 2001, Annals of clinical and laboratory science.

[15]  I. Sauer,et al.  Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen. , 2008, Artificial organs.

[16]  Yitao Ding,et al.  Heterotypic interactions in the preservation of morphology and functionality of porcine hepatocytes by bone marrow mesenchymal stem cells in vitro , 2009, Journal of cellular physiology.

[17]  F. Oesch,et al.  Hepatocytes cultured in alginate microspheres: an optimized technique to study enzyme induction. , 2005, Toxicology.

[18]  Anil Dhawan,et al.  Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing , 2005, Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society.

[19]  M. Pokrywczyńska,et al.  Alginate is not a good material for growth of rapidly proliferating cells. , 2008, Transplantation proceedings.

[20]  De-cai Yu,et al.  Differentiation of embryoid-body cells derived from embryonic stem cells into hepatocytes in alginate microbeads in vitro , 2007, Acta Pharmacologica Sinica.

[21]  J. Pedraz,et al.  Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts. , 2009, Biomaterials.

[22]  A. Guillouzo,et al.  Viability and drug metabolism capacity of alginate-entrapped hepatocytes after cryopreservation , 2004, Cell Biology and Toxicology.

[23]  Yitao Ding,et al.  Bioartificial liver inoculated with porcine hepatocyte spheroids for treatment of canine acute liver failure model. , 2003, Artificial organs.

[24]  B. Davidson,et al.  Isolation of human hepatocytes after hepatic warm and cold ischemia: a practical approach using University of Wisconsin solution. , 1999, Cryobiology.

[25]  B. Kulseng,et al.  Transplantation of alginate microcapsules: generation of antibodies against alginates and encapsulated porcine islet-like cell clusters. , 1999, Transplantation.

[26]  V. Dixit,et al.  The bioartificial liver: state-of-the-art. , 2003, The European journal of surgery. Supplement. : = Acta chirurgica. Supplement.

[27]  S. Cereghini Liver‐enriched transcription factors and hepatocyte differentiation , 1996, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[28]  B. Lacarelle,et al.  Thawed human hepatocytes in primary culture. , 1992, Cryobiology.

[29]  M. Zern,et al.  Telomerase reconstitution immortalizes human fetal hepatocytes without disrupting their differentiation potential. , 2003, Gastroenterology.

[30]  P. Bachellier,et al.  Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. , 2002, Cryobiology.

[31]  Xiaolei Shi,et al.  A Simple Isolation and Cryopreservation Method for Adult Human Hepatocytes , 2009, The International journal of artificial organs.

[32]  R Williams,et al.  Pilot‐controlled trial of the extracorporeal liver assist device in acute liver failure , 1996, Hepatology.

[33]  M. Sherman,et al.  Development and evaluation of a system of microencapsulation of primary rat hepatocytes , 1989, Hepatology.

[34]  M. Kusano,et al.  A Novel Method of Cryopreservation of Rat and Human Hepatocytes by Using Encapsulation Technique and Possible Use for Cell Transplantation , 2005, Cell transplantation.

[35]  H. Sitter,et al.  Amitogenic Alginates: Key to First Clinical Application of Microencapsulation Technology , 1998, World Journal of Surgery.

[36]  A. Nussler,et al.  A New Technique for Isolating and Culturing Human Hepatocytes from Whole or Split Livers not Used for Transplantation , 1994, Cell transplantation.