The research on biotransformation of praziquantel.

Praziquantel (PZQ), a broad spectrum antihelmintic drug, is extensively metabolized in the liver, yielding mainly monohydroxylated and dihydroxylated phase-I-metabolites. However, the exact chemical structures of the most metabolites are still unknown. In the presented research, three types of PZQ biotransformations were performed: biotransformation with the whole cells of Saccharomyces cerevisiae, with cytochrome c from Saccharomyces cerevisiae and with microsomes isolated from Saccharomyces cerevisiae. To describe the obtained metabolites TLC, RP-TLC, and HPLC were used.