BTK as a Mediator of Radiation-Induced Apoptosis in DT-40 Lymphoma B Cells

Bruton's tyrosine kinase (BTK) is a member of the SRC-related TEC family of protein tyrosine kinases (PTKs). DT-40 lymphoma B cells, rendered BTK-deficient through targeted disruption of the btk gene by homologous recombination knockout, did not undergo radiation-induced apoptosis, but cells with disrupted lyn or syk genes did. Introduction of the wild-type, or a SRC homology 2 domain or a plecstrin homology domain mutant (but not a kinase domain mutant), human btk gene into BTK-deficient cells restored the apoptotic response to radiation. Thus, BTK is the PTK responsible for triggering radiation-induced apoptosis of lymphoma B cells, and its kinase domain is indispensable for the apoptotic response.

[1]  D. Vaux,et al.  The molecular biology of apoptosis. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[2]  A. Burkhardt,et al.  Physical and Functional Interactions between Lyn and p34 Kinases in Irradiated Human B-cell Precursors (*) , 1996, The Journal of Biological Chemistry.

[3]  J. Kinet,et al.  Activation of BTK by a Phosphorylation Mechanism Initiated by SRC Family Kinases , 1996, Science.

[4]  N. Tumer,et al.  In vitro and in vivo antileukemic activity of B43-pokeweed antiviral protein against radiation-resistant human B-cell precursor leukemia cells. , 1995, Blood.

[5]  K. Sada,et al.  Role of the Syk autophosphorylation site and SH2 domains in B cell antigen receptor signaling , 1995, The Journal of experimental medicine.

[6]  Tony Pawson,et al.  Syk tyrosine kinase required for mouse viability and B-cell development , 1995, Nature.

[7]  Victor L. J. Tybulewicz,et al.  Perinatal lethality and blocked B-cell development in mice lacking the tyrosine kinase Syk , 1995, Nature.

[8]  T. Yamamoto,et al.  Impaired proliferation of peripheral B cells and indication of autoimmune disease in lyn-deficient mice. , 1995, Immunity.

[9]  S. Stacker,et al.  Multiple defects in the immune system of Lyn-deficient mice, culminating in autoimmune disease , 1995, Cell.

[10]  J. Fargnoli,et al.  Src family protein tyrosine kinases induce autoactivation of Bruton's tyrosine kinase , 1995, Molecular and cellular biology.

[11]  T. Kurosaki,et al.  Requirement of phospholipase C-gamma 2 activation in surface immunoglobulin M-induced B cell apoptosis , 1995, The Journal of experimental medicine.

[12]  R. Perlmutter,et al.  Impaired expansion of mouse B cell progenitors lacking Btk. , 1995, Immunity.

[13]  F. Alt,et al.  Defective B cell development and function in Btk-deficient mice. , 1995, Immunity.

[14]  Y. Hirabayashi,et al.  Inhibition of tumor necrosis factor alpha- and ceramide-induced internucleosomal DNA fragmentation by herbimycin A in U937 cells. , 1995, Biochemical and biophysical research communications.

[15]  J. Jin,et al.  In vitro and in vivo activity of topotecan against human B-lineage acute lymphoblastic leukemia cells. , 1995, Blood.

[16]  R. Rowley,et al.  Syk protein-tyrosine kinase is regulated by tyrosine-phosphorylated Ig alpha/Ig beta immunoreceptor tyrosine activation motif binding and autophosphorylation , 1995, The Journal of Biological Chemistry.

[17]  T. Kurosaki,et al.  Tyrosine Phosphorylation of Shc Is Mediated through Lyn and Syk in B Cell Receptor Signaling (*) , 1995, The Journal of Biological Chemistry.

[18]  A. Burkhardt,et al.  Biotherapy of B-cell precursor leukemia by targeting genistein to CD19-associated tyrosine kinases , 1995, Science.

[19]  J. Bluestone,et al.  Identification of Rlk, a Novel Protein Tyrosine Kinase with Predominant Expression in the T Cell Lineage (*) , 1995, The Journal of Biological Chemistry.

[20]  T. Eisner,et al.  Chemical ecology. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[21]  A. DeFranco,et al.  Transmembrane signaling by antigen receptors of B and T lymphocytes. , 1995, Current opinion in cell biology.

[22]  J. Bolen.  Protein tyrosine kinases in the initiation of antigen receptor signaling. , 1995, Current opinion in immunology.

[23]  C. Larsson,et al.  BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2. , 1994, Oncogene.

[24]  H. Hanafusa,et al.  Functional analysis of Csk in signal transduction through the B-cell antigen receptor , 1994, Molecular and cellular biology.

[25]  K. Isselbacher,et al.  Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Nagataki,et al.  Prevention of anti-CD3 monoclonal antibody-induced thymic apoptosis by protein tyrosine kinase inhibitors. , 1994, Journal of immunology.

[27]  R. Hendriks,et al.  B-cell antigen receptor stimulation activates the human Bruton's tyrosine kinase, which is deficient in X-linked agammaglobulinemia. , 1994, The Journal of biological chemistry.

[28]  J. Fargnoli,et al.  Temporal differences in the activation of three classes of non-transmembrane protein tyrosine kinases following B-cell antigen receptor surface engagement. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[29]  K. Bhalla,et al.  Evidence for involvement of tyrosine phosphorylation in taxol-induced apoptosis in a human ovarian tumor cell line. , 1994, Biochemical pharmacology.

[30]  T. Kawakami,et al.  The pleckstrin homology domain of Bruton tyrosine kinase interacts with protein kinase C. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[31]  R. Nelson,et al.  Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[32]  T J Gibson,et al.  PH domain: the first anniversary. , 1994, Trends in biochemical sciences.

[33]  C. M. Eischen,et al.  Tyrosine kinase activation provides an early and requisite signal for Fas-induced apoptosis. , 1994, Journal of immunology.

[34]  M. Vihinen,et al.  Deletion within the Src homology domain 3 of Bruton's tyrosine kinase resulting in X-linked agammaglobulinemia (XLA) , 1994, Journal of Experimental Medicine.

[35]  M. Yoshinari,et al.  Selective inhibition of dexamethasone-induced apoptosis in rat thymocytes by herbimycin A. , 1994, Biochemical and biophysical research communications.

[36]  Y. Ohta,et al.  TXK, a novel human tyrosine kinase expressed in T cells shares sequence identity with Tec family kinases and maps to 4p12. , 1994, Human molecular genetics.

[37]  M. Conley.  X-linked immunodeficiencies. , 1994, Current opinion in genetics & development.

[38]  O. Witte,et al.  Brief report: a point mutation in the SH2 domain of Bruton's tyrosine kinase in atypical X-linked agammaglobulinemia. , 1994, The New England journal of medicine.

[39]  R. Ashman,et al.  Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B cell apoptosis. , 1994, Journal of immunology.

[40]  T. Yamamoto,et al.  Syk activation by the Src-family tyrosine kinase in the B cell receptor signaling , 1994, The Journal of experimental medicine.

[41]  A. Hata,et al.  Tyrosine kinases Lyn and Syk regulate B cell receptor‐coupled Ca2+ mobilization through distinct pathways. , 1994, The EMBO journal.

[42]  Dan R. Littman,et al.  Signal transduction by lymphocyte antigen receptors , 1994, Cell.

[43]  D. Bredesen,et al.  Bcl-2 inhibition of neural death: decreased generation of reactive oxygen species. , 1993, Science.

[44]  Z. Oltvai,et al.  Bcl-2 functions in an antioxidant pathway to prevent apoptosis , 1993, Cell.

[45]  A. Burkhardt,et al.  Signal transduction through the CD19 receptor during discrete developmental stages of human B-cell ontogeny. , 1993, The Journal of biological chemistry.

[46]  D. Housman,et al.  p53-dependent apoptosis modulates the cytotoxicity of anticancer agents , 1993, Cell.

[47]  G. Mills,et al.  Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q. , 1993, Blood.

[48]  J. Cambier,et al.  Mapping of sites on the Src family protein tyrosine kinases p55blk, p59fyn, and p56lyn which interact with the effector molecules phospholipase C-gamma 2, microtubule-associated protein kinase, GTPase-activating protein, and phosphatidylinositol 3-kinase , 1993, Molecular and cellular biology.

[49]  C. Thompson,et al.  bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death , 1993, Cell.

[50]  D J Rawlings,et al.  Mutation of unique region of Bruton's tyrosine kinase in immunodeficient XID mice. , 1993, Science.

[51]  T. Yagi,et al.  Constitutive activation of Src family kinases in mouse embryos that lack Csk , 1993, Cell.

[52]  G. Schieven,et al.  Tyrosine phosphorylation is a mandatory proximal step in radiation-induced activation of the protein kinase C signaling pathway in human B-lymphocyte precursors , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[53]  T. Kawakami,et al.  Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells. , 1993, Biochemical and biophysical research communications.

[54]  C. Song,et al.  Radiation damage repair capacity of primary clonogenic blasts in acute lymphoblastic leukemia. , 1993, Cancer research.

[55]  C. Song,et al.  Intrinsic radiation resistance of primary clonogenic blasts from children with newly diagnosed B-cell precursor acute lymphoblastic leukemia. , 1993, The Journal of clinical investigation.

[56]  N. Copeland,et al.  Expression of a novel form of Tec kinase in hematopoietic cells and mapping of the gene to chromosome 5 near Kit. , 1993, Oncogene.

[57]  Ornella Parolini,et al.  Deficient expression of a B cell cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia , 1993, Cell.

[58]  L. Berg,et al.  Developmental regulation of a murine T-cell-specific tyrosine kinase gene, Tsk. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[59]  D. Bentley,et al.  The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases , 1993, Nature.

[60]  J. Bolen,et al.  The Src family of tyrosine protein kinases in hemopoietic signal transduction , 1992, Stem cells.

[61]  S. Desiderio,et al.  itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[62]  T. Pawson,et al.  SH2 and SH3 domains: From structure to function , 1992, Cell.

[63]  G. Schieven,et al.  Ionizing radiation stimulates unidentified tyrosine-specific protein kinases in human B-lymphocyte precursors, triggering apoptosis and clonogenic cell death. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[64]  B. Sefton,et al.  Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases , 1992, Molecular and cellular biology.

[65]  J. Hutchcroft,et al.  Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor. , 1992, The Journal of biological chemistry.

[66]  V. Fadok,et al.  Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. , 1992, Journal of immunology.

[67]  J. Brugge,et al.  Phosphorylation of c-Src on tyrosine 527 by another protein tyrosine kinase. , 1991, Science.

[68]  A. Burkhardt,et al.  Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[69]  T Pawson,et al.  SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins. , 1991, Science.

[70]  H. Mano,et al.  A novel protein-tyrosine kinase, tec, is preferentially expressed in liver. , 1990, Oncogene.

[71]  R. Schlegel,et al.  Plasma membrane lipid organization and the adherence of differentiating lymphocytes to macrophages , 1989, Journal of cellular physiology.

[72]  J. Cohen,et al.  Gene induction by gamma-irradiation leads to DNA fragmentation in lymphocytes. , 1987, Journal of immunology.

[73]  A. Wyllie,et al.  Cell death: the significance of apoptosis. , 1980, International review of cytology.