The anatomical organization of hindlimb motoneurons in the lumbar spinal cord of the frog, Rana catesbiana

The precise location of lumbar spinal motoneurons was studied in the bullfrog by observing the retrograde reaction following injury to nerves which innervate different hindlimb muscles. The results were confirmed and their usefulness for physiological studies was demonstrated by iontophoretically injecting dye through an intracellular recording micropipette into motoneurons which were identified by antidromic spike invasion. The effects of plexus variation upon the location of motoneuron pools was considered in order to standardize the results and make possible comparisons between different frogs.

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