A fluorescence-based microbial sensor for the selective detection of gold.

This study presents a fluorescence-based microbial sensor for the detection of metal ions as a novel analytical tool for environmental applications. Our results demonstrate the effectiveness of whole-cell sensors in the selective detection of gold ions. Two heavy-metal-tolerant proteobacteria, Cupriavidus metallidurans and Ralstonia eutropha, were examined and showed great specificity. This work highlights the potential of employing engineered microbial strains as robust analytical tools.

[1]  Tim Lincoln,et al.  Chemical communications , 1992, Nature.

[2]  N. Brown,et al.  The MerR family of transcriptional regulators. , 2003, FEMS microbiology reviews.

[3]  M. Mergeay,et al.  Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network , 2011, BioMetals.

[4]  Steven W. Singer,et al.  Engineering of Ralstonia eutropha H16 for Autotrophic and Heterotrophic Production of Methyl Ketones , 2013, Applied and Environmental Microbiology.

[5]  G. Grass,et al.  Contributions of Five Secondary Metal Uptake Systems to Metal Homeostasis of Cupriavidus metallidurans CH34 , 2011, Journal of bacteriology.

[6]  G. Hause,et al.  Influence of Copper Resistance Determinants on Gold Transformation by Cupriavidus metallidurans Strain CH34 , 2013, Journal of bacteriology.

[7]  Sylvia Daunert,et al.  Fluorescence-based sensing system for copper using genetically engineered living yeast cells. , 2004, Biotechnology and bioengineering.

[8]  Peng R. Chen,et al.  Engineering a gold-specific regulon for cell-based visual detection and recovery of gold , 2012 .

[9]  Anil Kumar,et al.  An Effective Strategy for a Whole-Cell Biosensor Based on Putative Effector Interaction Site of the Regulatory DmpR Protein , 2012, PloS one.

[10]  Gary L. Andersen,et al.  Caulobacter crescentus as a Whole-Cell Uranium Biosensor , 2007, Applied and Environmental Microbiology.

[11]  F. C. Soncini,et al.  Bacterial gold sensing and resistance , 2011, BioMetals.

[12]  M. Maher,et al.  Unprecedented binding cooperativity between Cu(I) and Cu(II) in the copper resistance protein CopK from Cupriavidus metallidurans CH34: implications from structural studies by NMR spectroscopy and X-ray crystallography. , 2009, Journal of the American Chemical Society.

[13]  Matias D Zurbriggen,et al.  Bacterial signaling systems as platforms for rational design of new generations of biosensors. , 2012, Current opinion in biotechnology.

[14]  F. C. Soncini,et al.  GolS controls the response to gold by the hierarchical induction of Salmonella‐specific genes that include a CBA efflux‐coding operon , 2007, Molecular microbiology.

[15]  F. C. Soncini,et al.  Target transcription binding sites differentiate two groups of MerR‐monovalent metal ion sensors , 2010, Molecular microbiology.

[16]  A. Sinskey,et al.  Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. , 2012, Sub-cellular biochemistry.

[17]  J. Keasling,et al.  Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids , 2012, Nature Biotechnology.

[18]  M. R. Binet,et al.  Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal‐transporting P‐type ATPase ZntA in Escherichia coli , 2000, FEBS letters.

[19]  Kiyohito Yagi,et al.  Applications of whole-cell bacterial sensors in biotechnology and environmental science , 2007, Applied Microbiology and Biotechnology.

[20]  N. Hillson,et al.  Functionalizing bacterial cell surfaces with a phage protein. , 2013, Chemical communications.

[21]  M Mergeay,et al.  A microbial biosensor to predict bioavailable nickel in soil and its transfer to plants. , 2001, Environmental pollution.

[22]  Erik C. Wasinger,et al.  Highly sensitive and selective gold(I) recognition by a metalloregulator in Ralstonia metallidurans. , 2009, Journal of the American Chemical Society.

[23]  Stefan Vogt,et al.  Mechanisms of gold biomineralization in the bacterium Cupriavidus metallidurans , 2009, Proceedings of the National Academy of Sciences.

[24]  F. C. Soncini,et al.  Selective detection of gold using genetically engineered bacterial reporters , 2011, Biotechnology and bioengineering.