Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

[1]  R. Fernández-Lafuente,et al.  Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads. , 2021, Journal of biotechnology.

[2]  R. Fernández-Lafuente,et al.  Immobilization and stabilization of d-hydantoinase from Vigna angularis and its use in the production of N-carbamoyl-d-phenylglycine. Improvement of the reaction yield by allowing chemical racemization of the substrate , 2020 .

[3]  R. Fernández-Lafuente,et al.  Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions , 2020 .

[4]  A. Illanes,et al.  Synthesis of Lactulose in Continuous Stirred Tank Reactor With β-Galactosidase of Apergillus oryzae Immobilized in Monofunctional Glyoxyl Agarose Support , 2020, Frontiers in Bioengineering and Biotechnology.

[5]  R. Fernández-Lafuente,et al.  Glyoxyl-Activated Agarose as Support for Covalently Link Novo-Pro D: Biocatalysts Performance in the Hydrolysis of Casein , 2020 .

[6]  R. Fernández-Lafuente,et al.  Parameters necessary to define an immobilized enzyme preparation , 2020, Process Biochemistry.

[7]  J. Dutton,et al.  Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution—An Optimization and Comparative Study , 2020, Frontiers in Bioengineering and Biotechnology.

[8]  R. Vanna,et al.  Immobilization of γ‐Glutamyl Transpeptidase from Equine Kidney for the Synthesis of kokumi Compounds , 2020, ChemCatChem.

[9]  R. Fernández-Lafuente,et al.  Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts. , 2019, International journal of biological macromolecules.

[10]  A. Illanes,et al.  Continuous enzymatic synthesis of lactulose in packed-bed reactor with immobilized Aspergillus oryzae β-galactosidase. , 2019, Bioresource technology.

[11]  A. Illanes,et al.  Effect of particle size and enzyme load on the simultaneous reactions of lactose hydrolysis and transgalactosylation with glyoxyl-agarose immobilized β-galactosidase from Aspergillus oryzae , 2018, Process Biochemistry.

[12]  J. Rocha-Martín,et al.  Stabilization of Enzymes by Multipoint Covalent Attachment on Aldehyde-Supports: 2-Picoline Borane as an Alternative Reducing Agent , 2018, Catalysts.

[13]  J. Rocha-Martín,et al.  Sequential hydrolysis of commercial casein hydrolysate by immobilized trypsin and thermolysin to produce bioactive phosphopeptides , 2018 .

[14]  C. Bernal,et al.  Selective and eco-friendly synthesis of lipoaminoacid-based surfactants for food, using immobilized lipase and protease biocatalysts. , 2018, Food chemistry.

[15]  R. Fernández-Lafuente,et al.  Effect of high salt concentrations on the stability of immobilized lipases: Dramatic deleterious effects of phosphate anions , 2017 .

[16]  O. Barbosa,et al.  Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins , 2017 .

[17]  R. Fernández-Lafuente,et al.  Agarose and Its Derivatives as Supports for Enzyme Immobilization , 2016, Molecules.

[18]  D. Freire,et al.  Strategies of covalent immobilization of a recombinant Candida antarctica lipase B on pore-expanded SBA-15 and its application in the kinetic resolution of (R,S)-Phenylethyl acetate , 2016 .

[19]  C. Ortiz,et al.  Inactivation of immobilized trypsin under dissimilar conditions produces trypsin molecules with different structures , 2016 .

[20]  F. López‐Gallego,et al.  Immobilization of Proteins on Highly Activated Glyoxyl Supports: Dramatic Increase of the Enzyme Stability via Multipoint Immobilization on Pre-existing Carriers , 2015 .

[21]  R. Monti,et al.  Covalent immobilization of laccase in green coconut fiber and use in clarification of apple juice , 2015 .

[22]  O. Barbosa,et al.  Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin , 2015 .

[23]  A. Illanes,et al.  Immobilization of Bacillus circulans β-galactosidase and its application in the synthesis of galacto-oligosaccharides under repeated-batch operation , 2013 .

[24]  C. Bernal,et al.  Hierarchical meso-macroporous silica grafted with glyoxyl groups: opportunities for covalent immobilization of enzymes. , 2013, New biotechnology.

[25]  F. Moyano,et al.  Enzymatic hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide by α-chymotrypsin in DMSO-water/AOT/n-heptane reverse micelles. A unique interfacial effect on the enzymatic activity. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[26]  V. Grazú,et al.  Tailor-made design of penicillin G acylase surface enables its site-directed immobilization and stabilization onto commercial mono-functional epoxy supports , 2012 .

[27]  J. Rocha-Martín,et al.  Stabilization of a highly active but unstable alcohol dehydrogenase from yeast using immobilization and post-immobilization techniques , 2012 .

[28]  H. F. de Castro,et al.  Immobilization of a Commercial Lipase from Penicillium camembertii (Lipase G) by Different Strategies , 2011, Enzyme research.

[29]  J. Pedroche,et al.  Improvement of functional properties of chickpea proteins by hydrolysis with immobilised Alcalase , 2010 .

[30]  J. García,et al.  Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli , 2010 .

[31]  Andrea Komesu,et al.  Immobilization and Stabilization of Xylanase by Multipoint Covalent Attachment on Agarose and on Chitosan Supports , 2010, Applied biochemistry and biotechnology.

[32]  R. Fernández-Lafuente.  Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation , 2009 .

[33]  R. Rodrigues,et al.  The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment , 2009 .

[34]  C. Kutzbach,et al.  Preparation and General Properties of Crystalline Penicillin Acylase from Escherichia coli ATCC 11 105 , 2009, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[35]  Roberto Fernandez-Lafuente,et al.  Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination , 2009 .

[36]  F. Cava,et al.  Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization , 2009 .

[37]  R. Fernández-Lafuente,et al.  Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose. , 2008, Biomacromolecules.

[38]  R. Giordano,et al.  Multipoint covalent immobilization of microbial lipase on chitosan and agarose activated by different methods , 2008 .

[39]  R. Torres,et al.  Advances in the design of new epoxy supports for enzyme immobilization-stabilization. , 2007, Biochemical Society transactions.

[40]  R. Fernández-Lafuente,et al.  Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability , 2007 .

[41]  R. Fernández-Lafuente,et al.  Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii , 2007 .

[42]  R. Fernández-Lafuente,et al.  Stabilization of different alcohol oxidases via immobilization and post immobilization techniques , 2007 .

[43]  G. Zanin,et al.  Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose , 2006 .

[44]  R. Fernández-Lafuente,et al.  Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose. , 2006, Journal of biotechnology.

[45]  R. Fernández-Lafuente,et al.  Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins , 2006 .

[46]  M. Alaiz,et al.  Immobilization of angiotensin-converting enzyme on glyoxyl-agarose. , 2006, Journal of agricultural and food chemistry.

[47]  R. Fernández-Lafuente,et al.  Glyoxyl agarose as a new chromatographic matrix , 2006 .

[48]  R. Fernández-Lafuente,et al.  Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports. , 2006, Biomacromolecules.

[49]  R. Fernández-Lafuente,et al.  Preparation of a very stable immobilized biocatalyst of glucose oxidase from Aspergillus niger. , 2006, Journal of biotechnology.

[50]  O. Abián,et al.  Some special features of glyoxyl supports to immobilize proteins , 2005 .

[51]  S. Ichikawa,et al.  Immobilization and stabilization of pullulanase from Klebsiella pneumoniae by a multipoint attachment method using activated agar gel supports , 2005 .

[52]  R. Fernández-Lafuente,et al.  Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports. , 2005, Journal of biotechnology.

[53]  R. Giordano,et al.  Kinetic model of the hydrolysis of polypeptides catalyzed by Alcalase® immobilized on 10% glyoxyl-agarose , 2005 .

[54]  A. Albertini,et al.  Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis. , 2004, Biomacromolecules.

[55]  J. García,et al.  Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment , 2004, Applied and Environmental Microbiology.

[56]  H. Toogood,et al.  Immobilisation of the Thermostable l -aminoacylase from Thermococcus litoralis to Generate a Reusable Industrial Biocatalyst , 2002 .

[57]  J. Mansfeld,et al.  Site‐specific and random immobilization of thermolysin‐like proteases reflected in the thermal inactivation kinetics , 2000, Biotechnology and applied biochemistry.

[58]  O. Abián,et al.  Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. , 2000, Biomacromolecules.

[59]  C. Mateo,et al.  Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment* , 2000, Enzyme and microbial technology.

[60]  A. Sali,et al.  Mouse Mast Cell Protease 9, a Novel Member of the Chromosome 14 Family of Serine Proteases that is Selectively Expressed in Uterine Mast Cells* , 1997, The Journal of Biological Chemistry.

[61]  R. Fernández-Lafuente,et al.  Hyperstabilization of a thermophilic esterase by multipoint covalent attachment , 1995 .

[62]  M. Alaiz,et al.  Amino acid analysis by high-performance liquid chromatography after derivatization with diethyl ethoxymethylenemalonate. , 1992, Journal of chromatography.

[63]  C. Rosell,et al.  Immobilization‐stabilization of α‐chymotrypsin by covalent attachment to aldehyde‐agarose gels , 1991 .

[64]  R. Fernández-Lafuente,et al.  Immobilization-stabilization of Penicillin G acylase fromEscherichia coli , 1990, Applied biochemistry and biotechnology.

[65]  R. M. Blanco,et al.  Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives , 1989 .

[66]  J. Calvete,et al.  Immobilization-stabilization of enzymes; variables that control the intensity of the trypsin (amine)-agarose (aldehyde) multipoint attachment , 1989 .

[67]  J. Guisán.  Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes , 1988 .

[68]  R. M. Blanco,et al.  Protecting effect of competitive inhibitors during very intense insolubilized enzyme-activated support multipoint attachments: trypsin (amine)-agarose (aldehyde) system , 1988 .

[69]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[70]  R. Rodrigues,et al.  HETEROFUNCTIONAL SUPPORTS IN ENZYME IMMOBILIZATION: 1 FROM TRADITIONAL IMMOBILIZATION PROTOCOLS TO OPPORTUNITIES IN 2 TUNING ENZYME PROPERTIES , 2014 .

[71]  A. Illanes,et al.  Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae , 2011 .

[72]  G. Zanin,et al.  Immobilization–stabilization of glucoamylase: Chemical modification of the enzyme surface followed by covalent attachment on highly activated glyoxyl-agarose supports , 2011 .

[73]  R. Giordano,et al.  Immobilization and stabilization of microbial lipases by multipoint covalent attachment on aldehyde-resin affinity: Application of the biocatalysts in biodiesel synthesis , 2011 .

[74]  R. Fernández-Lafuente,et al.  Preparation of a Stable Biocatalyst of Bovine Liver Catalase Using Immobilization and Postimmobilization Techniques , 2003, Biotechnology progress.

[75]  R. Fernández-Lafuente,et al.  Hydrolysis of Proteins by Immobilized‐Stabilized Alcalase‐Glyoxyl Agarose , 2003, Biotechnology progress.

[76]  R. Fernández-Lafuente,et al.  Design of New Immobilized‐Stabilized Carboxypeptidase A Derivative for Production of Aromatic Free Hydrolysates of Proteins , 2003, Biotechnology progress.

[77]  R. Fernández-Lafuente,et al.  Modification of enzyme properties by the use of inhibitors during their stabilisation by multipoint covalent attachment , 1995 .

[78]  G. Gomori.  Preparation of Buffers for Use in Enzyme Studies , 1955 .