Identification and sequence analysis of the hupR1 gene, which encodes a response regulator of the NtrC family required for hydrogenase expression in Rhodobacter capsulatus

The hupR1 gene from Rhodobacter capsulatus was cloned and sequenced. It can encode a protein of 53,843 Da which shares significant similarity with several transcriptional regulators and activates transcription of the structural hupSL genes of [NiFe]hydrogenase, as shown by the use of a translational fusion of lacZ with the hupSL promoter. A Hup- mutant having a point mutation in the hupR1 gene is described.

[1]  J. Willison,et al.  Expression of regulatory nif genes in Rhodobacter capsulatus , 1991, Journal of bacteriology.

[2]  J. Wall,et al.  Clustering of genes necessary for hydrogen oxidation in Rhodobacter capsulatus , 1991, Journal of bacteriology.

[3]  A. Böck,et al.  Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli , 1990, Molecular microbiology.

[4]  A. Contreras,et al.  The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae , 1990, Molecular microbiology.

[5]  A. Ninfa,et al.  Protein phosphorylation and regulation of adaptive responses in bacteria. , 1989, Microbiological reviews.

[6]  R. Rappuoli,et al.  Families of bacterial signal‐transducing proteins , 1989, Molecular microbiology.

[7]  A. H. Stouthamer,et al.  Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12 , 1989, Journal of bacteriology.

[8]  F. Ausubel,et al.  The central domain of Rhizobium meliloti NifA is sufficient to activate transcription from the R. meliloti nifH promoter , 1989, Journal of bacteriology.

[9]  M. Simon,et al.  Protein phosphorylation in chemotaxis and two-component regulatory systems of bacteria. , 1989, The Journal of biological chemistry.

[10]  A. Chakrabarty,et al.  The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes , 1989, Journal of bacteriology.

[11]  J. Risler,et al.  Amino acid substitutions in structurally related proteins. A pattern recognition approach. Determination of a new and efficient scoring matrix. , 1988, Journal of molecular biology.

[12]  F. Corpet.  Multiple sequence alignment with hierarchical clustering. , 1988, Nucleic acids research.

[13]  D. Kahn,et al.  Cascade regulation of nif gene expression in Rhizobium meliloti , 1988, Cell.

[14]  J. Wootton,et al.  Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. , 1986, The EMBO journal.

[15]  P. Matsumura,et al.  Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein , 1984, Journal of bacteriology.

[16]  P. Vignais,et al.  The membrane-bound hydrogenase of Rhodopseudomonas capsulata is inducible and contains nickel , 1983 .

[17]  J. Walker,et al.  Distantly related sequences in the alpha‐ and beta‐subunits of ATP synthase, myosin, kinases and other ATP‐requiring enzymes and a common nucleotide binding fold. , 1982, The EMBO journal.

[18]  B. C. Kelley,et al.  Hydrogenase activity in Rhodopseudomonas capsulata: relationship with nitrogenase activity , 1980, Journal of bacteriology.

[19]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[20]  B. Marrs.  Genetic recombination in Rhodopseudomonas capsulata. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Willison,et al.  Expression ofRegulatory nifGenesinRhodobacter capsulatus , 1991 .

[22]  L. M. Albright,et al.  Prokaryotic signal transduction mediated by sensor and regulator protein pairs. , 1989, Annual review of genetics.

[23]  Florence Corpet,et al.  Multiple sequence alignment withhierarchical clustering , 1988 .

[24]  P. Vignais,et al.  Cloning of DNA fragments carrying hydrogenase genes of Rhodopseudomonas capsulata. , 1986, Biochimie.

[25]  C. Yanisch-Perron,et al.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. , 1985, Gene.

[26]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[27]  Christus,et al.  A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins , 2022 .