Preparation of poly(3,4-ethylenedioxythiophene) nanofibers modified pencil graphite electrode and investigation of over-oxidation conditions for the selective and sensitive determination of uric acid in body fluids.

In this study, we have performed the preparation of over-oxidized poly(3,4-ethylenedioxythiophene) nanofibers modified pencil graphite electrode (Ox-PEDOT-nf/PGE) to develop a selective and sensitive voltammetric uric acid (UA) sensor. It was noted that the over-oxidation potential and time had a prominent effect on the UA response of the Ox-PEDOT-nf/PGE. Characterizations of PEDOT-nf/PGE and Ox-PEDOT-nf/PGE have been performed by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy. The highest voltammetric response of UA was obtained at pH 2.0. A linear relationship between the concentration of UA and oxidation peak currents was observed in the concentration range of 0.01-20.0 μM. The detection limit (1.3 nM according to S/N = 3) and reproducibility (RSD: 4.6 % for N:10) have also been determined. The effects of different substances on the determination of UA have been investigated. A very high peak separation value of 423 mV was obtained between UA and ascorbic acid which is the major interfering substance for UA. The use of Ox-PEDOT-nf/PGE has been successfully tested in the determination of UA in human blood serum and urine samples for the first time in the literature.

[1]  Liu Deng,et al.  Redox-active thionine-graphene oxide hybrid nanosheet: one-pot, rapid synthesis, and application as a sensing platform for uric acid. , 2013, Analytica chimica acta.

[2]  Jingkun Xu,et al.  Fabrication of nanostructured PEDOT clusters using β-cyclodextrin as substrate and applied for simultaneous determination of hyperoside and shikonin , 2015 .

[3]  T. Chakrabarty,et al.  Investigation of polyelectrolyte for electrochemical detection of uric acid in presence of ascorbic acid , 2012 .

[4]  Wei-Ting Chang,et al.  Strong adsorption characteristics of a novel overoxidized poly(3,4-ethylenedioxythiophene) film and application for dopamine sensing , 2014 .

[5]  Xusheng Du,et al.  Effects of polymerization potential on the properties of electrosynthesized PEDOT films , 2003 .

[6]  Y. Zuo,et al.  Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography. , 2011, Talanta.

[7]  Weihua Cai,et al.  Electrochemical determination of ascorbic acid, dopamine and uric acid based on an exfoliated graphite paper electrode: A high performance flexible sensor , 2014 .

[8]  Chang Wei,et al.  Graphitic carbon nitride nanosheets doped graphene oxide for electrochemical simultaneous determination of ascorbic acid, dopamine and uric acid , 2014 .

[9]  S. B. Revin,et al.  Highly sensitive determination of uric acid in the presence of major interferents using a conducting polymer film modified electrode. , 2012, Bioelectrochemistry.

[10]  M. Ozsoz,et al.  Electrochemical Oxidation of ds‐DNA on Polypyrrole Nanofiber Modified Pencil Graphite Electrode , 2007 .

[11]  Yangping Wen,et al.  A facile one-step redox route for the synthesis of graphene/poly (3,4-ethylenedioxythiophene) nanocomposite and their applications in biosensing , 2013 .

[12]  T. E. M. Nancy,et al.  Synergistic electrocatalytic effect of graphene/nickel hydroxide composite for the simultaneous electrochemical determination of ascorbic acid, dopamine and uric acid , 2014 .

[13]  L. Gorton,et al.  Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin. , 2013, Colloids and surfaces. B, Biointerfaces.

[14]  Jingkun Xu,et al.  Electroactive species-doped poly(3,4-ethylenedioxythiophene) films: enhanced sensitivity for electrochemical simultaneous determination of vitamins B2, B6 and C. , 2013, Biosensors & bioelectronics.

[15]  A. Galal,et al.  Poly(3,4-ethylene-dioxythiophene) electrode for the selective determination of dopamine in presence of sodium dodecyl sulfate. , 2011, Bioelectrochemistry.

[16]  B. Habibi,et al.  Simultaneous determination of ascorbic acid, dopamine and uric acid by use of a MWCNT modified carbon-ceramic electrode and differential pulse voltammetry , 2010 .

[17]  Ali Özcan,et al.  A novel approach for the determination of paracetamol based on the reduction of N-acetyl-p-benzoquinoneimine formed on the electrochemically treated pencil graphite electrode. , 2011, Analytica chimica acta.

[18]  Lei Jiang,et al.  Polymer in situ embedding for highly flexible, stretchable and water stable PEDOT:PSS composite conductors , 2013 .

[19]  Jixiao Wang,et al.  Polypyrrole nanostructures formed by electrochemical method on graphite impregnated with paraffin , 2006 .

[20]  Xiliang Luo,et al.  Carbon nanotube doped poly(3,4-ethylenedioxythiophene) for the electrocatalytic oxidation and detection of hydroquinone , 2013 .

[21]  Xiao-fei Zhu,et al.  Poly(3,4-ethylenedioxythiophene) nanorods grown on graphene oxide sheets as electrochemical sensing platform for rutin , 2015 .

[22]  D. M. Fernandes,et al.  Novel electrochemical sensor based on N-doped carbon nanotubes and Fe3O4 nanoparticles: simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid. , 2014, Journal of colloid and interface science.

[23]  Wojciech Domagala,et al.  Electrochemical overoxidation of poly(3,4-ethylenedioxythiophene)—PEDOT studied by means of in situ ESR spectroelectrochemistry , 2005 .

[24]  M. Noroozifar,et al.  Sensitive and selective determination of uric acid in real samples by modified glassy carbon electrode with holmium fluoride nanoparticles/multi-walled carbon nanotube as a new biosensor , 2013 .

[25]  Zahra Abedi,et al.  A highly sensitive electrochemical sensor for simultaneous detection of uric acid, xanthine and hypoxanthine based on poly(l-methionine) modified glassy carbon electrode , 2013 .

[26]  Masanori Okumura,et al.  Raman spectral changes of PEDOT-PSS in polymer light-emitting diodes upon operation , 2005 .

[27]  Pierre Temple-Boyer,et al.  Voltammetric microsensor using PEDOT-modified gold electrode for the simultaneous assay of ascorbic and uric acids , 2010 .

[28]  Yücel Şahin,et al.  Preparation of selective and sensitive electrochemically treated pencil graphite electrodes for the determination of uric acid in urine and blood serum. , 2010, Biosensors & bioelectronics.

[29]  F. R. A. Aquino Neto,et al.  Uric acid changes in urine and plasma: An effective tool in screening for purine inborn errors of metabolism and other pathological conditions , 2007, Journal of Inherited Metabolic Disease.

[30]  G. Láng,et al.  In situ monitoring of the electrochemical degradation of polymer films on metals using the bending beam method and impedance spectroscopy , 2012 .

[31]  Jixiao Wang,et al.  Electrochemical synthesis of polypyrrole nanowires on composite electrode , 2002 .

[32]  Hui Peng,et al.  Free radical scavenging properties of polypyrrole and poly(3,4-ethylenedioxythiophene) , 2008 .

[33]  B. Liu,et al.  Synthesis of positively charged CdTe quantum dots and detection for uric acid. , 2011, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[34]  Serban Peteu,et al.  Nanostructured poly(3,4-ethylenedioxythiophene)-metalloporphyrin films: improved catalytic detection of peroxynitrite. , 2010, Biosensors & bioelectronics.

[35]  V. Yegnaraman,et al.  Chemical Synthesis of PEDOT–Au Nanocomposite , 2007, Nanoscale Research Letters.

[36]  V. Yegnaraman,et al.  Determination of Uric Acid in the Presence of Ascorbic Acid Using Poly(3,4‐ethylenedioxythiophene)‐Modified Electrodes , 2005 .

[37]  J. Kan,et al.  Selective uricase biosensor based on polyaniline synthesized in ionic liquid , 2007 .

[38]  P. Gros,et al.  Evidence of an EC’ mechanism occurring during the simultaneous assay of ascorbic and uric acids on poly(3,4-ethylenedioxythiophene) modified gold microsensor , 2011 .

[39]  Jean-Louis Marty,et al.  Screen-printed poly(3,4-ethylenedioxythiophene) (PEDOT): A new electrochemical mediator for acetylcholinesterase-based biosensors. , 2010, Talanta.